Indian Drugs 44 (3), 205-208 (2007). HPTLC of frusemide (= furosemide) and spironolactone on silica gel with toluene - acetonitrile - glacial acetic acid 70:30:2, with chamber saturation for 15 min at room temperature. Development over 8 cm, followed by air drying. Quantitative determination by densitometry at 254 nm. Linearity was between 8 - 32 ng/µL and 20 - 80 ng/µL for frusemide and spironolactone respectively. The method was validated for accuracy and precision. The limit of detection and quantification for frusemide was 3 ng/µL and 8 ng/µL respectively, and for spironolactone 2 ng/µL and 6 ng/µL, respectively. Recovery by standard addition was 99.4-101% for both compounds.

(Isolation and identification of the metabolites of 5-ethyl-2'-deoxyuridine from rat urine.) Drug Res. 35, 1055-1057 (1985). Separation of the metabolites of ethyl-deoxyuridine (5-(1-hydroxethyl)uracil; 5-ethyluracil) on silica with chloroform - isopropanol 8:2 and 5:5. Detection by UV 254 nm.

J. Chinese Herb Med. 18, 405-408 (1987) (Zhong Caoyao). TLC of 3H-Rg1 on silica with chloroform - methanol - water 65:35:10. Detection by spraying with 5% sulfuric acid - methanol and heating at 100-110 °C for 5 min. Quantification by scintillation counting after elution.

Chromatographia 29, 44-50 (1990). HPTLC of azidothymidine and its degradation product thymine on silica with a) methanol - chloroform 5:95, 7:93, 10:90, 15:85, b) butanol - heptane - acetone - NH3 26% 4:3:3:1. Quantification by densitometry at 268 nm. The calibration curve for HPTLC was linear over the range of 25 - 100 ng thymine/spot. Also, reversed-phase HPLC.

J. Planar Chromatogr. 5, 255-258 (1992). TLC of antipyrine, norantipyrine, 4-hydroxyantipyrine, dimethylaminoamtipyrine and 3-hydroxymethylantipyrine on silica with chloroform - methanol - trifluoroacetic acid 95:5:1. Detection and quantification under UV 254 nm and by scanning densitometry. Spectra of the analytes were obtained directly from the silica gel using FABMS-MS (fast atom bombardment mass spectrometry and tandem mass spectrometry).

Arzneim.-Forsch./Drug Res. 46, 106-113 (1996). TLC separation of montirelin hydrate ((-)-N-[[(3R,6R)-6-methyl-5-oxo-3-thiomorpholinyl]-carbonyl]-L-histidyl-L-prolinamide tetrahydrate) and its degradation product on silica with ethanol - 0.1 N hydrochloric acid 10:3 and chloroform - ethanol - 28 % NH3 - water 10:10:1:1. Detection under UV 254 nm and autoradiography.

Chinese J. Modern App. Pharm. (Zhangguo Xiandai Yingyong Yaoxue Zazhi) 18 (4), 284-287 (2001). TLC on silica gel with 1) chloroform - acetone 19:1, 2) butanol - acetic acid - water 4:4:1. Detection 1) by spraying with 10% phosphomolybdic acid and heating at 110°C for 10 min, 2) by spraying with 10% sulfuric acid and heating at 110°C for 10 min and under UV 365 nm, 3) under UV 365 nm, 4) by spraying with 0.2% ninhydrin in ethanol and heating at 105°C for 10 min. Identification by finger print technique.

Chromatographia 67 (1-2), 101-107 (2008). HPTLC of simvastatin and ezetimibe on silica gel with n-hexane – acetone 3:2. Quantification by densitometry in absorbance mode at 234 nm. The hRf value of simvastatin was 39 and of ezetimibe 50. Linearity was between 200 and 1600 ng/spot with correlation coefficients r2 = 0.9917 for simvastatin and r2 = 0.9927 for ezetimibe. Limits of detection and quantitation were 25 and 150 ng per band, respectively. For investigation of stability simvastatin and ezetimibe were subjected to acid, pH 6.8 phosphate buffer, oxidation, dry heat, and wet heat. The degradation products were well resolved from the pure drug, therefore the method could be effectively used for stability-indicating analysis.