J. of Medicinal Chemistry 32, 2432- 3435 (1989). TLC of vasotocin analogues on silica with butanol - pyridine - acetone water 15:10:3:6 or 15:10:3:12 and butanol acetic acid - water 4:1:1. Detection by exposure to chlorine followed by spraying with 1% aqueous KI-starch solution.

Proc. 6th Int. Symp. Instrum. Planar Chromatogr., (Interlaken 1991), Inst. Chromatogr., Bad Dürkheim, FRG, 401-408 (1991). Preliminary results are described for the combination of off-line TLC-FAB-MS for polar, ionisable compounds and their conjugated metabolites. HPTLC of benzoic acid, hippuric acid, phenolphthalein and its gucuronide, nitrophenol and its glucuronide and paracetamol and its sulfate on silica with chloroform - ethanol 3:2. Separated substances were removed and determinated by FAB-MS and MS-MS.

Arzneim.-Forsch./Drug Res. 45, 766-770 (1995). TLC of efonidipine hydrochloride (2-benzyl(phenyl)amino)ethyl 1,4-dihydro-2,6-dimethyl-5-(5,5-dimethyl-2-oxo-1,3,2-dioxaphosphorinan-2-yl)-4-(3-nitrophenyl)-3-pyridinecarboxylate hydrochloride ethanol) and metabolites on silica with chloroform - methanol - diethylamine 18:1:1 or chloroform - methanol 1:1. Detection under UV 254 nm.

J. Planar Chromatogr. 11, 394-399 (1998). A new simple and powerful technique, on-line sample collection after high-resolution step-wise gradient OPLC separation combined with digital autoradiography (DAR) has been used for isolation of plasma and urine metabolites of 3H- or 14C-radiolabeled deramciclane (a new anxiolytic compound). This highly efficient separation can be followed by different MS techniques (FAB-MS, FAB-MS-MS, HPLC-MS, and HPLC-MS-MS) for determination of the structures of minor and major metabolites in different biological matrices. The combination of these methods resulted in a simple, sensitive, and efficient technique for metabolite research. OPLC on HPTLC silica gel, prewashed with methanol - water 4:1 with A) chloroform - acetonitrile 3:2 and B) n-butanol - acetic acid - water 4:1:1 for the gradient. Detection by digital autoradiography.

59th Indian Pharmaceutical congress F-225, 443, (2007). HPTLC cinnamaldehyde in the bark powder of Cinnamomum zeylenicum on silica gel with toluene - ethyl acetate - formic acid 190:10:1. Densitometric evaluation at 295 nm for quantification. The method was linear within the range of 31 and 157 ng/zone. The identity of the compound was confirmed by over overlaying the UV spectra of sample and standard. Cinnamomum bark was found to contain 0.25 % of cinnamaldehyde. Limit of detection and quantification was 3000 and 9900 ng/mL, respectively.

Chromatographia 70 (1-2), 309-313 (2009). TLC on silica gel with acetonitrile - methanol - dichloromethane 2:1:2. The hRf value of dutasteride was 64. Separation of dutasteride from its degradation products (produced by acid and alkali hydrolysis, oxidation, photo degradation, dry and wet heat treatment) was good. Quantitative determination by absorbance measurement at 244 nm. Linearity was in the range of 100 - 600 ng/band and the correlation coefficient was 0.9943 (via peak area) The limit of detection and quantitation was 7 and 23 ng/band.

J. Chinese Trad. Patent Med. 30 (12), Supl. 4-6 (2008). TLC of the TCM drug extracts on silica gel with 1) dichloromethane - ethyl acetate - methanol - water 15:40:22:10; 2) petroleum ether (60-90 °C) - diethyl ether 3:2; 3) petroleum ether (60-90 °C) - ethyl acetate 25:2; 4) n-butanol - acetic acid - water 4:1:2; 5) chloroform - diethyl ether 1:1. Detection 1) by spraying with 10 % sulfuric acid in ethanol followed by heating at 105 °C until coloration; 2) under UV 365 nm; 3) under UV 254 nm.

Talanta, 80 (5), 2007-2015 (2010). Presentation of a method for determination of oxybutynin hydrochloride (OX) in presence of its degradation product and additives in its pharmaceutical formulations. UV spectrophotometry using the first derivative of ratio spectra and measurment at 216 nm. Chemometric analysis using principal component regression and partial least-squares. HPTLC of OX and its degradation products methylparaben and propylparaben on silica gel with chloroform - methanol - ammonia - triethylamine 500:15:2.5:1. Quantitative determination by densitometry at 220 nm. Comparison of the results obtained with all three methods showed no significant differences.