J. Planar Chromatogr. 26, 421-426 (2013). HPTLC of umbelliferone in the leaves of eleven Juniperus species on silica gel with n-hexane - ethyl acetate 7:3 in the first run and dichloromethane - diethyl ether 4:1 in the second run. Quantitative determination by absorbance measurement at 326 nm. The hRf of umbelliferone was 30. Linearity was between 10 and 80 ng/zone. LOD and LOQ were 7 and 23 ng/zone. Average recovery (by standard addition) was 96.3 %. Intermediate intra- and inter-day precision (n = 6) was below 7 %.

Chinese J, Mod. Drug Appl. 6 (22), 129-130 (2012). Huaxue Jietonggao ointment is herbal TCM preparation for the treatment of sprain, contusion, rheumatism, lumbago and back ache. For quality control, TLC on silica gel 1) for Capsicum annuum and the standard capsaicin, with petroleum ether (30-60 °C) – ethanol 9:1, detection under UV 254 nm; 2) for Zingiber officinale Rosc., with petroleum ether (60-90 °C) – ethyl acetate 2:1, detection by spraying with 2 % p-dimethylaminobenzaldehyde in 40 % sulfuric acid in ethanol and heating at 105 °C until the zones are visible in daylight; 3) for Heracleum hemsleyanum, with n-hexane – toluene – ethyl acetate 2:1:1, detection under UV 366 nm.

Chinese J. of Inform. on TCM 19 (6), 57-59 (2012). In TCM Tongfu Keli granule is prescribed for postoperative gastrointestinal function recovery, reducing inflammatory intestinal obstruction, and intestinal adhesion formation. For quality control, TLC on silica gel 1) for Rheum palmatum L., with petroleum ether (30-60 °C) – ethyl formate – formic acid 15:5:1, detection under UV 366 nm; 2) for immature Bitter Orange, with toluene – ethyl acetate – formic acid – water 20:10:1:1, detection by spraying with 3 % aluminium chloride in ethanol and viewing under UV 366 nm; 3) for Magnolia officinalis Rehd. et Wils., with cyclohexane – acetone 5:2, detection under UV 254 nm. Quantification of aloe-emodin, rhein, emodin, chrysophanol, physcion by HPLC.

CBS 111, 13-15 (2013). HPTLC of propolis on silica gel in a twin trough chamber with 8 mL of n-hexane – ethyl acetate – glacial acetic acid 5:3:1 and preconditioning with 5 mL hydrochloric acid (37 %) in the opposite trough using filter paper wetted with the hydrochloric acid shortly before chromatography. Migration distance was 65 mm. Detection under UV 366 nm after dipping in 0.5 % methanolic 2-aminoethyldiphenylborane reagent followed by drying and dipping in 5 % methanolic polyethylene glycol solution. Different types of German propolis were found, an orange, a blue and mixed types. Comparison with HPTLC fingerprints of the possible origin plants showed that the orange type most likely derives from Populus nigra. The blue type correlated to a certain extent with Populus tremula as well as both types with Aesculus hippocastanum L. Confirmed by MS the following substances were found in the orange type: pinocembrin, galangin, caffeic acid phenethylester and chrysin. The blue type contained p-cumaric acid and ellagic acid. Caffeic acid, quercetin and apigenin appeared in both types.

Chinese J. of Inform. on TCM 20 (8), 56-58 (2013). Zaodi Anshen Wan pill is herbal TCM for treatment of neurasthenia, fatigue syndrome, depression, insomnia, dizziness and tinnitus, palpitation, etc. For quality control, HPTLC on silica gel 1) for Ziziphus jujuba Mill. var. spinosa, with water-saturated n-butanol, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4, evaluation under UV 366 nm; 2) for Radix polygoni multiflori preparata and the standards emodin and physcion, with petroleum ether (30-60 ˚C) – ethyl formate – formic acid 15:5:1, detection by exposure to ammonia vapors, evaluation under UV 366 nm. Analysis of Schisandra chinensis, schisandrin, deoxyschizandrin and gamma-schizandrin B by HPLC.

J. Planar Chromatogr. 28, 61-66 (2015). HPTLC of swertiamarin in extracts of Enicostemma littorale and Swertia chirata on silica gel with ethyl acetate – methanol – water 16:3:1. Quantitative determination by absorbance measurement at 246 nm. The hRF value of swertiamarin was 58. Linearity was between 200 and 1200 ng/zone. The intermediate intra-day and inter-day precisions were below 2 % (n=6). The LOD and LOQ were 38 and 115 ng/zone, respectively. Recoveries were in the range of 98-100 %.

Guide Chinese Med. 17, 85-87 (2014). Zhituo Shenfa Wan pills are a TCM preparation or the treatment of seborrheic alopecia and Alopecia areata. For quality control, TLC on silica gel 1) for Angelica sinensis and Ligusticum chuanxiong Hort, with n-hexane – ethyl acetate 19:1, detection at UV 366 nm; 2) for Atractylodes lancea (Thunb.) DC., with petroleum ether (60-90 °C) – ethyl acetate 19:1, detection by spraying with 5 % p-dimethylaminobenzaldehyde in sulfuric acid – ethanol 1:4 and evaluation under white light; 3) for Corydalis yanhusuo W. T. Wang ex Z. Y. Su et C. Y. Wu and the standard tetrahydropalmatine, with n-hexane – chloroform – methanol – triethylamine 10:6:1:1, detection by exposure to iodine vapors until the zones are clearly visualized, evaluation under white and at UV 366 nm; (4) for Pericarpium Citri reticulatae and the standard hesperidin, with chloroform – acetone – methanol 5:1:1, detection by spraying with 3 % aluminium trichloride in ethanol and heating mildly until the zones are visible under white and at UV 366 nm. Quantification of hesperidin by HPLC.

Pharm. Clinics Chinese Materia Med. 4 (6), 21-23 (2013). Lishen Jiaonang capsule is a TCM preparation for treatment of renal diseases. For quality control, TLC on silica gel 1) for Equisetum hyemale L. and the standard kaempferide with cyclohexane – ethyl acetate – formic acid 20:10:1, detection by spraying with 5 % aluminum trichloride in ethanol and evaluation at UV 366 nm; 2) for Heartleaf Houttuynia Herb with benzene – chloroform – acetone 8:5:7, detection at UV 366 nm. Quantification of kaempferide by HPLC.