Chinese J. Hosp. Pharm. 29 (4), 1246-1247 (2009). TLC of TCM drug extracts on silica gel with 1) chloroform - methanol - acetone 10:1:1; 2) toluene - chloroform - acetone - methanol - formic acid 4:6:8:1:4; 3) chloroform - methanol 4:1. Detection 1) after exposure to ammonia vapor under UV 254 nm; 2) under UV 254 nm; 3) by spraying with 10 % sulfuric acid in ethanol followed by heating at 105 °C until coloration.

J. AOAC Int. 92, 1343-1348 (2009). HPTLC of beta-sitosterol, beta-amyrin and plant extracts on silica gel with chloroform - n-hexane - methanol 13:6:1. Detection by spraying with vanillin-orthophosphoric acid reagent, 5 % phosphomolybdic acid, or anisaldehyde reagent, followed by heating at 110 °C for 5 min. Vanillin reagent provided the best results. Quantitative determination by absorbance measurement at 525 nm.

International Seminar on Herbal Drug Research, PN-009 (2009). Bioassay guided fractionation of Lagenaria siceraria was carried out on a silica gel column with solvents in ascending order of polarity. Each fraction obtained was subjected to preparative TLC on silica gel with n-butanol - methanol - water 3:1:1. Four bands with different Rf values were collected and active compounds were extracted and screened for antihyperlipidemic activity.

J. Planar Chromatogr. 23, 225-226 (2010). OPLC of oregano oil components (carvacrol, thymol, and linalool) on silica gel with dichloromethane. Detection under UV 254 nm, by spraying with vanillin-sulfuric acid reagent (0.1 g vanillin, 100 mL ethanol, and 2.2 mL 95-98 % sulfuric acid) and heating at 110 °C for 3 min, and in the BioArena system (the dried developed plates were dipped for 10 s into an aqueous cell suspension of the soil bacteria Bacillus subtilis and incubated for 2 h at 100 % rel. humidity and 30 °C). Visualizion of antimicrobial compounds was performed by immersing the plates for 5 s in an aqueous solution of MTT reagent (80 mg MTT and 100 mg Triton X-100 in 100 mL water). The layers were further incubated and and documented.

Abstract No. C-291, 61st IPC (2009). Quantitative determination of curcumin in the marketed herbal product Rheumax (Herbajules rumatis) containing extracts of Curcuma longa, Boswellia serrata, Tinospora cordifolia and Vitex negundo by TLC on silica gel with chloroform - methanol 37:3. The hRf value of curcumin was 59. Quantitative absorbance measurement at 430 nm. The linear regression between 100 and 500 ng/zone showed R2 of 0.99984 and RSD of 1.58 % for curcumin.

Phytochem. Anal. 21, 556-560 (2010). HPTLC of betulinic acid in the rhizomes of Nelumbo nucifera on silica gel with chloroform - methanol - formic acid 49:1:1. Detection by spraying with anisaldehyde - acetic acid - methanol - sulfuric acid 5:100:850:50 and drying at 50 °C for 10 min. Quantitative determination by absorbance measurement at 420 nm. The hRf of betulinic acid was 30. Linearity was between 2 and 10 µg/zone. Detection and quantification limits were 0.4 and 2.30 µg, respectively. The intra-day and inter-day precisions were 0.4 % and 0.3 % (n=3) respectively. Recovery (by standard addition) was 98.4 %.

J. Chinese Modern Med. & Pharm. 17 (34), 52-53 (2010). TLC on silica gel with chloroform – ethyl acetate – methanol – formic acid 2000:25:50:1. Detection under UV 366 nm. Identification of rhaponitin in both medicines by comparison with the standard.

Yunan J. of Chinese Trad. Med. & Pharm. 31(10), 58-60 (2010). TLC of extracts of Cishushi suppository: 1) for Panax notoginseng, on silica gel with chloroform – methanol – water 14:6:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ºC; 2) for Fructus Cnidii, on silica gel with petroleum ether (60-90 °C) – ethyl acetate 7:3, detection under UV 366 nm; 3) for Borneolum Syntheticum, on silica gel with toluene – ethyl acetate 19:1, detection by spraying with 5 % vanillin-sulfuric acid reagent and heating at 105 °C; 4) for Fructus Sophorae, on silica gel with chloroform – methanol – water – formic acid 700:300:50:1, detection by spraying with 1 % AlCl3 in ethanol, heating and evaluation under UV 366 nm.