IPA Convention, 2010, RA-PO 35. HPTLC of citicoline sodium in tablet formulation on silica gel with chloroform - methanol - water 3:7:3. The compound was well resolved with an hRf value of 53. Densitometric measurement at 280 nm. The method was linear in the range of 300-900 ng/band. HPLC analysis was performed on RP18 column using 1 % formic acid - methanol 19:1. Results obtained with either method were comparable.

J. Liq. Chromatogr. Relat. Technol. 33, 423-430 (2010). HPTLC of olmesartan and zidovidine on silica gel with ethyl acetate - methanol - acetic acid 160:40:1 in a twin-trough chamber saturated for 10 min. Quantitative determination by densitometric scanning at 269 nm. The linearity range was 80-600 ng/zone. LOQ was 80 ng/zone, the correlation coefficient 0.9900 and 0.9820. Recovery was 90.1 and 79.6 %. The accuracy and precision of the method were determined by repeatability (intra-day) and intermediate precision (inter-day) for the set of quality control samples (low, mid, high) in replicate. The results revealed excellent intra- and inter-day accuracy and precision of the method, which was within the acceptable limit (accuracy (% RE) 11.89 and 6.76 (low), 2.53 and 3.83 (mid), and 0.65 and 7.14 (high); inter-day precision (CV): 3.29 and 3.00 (low), 1.02 and 1.51 (mid), and 1.11 and 0.69 (high); intra-day precision: 2.59 and 2.86 (low), 1.07 and 1.13 (mid), and 1.04 and 0.72 (high) - after LLE and SPE, respectively).

J. AOAC Int. 93, 1222-1227 (2010). HPTLC of rosuvastatin (ROS) and ezetimibe (EZE) on silica gel with n-butyl-acetate - chloroform - glacial acetic acid 1:8:1 with chamber saturation for 30 min. Quantitative determination by densitometry at 245 nm. The hRf value of ROS was 30 and of EZE 58. The linearity for ROS and EZE was 0.1 to 0.9 µg/zone.The intraday and interday precision was 0.62 % and 0.73 % for ROS and 0.59 % and 0.69 % for EZE (n=3). The LOD values for ROS and EZE were found to be 0.04 and 0.07 µg/spot, respectively, and their LOQ values were 0.07 and 0.1 µg/spot, respectively. The recovery study results ranged from 98 to 101 % for ROS and EZE.

J. Liq. Chromatogr. Relat. Technol. 34, 2548-2464 (2011). HPTLC of tocopherol acetate (1) and tocopherol (2) in oral fluid vitamin E on silica gel with chloroform - cyclohexane 11:9. Quantitative determination by absorbance measurement at 202 nm for (1) and 272 nm for (2). The hRf values of (1) and (2) were 47 and 38, respectively. Linearity was in the range of 2-8 µg/band for (1) and (2). Limits of detection and quantification were found to be 50 and 150 ng/zone for (1). Precision was below 2 %. The intermediate/inter-day/intra-day precision was 0.4 % (n = 6). Recovery (by standard addition) was in the range between 99.8-101.5 %. Tocopherol acetate was better separated from tocopherol using normal phase TLC than by reversed phase TLC.

J. AOAC Int. 93, 904-910 (2010). HPTLC of imipramine hydrochloride and chlordiazepoxide on silica gel with carbon tetrachloride - acetone - triethylamine (pH 8.3) 20:10:1 in a twin-trough chamber after saturation for 30 min at 25 °C. Quantitative determination by densitometry in absorbance mode at 240 nm. Linearity was between 50-600 and 20-240 ng/zone with mean accuracies of 99.5 and 100.6 % for imiprazine hydrochloride and chlordiazepoxide, respectively. The hRf value of imipramine was 73 and of chlordiazepoxide 32. The %RSD values of intra-day and inter-day precision were between 0.7-1.4 % and 0.4-1.2 % for imipramine and 0.8-1.7 % and 0.7-1.3 % for chlordiazepoxide.

J. Liq. Chromatogr. Relat. Technol. 35, 280-293 (2012). HPTLC of darifenacin hydrobromide in bulk and in tablet dosage form on silica gel with toluene - acetone - methanol 3:1:1. Quantitative determination by absorbance measurement at 286 nm. The hRf value of darifenacin hydrobromide was 34 and selectivity regarding matrix was given. Linearity was between 50 and 450 ng/zone. The intermediate/inter-day/intra-day precision was below 1.4 % (n=6). The limits of detection and quantification were 30 and 91 ng/spot, respectively. Recovery (by standard addition) was between 98.8 and 100.8 %.

J. Planar Chromatogr. 25, 60-64 (2012). HPTLC of mebhydrolin napadisylate in tablets on silica gel with methanol - ethyl acetate 1:1. Quantitative determination by absorbance measurement at 287 nm. Linearity was in the range of 600-1600 ng/zone. Limits of detection and quantification were found to be 19 and 56 ng/zone, respectively. The intermediate/inter-day/intra-day precision was below 2 %. Recovery (by standard addition) was in the range of 99.3-100.8 %.

J. Planar Chromatogr. 26, 78-85 (2013). HPTLC of lamivudine (1), tenofovir disoproxil fumarate (2) and efavirenz (3) on silica gel with chloroform - methanol - toluene 30:4:1. Quantitative determination by absorbance measurement at 260 nm. The hRf values of (1), (2) and (3) were 20, 61 and 73, respectively. Linearity was in the range of 400-800 ng/zone for (1) and (2) and 800-1600 ng/zone for (3). Intermediate precision was below 2 %. LOD and LOQ were 180 and 300 ng/zone for (1), 150 and 210 ng/zone for (2) and 300 and 400 ng/zone for (3), respectively.