Planta Med. 75, 132-136 (2009). Analytical and preparative TLC of 3’-hydroxy-4’,5,6,7-tetramethoxyflavone, 3’,5-dihydroxy-4’,6,7-trimethoxy-flavone (eupatorin), and 3’,4’,5,6,7-pentamethoxyflavonene (sinensetin) and methanolic plant extracts of Orthosiphon stamineus on silica gel with chloroform - ethyl acetate 7:3, and chloroform - ethyl acetate - acetic acid 30:70:1. Detection by spraying with anisaldehyde-sulfuric acid followed by heating.

J. Planar Chromatogr. 25, 251-258 (2012). TLC of trans-resveratrol (1), pterostilbene (2), and p-coumaric acid (3) in samples of recombinant bacterial cultures, food supplements, and wine on silica gel with n-hexane - ethyl acetate - formic acid 20:19:1. Quantitative determination by absorbance measurement at 286 nm (3) and 303 nm for (1) and (2).

J. of Yangtze Univ. (Nat. Sci., Med.) 9 (1), 64-65 (2012) . Angelica sinensis is a herbal TCM drug widely used in the preparations for gynaecological diseases, effective in promoting blood circulation, ease of pain and against inflammations. TLC of the extracts of the preparation on silica gel with n-hexane – ethyl acetate 9:1. Detection under UV 366 nm.

(Papaveraceae) tissues by TLC–in situ fluorography. J. Planar Chromatogr. 25, 358-360 (2012). TLC of berberine and sanguinarine on silica gel with n-butanol - water - ammonia 8:1:1. Detection by densitometry in fluorescence mode at 325 nm. The hRf of berberine and sanguinarine were 29 and 95, respectively.

J. of China Pharm. 21 (12), 15-16 (2012). The leaf of Microcos paniculata L. is a popular medicinal herbal drug used for cardiovascular protection, blood fat adjusting, and anti-aging. Presentation of methods for identifying the drug by TLC and FTIR. TLC of the extracts of the drug on silica gel 1) for kaempferide, with toluene – ethyl acetate – formic acid 9:3:1, detection by spraying with 3 % aluminiumchloride in ethanol followed by heating at 105 ºC for 5 min and viewing at UV 366 nm; 2) for vitexin and isovitexin, developed with ethyl acetate – methanol – water 100:17:13, detection by spraying with 3 % aluminiumchloride in ethanol followed by heating at 105 ºC for 5 min and viewing at UV 366 nm. The methods have been applied to 15 batches of real life sample available from different places of origin, and proved that TLC procedures as well as FTIR are simple, sensitive, reproducible and robust and suitable for the identification of the herbal drug.

Chinese J. of Guide for Trad. Chinese Med. & Pharm. 18 (5), 80-81 (2012). Weijianning capsules, a herbal TCM preparation for tonifying spleen and invigorating the stomach, is prescribed clinically to treat superficial, erosive and atrophic gastritis. Presentation of a TLC method for the quality control of the preparations optimized by investigating the sample extraction procedure and the mobile phases employed. TLC of the extracts on silica gel 1) for Radix Scrophulariae with ethyl acetate – ethanol – water 20:5:3, detection by spraying with a solution of 3 g vanillin – 100 mL ethanol – 0.5 mL sulfuric acid and heating at 105 °C until the zones were clearly visualized, evaluation in daylight; 2) for Fructus Amomi with n-hexane - ethyl acetate 3:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C until the zones were clearly visualized, evaluation in daylight. Identification by fingerprint comparison in parallel with the individual standard components.

CBS 110, 5-7 (2013). HPTLC of extracts of (1) Nauclea diderrichii, (2) Sarcocephalus pobeguinii, (3) Hua gabonii, (4) Morinda lucida, and (5) Momordica foetida on silica gel with A) toluene - ethyl acetate 19:1; B) chloroform - methnaol - water 35:15:2; C) ethyl acetate - acetic acid - formic acid - water 100:11:11:27, D) acetonitrilie - water - formic acid 15:4:1, and E) 1-butanol - acetic acid - water 7:1:2. For (1) mobile phases B and C were best suited, for (2) mobile phase B, for lipophilic compounds of the essential oil of (3) mobile phase A and for (4) and (5) mobile phase B. Bioautographic analysis using alpha- and beta-glucosidase enzym assays, acetylcholinesterase inhibition assay, and DPPH (2,2-diphenyl-1-picrylhydrazyl) radical reagent for detecting radical scavenging activity.

J. of Anhui Coll. of Trad. Chinese Med. 31 (4), 77-79 (2012). Wuzi Yanzong pills are a herbal TCM preparation for treating male impotence, sterility, and premature ejaculation. For quality control, TLC on silica gel 1) for Fructus Lycii and China Dodder, with cyclohexane – acetone 3:2, detection under UV 366 nm; 2) for Fructus Schisandrae Chinensis, with chloroform – butanone 30:1, detection under UV 254 nm. Quantification of schisantherin A by absorbance measurement at 230 nm. Linearity was between 0.25-1.10 µg/zone (r = 0.999, n=5). Plate-to-plate %RSD was 2.1 % (n=5) and 0.98 % within plate (n=5). Recovery (by standard addition) was 100.9 % (%RSD = 0.51, n=6).