Phytochem. Anal. 19, 244-250 (2007). HPTLC of taraxerol in Clitoria ternatea on silica gel with hexane - ethyl acetate 4:1. Detection by spraying with anisaldehyde reagent. Quantitative determination by absorbance measurement at 420 nm. The hRf value of taraxerol was 53 and selectivity regarding the matrix was given. Linearity was between 100 and 1200 ng of taraxerol. Recovery values were between 99.6 and 99.7 %. The limit of detection and quantification for taraxerol was 31 and 105 ng/spot, respectively.

heracleifolia, C. dahurica, or C. americana. J. AOAC Int. 91, 1257-1264 (2008). HPTLC of black cohosh extracts and references cimifugin, 23-epi-26-deoxyactein, actein on silica gel with toluene - ethyl formate - formic acid 5:3:2 in a twin-trough chamber saturated for 20 min. HPTLC plates were conditioned to 5 % relative humidity or less. For identification of species derivatization by dipping for 1 s in sulfuric acid reagent (10 % in methanol) followed by heating at 100 °C for 5 min and evaluation under white light and UV 366 nm. For detection of adulteration with Cimicifuga foetida derivatization with boric acid/oxalic acid reagent, for detection of adulteration with C. heracleifolia and C. dahurica derivatization with antimony(III) chloride reagent. Evaluation under UV 254 nm and after derivatization under UV 366 nm and white light. The method allows visual detection of 5 % of these adulterants in Cimicifuga racemosa.

Phytochem. Anal. 20, 177-190 (2009). TLC and HPTLC of polyphenols (tellimagrandins I and II, rugosins A, B, D and E and other) in commercially available products of meadowsweet (Filipendula ulmaria) and dog rose (Rosa canina) on silica gel, LiChrospher Si 60, RP-18 and amino phase with tetrahydrofuran - acetonitrile - water 3:1:1, of tannins on amino phase with acetone - formic acid 3:1, of flavonols with acetone - formic acid 17:3, of tannins with diisopropyl ether - acetone - formic acid - water 4:4:1:1, and of flavonols with diisopropyl ether - acetone - formic acid - water 5:3:1:1. Detection of polyphenolic compounds under UV light at 254 and 366 nm before and after spraying with natural products reagent followed by polyethylene glycol reagent. Detection in white light after spraying with 1 % iron(III) chloride (for tannins and phenolic acids), vanillin-hydrochloric acid (flavonols) or bis-diazotised sulfanilamide (for all polyphenols). Quantitative determination of polyphenol contents by HPLC with UV detection. Meadowsweet flowers and rose hips with seeds yielded 55.5-124.8 and 0.4-1.3 mg/g of ellagitannins, respectively. The sum of detected polyphenols was 83.9-165.7 mg/g for Filipendulae ulmariae flos and 1.2-2.7 mg/g for Rosae pseudo-fructus cum fructibus.

Abstract No. 9148, IHCB (2009). HPTLC of gallic acid in Emblica officinalis (an important constituent of Triphala, a popular ayurvedic formulation) on silica gel with toluene - ethyl acetate - formic acid 10:10:3. The hRf value of gallic acid was 45. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the range of 150-530 ng/spot. The extract was found to contain 7.5 mg/g of gallic acid.

J. Agric. Food Chem. 56, 7644-7648 (2008). HPTLC of ferulic acid from agricultural waste (maize bran, rice bran, wheat bran, wheat straw, sugar cane baggasse, pineapple peels, orange peels and pomegranate peels) on silica gel with benzene - dioxane - acetic acid 85:15:1. Detection under UV 254 nm. Purification by adsorption chromatography followed by preparative layer chromatography.

J. AOAC Int. 92, 1021-1026 (2009). HPTLC of catechin and extracts from polyherbal oil formulations on silica gel using chloroform - acetone - 0.1 % formic acid 77:15:8 % in a twin trough chamber saturated for 20 min. Quantitative determination by absorbance measurement at 296 nm. The limit of detection and quantification was 6 and 20 ng/spot, respectively.

Pharmazie 64, 104-109 (2009). TLC of christinin-A as marker and leaf extracts on silica gel with chloroform - methanol - water 13:8:2 and butanol - acetic acid - water 4:1:5 (upper phase). Detection by spraying with p-anisaldehyde reagent.

J. Planar Chromatogr. 22, 301-304 (2009). Analytical and preparative TLC of luteolin-7-rutinoside and luteolin-7-neohesperidoside on silica gel with ethyl acetate - formic acid - acetic acid - water 100:11:11:23, on cellulose with 30 % acetic acid, and on polyamide with chloroform - methanol - 2-butanone - acetyl acetone (= pentane-2,4-dione) 9:4:3:1. Detection by spraying with natural products reagent followed by treatment with PEG 4000, or by aniline phthalate.