f. ) Etting, (Loranthaceae), growing on different substrates. J. Planar Chromatogr. 24, 60-65 (2011). HPTLC of phenolic compounds with caffeic acid, (+)-epicatechin, ellagic acid, gallic acid, and kaempferol as markers on silica gel with toluene - ethyl acetate - methanol - formic acid 14:6:1:1 in a twin-trough chamber with saturation for 30 min at 24 °C. Quantitative determination by absorbance measurement at 300 nm. Detection by dipping in anisaldehyde-sulfuric acid reagent followed by heating at 110 °C for 5 min. Evaluation under UV 254 nm and visible light after derivatization. Repeatability (n = 7) was between 0.5-2.4 %; intermediate precision was between 1.5-4.4 %. For (+)-epicatechin, ellagic acid, gallic acid, caffeic acid, and kaempferol, LOD was 332, 225, 21, 64, and 35 ng/zone, LOQ was 1157, 740, 67, 242, and 115 ng/zone, and precision (%RSD) was 3.8, 4.9, 4.7, 5.4, 1.7 %, respectively. The hRf value was 39 for (+)-epicatechin, 43 for ellagic acid, 55 for gallic acid, 65 for caffeic acid, and 72 for kaempferol.

J. Liq. Chromatogr. Relat. Technol. 32, 2437-2450 (2009). HPTLC calliterpenone (1) and calliterpenone monoacetate (2) in the leaves of Callicarpa macrophylla on silica gel with methanol - water 9:11. Quantitative determination by absorbance measurement at 210 nm. The hRf values of (1) and (2) were 43 and 73, respectively. Linearity was between 1-5 µg/zone for (1) and (2). LOD and LOQ were 230 and 780 ng/zone for (1) and 220 and 730 ng/zone for (2). Intra-day and inter-day precisions ranged between 1.3-1.7 % for (1) and 1.1-1.7 % for (2). Recoveries (by standard addition) were between 97.5-100.8 % for both.

J. Liq. Chromatogr. Relat. Technol. 35, 1141-1145 (2012). HPTLC of diosgenin in various parts of Tribulus terrestris L. on silica gel with toluene - ethyl acetate - methanol 7:3:1. Detection by dipping in anisaldehyde reagent consisting of anisaldehyde - acetic acid - sulfuric acid - methanol 1:20:10:170, followed by heating at 110 °C for 2 min. Quantitative determination by absorbance measurement at 430 nm. The hRf value of diosgenin was 48 and selectivity regarding matrix was given. Linearity was between 50 and 240 ng/zone. The method provides acceptable intra-day and inter-day precision for diosgenin. The limits of detection and quantification were 2 and 7 ng/spot, respectively. Recovery (by standard addition) was 100.6 %. The method provided comparable results with HPLC.

and quantitative determination of gallic acid in Zhuqi Fugan granules) (Chinese). Chinese J. of Inform. on TCM 19 (4), 47-48 (2012). Zhuqi Fugan granules, a herbal TCM preparation, are effective for clearing heat and removing toxicity, nourishing the liver, and strengthening the body resistance and are clinically prescribed to cure viral hepatitis type B. For quality control, TLC of the extracts of the preparations on silica gel with cyclohexane – chloroform – ethyl acetate 4:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 ° until the zones are visible.