Journal of Pharmacognosy and Phytochemistry 4 (2), 1-4 (2015). HPTLC of petroleum ether and ethanol extracts of aerial parts of Blumea lacera D.C. (Asteraceae), collected in the Gorakhpur district, with chloroform - benzene - formic acid 15:5:3. Evaluation in daylight revealed 11 zones, at least 9 of them at hRf between 3 and 47. The ethanol extract was purified by washing with water, chloroform and ethyl acetate successively, and then by fractionation on a silica gel (60-120 mesh) column; the benzene subfraction yielded, after exposure to iodine vapor, one yellow zone (hRf 41), identified (by IR, NMR and MS) as 3,5-dihydroxy-3’-methyl-6,7,4’-trimethoxyflavanone.

J. Ethnopharmacol. 181, 236-251 (2016). Analysis of the botanical description, geographical location, traditional and medicinal uses, phytochemistry and pharmacological investigation, toxicological aspects, patent information and clinical studies of Symplocos racemosa. The review described methods for the determination of loturine in the bark extracts of Symplocos racemosa on silica gel with chloroform – acetonitrile – triethylamine 7:5:2, quantitative determination by absorbance measurement at 280 nm.

CBS 114, 10-12 (2014). HPTLC of plant extracts on silica gel with either (1) dichloromethane – methanol – water 70:30:4 for polar extracts, (2) ethyl acetate – methanol – water – formic acid 50:10:7:1 for methanol extracts containing very polar compounds, or (3) toluene – ethyl acetate – formic acid 40:10:1 for lipophilic extracts. Detection by bioautography: Spraying with 2.5 mL substrate solution (0.047 g of L-DOPA mixed with 20 mL of phosphate buffer containing 1 % Triton X-100) and 3 mL of enzyme solution (mushroom tyrosinase in phosphate buffer, activity 400 U/mL), followed by incubation for 10 min at room temperature. Detection under UV 254 nm and 366 nm and white light. Positive results appeared as white inhibition zones on the dark plate background.

Phytochem. Anal. 27, 239-248 (2016). HPTLC of Echium vulgare from different sites (characterized by varied heavy metal contamination in the substrates) on silica gel with ethyl acetate – 98-100 % formic acid – glacial acetic acid – water 100:11:11:26. Qualitative identification under UV light at 366 nm. Pre-processing of TLC plate images was performed using open architecture software.

Planta Medica 82(18), 1546-1552 (2016). The fractionation of the butanol-soluble part of the methanol extract of Lysimachia ciliata was monitored through TLC on silica gel with chloroform – methanol – water 23:12:2, detection by spraying with sulfuric acid. The saponin-containing fractions were mixed and submitted to preparative TLC on silica gel (same mobile phase). Saponins, eluted with methanol from the water-sprayed layer, were analyzed by UPLC-ESI-MS/MS; two were identified as desglucoanagalloside B and anagallosaponin IV (29.1 % and 8.2 % of the saponin fraction, respectively).

J. Chromatogr. A 1490, 201-211 (2017). HPTLC for comparison of the phenolic profiles of polar extracts from Populus nigra L. (1), Populus alba L. (2) and Salix alba L. (3) buds. Five chemotypical patterns were distinguished after derivatization with Natural Products reagent and confirmed by principal component analysis. The HPTLC analysis was directly hyphenated to various microbiological and biochemical assays as well as spectrometric techniques, which directly linked to active molecules in the chromatograms. The results showed that polyvalent compounds were evident when all derivatization and activity assays were combined together. Detection of at least three antimicrobial compound zones using Aliivibrio fischeri and Bacillus subtilis bioassays and of one phyto-estrogen with the planar yeast estrogen screen in Populus buds. Detection of several inhibitors of acetyl- and butyrylcholinesterase and rabbit liver esterase in all samples. Bioactive compounds were assigned by HPTLC-MS, e.g. chrysin as selective cholinesterase inhibitor, and caffeic acid and galangin as antimicrobials in (1) and (2). The method is suitable to determine the botanical origin and quality of Populus bud extracts and propolis samples.

Synthesis of oxidative degradation products from the methylated component. Phenols of anacardium occidentale and other phenolic lipids: confirmation of the structure of the parent phenols and of a related material. Lipids 17, 561-569 (1982). Isolation of the degradation products of anacardic acid, cardol, 2-methyl-cardol and cardanol by TLC on silica with a) chloroform, b) chloroform ethyl acetate 95:5, c) chloroform - petrol ether 6:4. Detection and visualization acc. to J. Caplin and J. Tyman, J. Chem. Res. 5, 34-35, 11, 0321- 0351 (1982).

Shanghai 1st. Med. Coll.(Shanghai Diyi Yixueyuan Xuebao) 11, 284-287 (1984). (Chinese). (Evaluation of total alkaloids in Chinese traditional drugs fen fang ji by TLC-spectrophotometric method.) TLC of tetradrine on silica with methanol - chloroform 1:5. Detection by UV. Quantification by spectrophotometry of methanol - eluate at 282 nm.