Mitt. Mechanismus der Dilactam-Bildung. / Indole cleavage with mebhydroline by sodium periodate – Part 2. Mechanism of the dilactam formation (German). Pharmazie 61, 391-399 (2006). TLC of 5-benzyl-2,3,4,5-tetrahydro-2-dimethyl-1H-pyrido[4,3-b]indol-2-ium-bromid, 2,5-dimethyl-2,3,4,5-tetrahydro-1H-pyrido[4,3-b]indol and 15 other compounds on alumina with chloroform - ethanol- ammonia 13:6:1; and benzene - ethyl acetate 3:2. Also TLC on silica gel with chloroform - acetone - ethanol - ammonia 90:10:10:1, diisopropyl ether, and chloroform - ethyl acetate 7:3. Detection under UV light at 254 and 366 nm, Dragendorff reagent (and spraying with 10% sulfuric acid), and Ehrlich reagent.

Drug. Res. 35, 1542-1544 (1985). Determination of dimethylamino hydroxypropoxy triamterene on silica with chloroform - methanol 7:3 in a solvent-saturated glass tank with NH3-atmosphere. Quantification by measuring the fluorescence at 365 nm. Detection limit 5 ng/ml standard.

1. Direct analysis of conjugates by thin-layer chromatography-secondary-ion mass spectrometry. J. Chromatogr. 414, 139-148 (1987). Description of a method for direct molecular-secondary-ion mass spectrometry measurement of conjugates of p-nitrophenol and 4-hydroxyanti-pyrine following TLC on silica with butanol - acetic acid - ethanol - water 3:1:1:1. Discussion of the quantitative application using deuterium-labelled internal standards for p-nitrophenol conjugates.

Arzneim.-Forsch. 40, 311-316 (1990). HPTLC of etofenamate and metabolites on silica with cyclohexane – ethyl acetate – formic acid 16:8:1. Quantification by fluorodensitometry at 366 nm after spraying with formaldehyde solution (ethanol – 37% formaldehyde in water – conc. formic acid 5:4:1) and dipping into paraffin.

Arzneim.-Forschung / Drug Res. 44, 527-538 (1994). TLC of ebastine (4'-tert-butyl-4-(4-(diphenylmethoxy)piperidino)butyrophenone), carebastine and metabolites on silica with chloroform - methanol - ethyl acetate - acetic acid 78:10:10:2. Radio scanning.

A newly developed rapid tool for studying drug metabolism. J. Planar Chromatogr. 10, 399-405 (1997). Comparison of advantages and disadvantages of combined thin-layer chromatography - digital autoradiography (TLC-DAR) and other techniques used for plate evaluation in planar chromatography. It is demonstrated that this newly developed method can be widely used for the analysis of a variety of biological samples and, because information is obtained quickly at low cost, it is an excellent tool in metabolism research. DAR is a convenient and extremely rapid means of locating and quantifying radioactive metabolites on the TLC plate after separation and/or purification. Example used: TLC on silica of deramciclane (a new anxiolytic compound) and metabolites with butanol - acetic acid - water 4:1:1.

JAOAC Int. 84, 1252-1257 (2001). Analytical TLC of celiprolol (3-{3-acetyl-4-[3-(tert-butylamino)-2-hydroxypropoxy]phenyl}-1,1-diethyl urea) on silica gel with ethyl acetate - methanol - strong NH3 17:2:1. Detection and quantification at 243 nm. Detection limit was 10 ng/mL.

J. Sep. Sci. 30, 2167-2172 (2007). HPTLC of clozapine in human serum on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 290 nm. Linearity was between 10 and 100 ng/zone. The intra-assay variation was between 2.10 and 3.33 % (n=5) and inter-assay variation was between 2.67 and 4.44 % (n=9). The limits of detection and quantification were 0.03 and 0.05 ng/µL, respectively. Recovery was between 97.0 and 99.0 %, and selectivity regarding matrix was given.