J. Planar Chromatogr. 28, 218-222 (2015). HPTLC of Tripterygium wilfordii from QiYi capsule on silica gel with chloroform - diethyl ether 6.2:1. Detection at UV 365 nm. 9 common peaks were selected to evaluate the similarities of 10 batches. The HPTLC method revealed a standardized consistency and reliable quality of QiYi.

by the combination of high-performance thin-layer chromatography with direct bioautography and mass spectrometry. J. of Chromatogr. A 1422, 310-317 (2015). Investigation of two tansy (Tanacetum vulgare L.) essential oils, obtained by steam distillation of the capitula with subsequent liquid-liquid extraction (oil 1) or with use of an auxiliary phase for the trapping of the steam components (oil 2), against Bacillus subtilis F1276, B. subtilis spizizenii (DSM 618), Xanthomonas euvesicatoria, Pseudomonas syringae pv. maculicola, Ralstonia solanacearum strain GMI1000 and Aliivibrio fischeri by using the coupling of HPTLC to direct bioautography (HPTLC-DB). Oil 2 was richer in components and provided more inhibition zones due to the advanced extraction process. Identification of the main bioactive components by scanning HPTLC-direct analysis in real time mass spectrometry (HPTLC-DART-MS) and solid-phase microextraction GC electron impact MS (SPME-GC-EI-MS) as cis- and trans-chrysanthenol as well as trans-chrysanthenyl acetate. The results indicated that cis-chrysanthenol exhibited antibacterial effects against all tested bacteria. Trans-chrysanthenol inhibited B. subtilis, R. solanacearum and A. fischeri, and trans-chrysanthenyl acetate was an inhibitor for X. euvesicatoria, R. solanacearum and A. fischeri. However, the ionization characteristics and the recorded mass spectra showed that DART is a softer ionization technique than EI and more affected by ambient conditions and thus prone to additional oxidation products, although HPTLC-DART-MS resulted in a comparable fragmentation.

J. of China Pharm. 23 (8), 26-28 (2014). Compound Pipajiegeng ointment is a herbal TCM preparation for chronic cough, upper respiratory tract inflammation, and bronchitis. For quality control, TLC (1) of Eriobotrya japonica Thunb., on silica gel with chloroform – ethyl acetate – methanol – water 16:38:22:9, detection by spraying with 5 % phosphomolybdic acid in ethanol and heating at 105 °C until the zones are visible; (2) of Pericarpium Papaveris, on 2 % NaOH-containing silica gel with toluene – acetone – ethanol - ammonium hydroxide 20:19:3:1, detection by spraying with 5 % potassium iodobismuthate solution and viewing in daylight; (3) of Platycodon grandiflorus (Jacq.) A. DC., on 0.3 % NaOH-containing silica gel with cyclohexane – chloroform – methanol 15:11:1, detection by spraying with 25 % phosphomolybdic acid in ethanol and heating at 105 °C until the zones are visible; (4) of menthol, on silica gel with n-hexane – ethyl acetate 9:2, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 4:1 and heating until the zones are visible. Quantification of morphine by HPLC.

J. of Modern Trad. Chinese Med. 16 (2), 144-150 (2014). Shushenling Jiaonang capsule is a herbal TCM for the treatment of neurasthenia, neurosis, menopausal syndrome, etc. For quality control, TLC (1) for Salvia miltiorrhiza, on silica gel with toluene – ethyl acetate 19:1, detection under white light; (2) Schisandra chinensis, on silica gel with petroleum ether (30-60 ºC) – ethyl formate – formic acid 15:5:1, detection under UV 254 nm; (3) for Glycyrrhiza uralensis, on silica gel with toluene – ethyl acetate – methanol 7:3:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones are clearly visualized, evaluation under white light; (4) Panax ginseng, on silica gel with chloroform – methanol – water 13:7:2, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC until the zones are clearly visualized, evaluation under white light.

Planta Med. 83(03/04), 334-340 (2017). Preparative layer chromatography on silica gel was used to isolate, from flash column subfractions of ethyl acetate extracts of Lepisanthes senegalensis stem or roots: a) with chloroform – ethyl acetate 3:37 an acetyl-caffeoylbutelin from stem; b) with hexane – ethyl acetate 4:1 two coumaroyl-22-hydroxy-hopanes from root; c) with dichloromethane – ethyl acetate 19:1 a caffeoyl-lupeol from root.

J. Chromatogr. A 1524, 266-272 (2017). Demonstration of the antibacterial profiling of Onopordum acanthium L. leaf extract and subsequent targeted identification of active compounds. Investigation of the extract against eight bacterial strains including two plant and three human pathogens and a soil, a marine and a probiotic human gut bacteria by TLC and off-line overpressured layer chromatography (OPLC) coupled with direct bioautography. Transfer of antibacterial fractions obtaining infusion-transfusion OPLC to HPLC-MS/MS analysis resulted in the characterization of three active compounds, two of them were identified as linoleic and linolenic acid. Adoption of OPLC method to preparative-scale flash chromatography for the isolation of the third active compound, which was identified after a further semi-preparative HPLC purification as the germacranolide, sesquiterpene lactone onopordopicrin. Pure onopordopicrin exhibited antibacterial activity that was specified as minimal inhibitory concentration in the liquid phase as well.

J. Chromatogr. 312, 357-385 (1984). Two-dimensional TLC of dansylated basic nitrogen fractions (BNF) on silica with cyclohexane - ethyl acetate 2:3 and with benzene - triethylamine 5:1. Detection by UV 366 nm. Dansylation of the plant amines was carried out prior to TLC with DNS chloride in acetone (5 mg/ml) in the presence of excess solid NaHCO3. Standards for comparison were separated with chloroform - butylacetate 8:3 and with cyclohexane - benzene - methanol 2:7:1. Rf values of more than 80 plant amines are reported.

Pharm. Anal. (Yaowu Fenxi Zazhi) 5, 18-20 (1985). (Chinese) (TLC-UV spectrophotometric determination of paeoniflorin in Chinese paeonia medicinal herbs and Ming Mu Xiao Yao San preparations.) TLC of paeoniflorin on silica. The spot was eluted with ethanol. Determination by spectrophotometry at 234 nm. Approx. 100 % recovery.