CBS 93, 2-4 (2004). HPTLC of signaling ceramides on silica gel with a 7 step gradient from methanol over dichloromethane to n-hexane over 42 min. Detection by dipping in manganese chloride reagent for 1 s, followed by drying at 120 °C for 20 min. Quantitative determination by absorbance measurement at 550 nm and Michaelis-Menten regression 2 via peak area. Signaling ceramides are separated from other lipids (shingomyelin, phosphatidylcholine, cholesterol) contained in cellular lipid extracts. Comparison with determination of ceramide formation via isotope labeled standards and conventional TLC method.

Abstract No. F-283, 61st (2009). HPTLC of naproxen sodium on silica gel with toluene - ethyl acetate - acetic acid 15:3:2. The hRf value was 63. Quantitative determination by absorbance measurement at 230 nm. The method was linear in the range of 100-500 ng/band.

Ind. J. Pharma. Sci. 71(4), 451-454 (2009). HPTLC of clotrimazole in bulk drug and tablet dosage form on silica gel with cyclo hexane - toluene - methanol - triethyl amine 80:20:5:2. Quantitative determination by absorbance measurement at 262 nm. The calibration curve was linear between 200 to 1000 ng/spot for clotrimazole. The limit of detection and limit of quantification for clotrimazole were 50 ng/spot and 200 ng/spot, respectively.

Abstract No. F-263, 61st IPC (2009). HPTLC of repaglinide (REPA) and rosiglitazone (ROSI) on silica gel with benzene - methanol - acetone - acetic acid 80:10:9:1. Quantitative determination by absorbance measurement at 266 nm. Lienarity was in the range of 800-2800 ng/band and 400-2400 ng/band for REPA and ROSI, respectively. Recovery was 101.7 and 99.5 % for REPA and ROSI, respectively.

J. Planar Chromatogr. 22, 399-403 (2009). TLC of celecoxib, etoricoxib, and valdecoxib on silica gel with chloroform - acetone - toluene 12:5:2 with chamber saturation for 15 min at room temperature. Quantitative determination by absorbance measurement at 254 and 290 nm.

J. Planar Chromatogr. 23, 134-136 (2010). TLC of sertraline on silica gel (prewashed with methanol) with chloroform - ethyl acetate - triethylamine 25:15:1 in a twin-trough chamber previously saturated for 10 min at room temperature. Quantitative determination by absorbance measurement at 279 nm. The hRf for sertraline was 40. Intra-day precision and inter-day precision was 99.84 % RSD and 99.92 % RSD, respectively. A good linear relationship between response (peak area) and amount was obtained over the range 2.7-7.9 µg/band.

J. Planar Chromatogr. 23, 119-122 (2010). HPTLC of artemether and lumefantrine on silica gel with n-hexane - ethyl acetate 4:1 at room temperature in a twin-trough chamber saturated for 15 min. Quantitative determination by densitometric scanning in reflectance mode at 357 nm. The method is linear (r2 > 0.995), precise (RSD < 2 %), accurate (average recovery of 100.5 % for artemether and 99.5 % for lumefantrine), specific, and robust. LOD and LOQ for artemether were 50 and 150 ng/band, respectively, and those for lumefantrine were 300 and 900 ng/band, respectively.

Asian Journal of Chemistry 22(6), 4209-4213 (2010). HPTLC of beta-blockers on silica gel with toluene - ethyl acetate - acetone - 25 % ammonia 10:20:20:1. The method was applied to biological samples (urine and blood). The drugs were extracted from biological samples by liquid-liquid extraction. Detection at 254 nm. The corresponding separated spots were scraped from the plate, extracted with methanol and analyzed by mass spectrometry. Finally the identity was confirmed by comparing the spectra with a data library. The proposed HPTLC method coupled with MS is very sensitive and highly suitable for biological samples.