60th Indian Pharmaceutical Congress PG-264 (2008). HPTLC of quercetin in acetone extracts of Psidium guajava leaves on silica gel with toluene - acetone - formic acid 38:10:5. Quantitative determination of quercetin by absorbance measurement at 364 nm. The correlation coefficient was 0.9847. There was a good correlation between peak area and corresponding concentration of quercetin. The proposed HPTLC method provided a good resolution of quercetin from other constituents present in acetone extract of tender leaves of Psidium guajava and can be used for the quantification of quercetin.

J. Pharm. Biomed. Anal. 47, 795-801 (2008). HPTLC of sesamin and sesamoline (the major lignans in sesamum oil and its herbal formulations) on silica gel with benzene - methanol 50:1 with chamber saturation. Quantitative determination by absorbance measurement at 290 nm. The identity of sesamin and sesamoline was confirmed by UV-VIS spectra, NMR and MS of the compounds obtained by scraping off from the plate and elution. For fingerprint analysis derivatization with 5 % methanolic sulphuric acid was performed, followed by heating at 100 °C for 20 min and densitometry at 450 nm.

International Seminar on Herbal Drug Research, PN-011 (2009). HPTLC of harmaline in alcoholic extracts of Peganum harmala on silica gel with chloroform - acetone - diethyl amine 5:4:1 in a saturated chamber at 25 °C. The hRf of harmaline was 50. Quantitative determination by absorbance measurement at 351 nm.

Abstract No. C-47, 61st IPC (2009). HPTLC of piperine and vasicine in polyherbal cough formulations on silica gel with dioxane - toluene - ethyl acetate - methanol - 25 % ammonia 15:20:10:10:3. Quantitative determination by absorbance measurement at 304 nm. The method was validated for accuracy, precision, LOD, LOQ, linearity and specificity and was found to be linear in the range of 2-10 µg/band for both vasicine and piperine.

Phytochem. Anal. 21, 174-179 (2010). HPTLC of tiliroside (1), methyl brevifolincarboxylate (2), and ellagic acid (3) in the aerial parts of Potentilla species on silica gel with toluene - ethyl formate - formic acid 6:4:1. Quantitative determination by absorbance measurement at 320 nm for (1), 287 nm for (2), and 280 nm for (3). The hRf values of (1), (2) and (3) were 9, 13, and 20, respectively. Linearity was between 50 and 500 ng/zone for (1), 50 and 520 ng/zone for (2), and 52 and 500 ng/zone for (3). The intra- and inter-day precisions (expressed in terms of CV %) were observed in the ranges 2.5-9.0 % and 2.4-11.1 %, respectively. LOD for (1) to (3) were 5, 10 and 34 ng/zone, respectively, while LOQ were 27, 17 and 44 ng/zone, respectively. The average recovery of (1), (2) and (3) was 101.1, 82.2 and 94.0 %, respectively.

Application to the detection of antimicrobial compounds from Cordia gilletii De Wild (Boraginaceae). J. Planar Chromatogr. 23, 245-249 (2010). TLC of methanol extracts of Cordia gilletii on silica gel with dichloromethane, dichloromethane - methanol 9:1 or 4:1, or petroleum ether - diethyl ether - acetic acid 90:10:1. Detection by spraying with 1 % vanillin in ethanol followed immediately by 10 % ethanolic sulfuric acid, and heating at 105 °C for 10 min. For bioautography, 1 mL of 0.5 McFarland microorganism suspension was diluted with 9 mL of the tested mixture of Mueller-Hilton broth and agar 9:1) at 37 °C. After distribution of the inoculated mixture over the plate and solidification of the medium at ambient temperature, the plate was incubated overnight at 37 °C. The bioautogram was subsequently sprayed with an aqueous solution of MTT 0.8 mg mL-1 and incubated at 37 °C for 4 h. Also application of the Bioluminex assay (a TLC detection method based on the natural bioluminescence of the non-pathogenic bacteria Vibrio fischerii). The bioluminescent bacterial coating was visualized by use of the Bioluminizer.

Acta Chromatographica 22 (2), 259-265 (2010), DOI:10.1556/AChrom.22.2010.2.8. Description of a new, simple, precise, and accurate method for quantification of (-)-epicatechin in the leaves of Cassia fistula by HPTLC on silica gel with toluene – ethyl acetate – formic acid – methanol 205:3:1:1. Quantification by densitometry at 280 nm. The linearity was in the range of 200–800 ng/band. Method precision was 1.4 %RSD and instrumental precision 1.1 %RSD. Recovery was 98.1 % and specificity regarding matrix was given.

J. Ethnopharmacol. 139, 142-148 (2012). HPTLC of 1,2-dihydroxy-3-pentadec-8-enylbenzene (A) and 1,2-dihydroxy-3-pentadeca-8,11-dienylbenzene (B) in the fruits of Semecarpus anacardium L. f. (Anacardiaceae) on RP-18 with acetonitrile - water 199:1. Detection by spraying with anisaldehyde - sulfuric acid reagent. The hRf values of (A) and (B) were 31 and 42, respectively. The method was combined with ESI-MS and NMR for compound identification.