Phytochem. Anal. 19, 116-121 (2008). HPTLC of cinnamaldehyde (1) in the shoots of Cinnamomum zeylanicum, eugenol (2) in the flower buds of Eugenia caryophyllus and piperine (3) in the fruits of Piper nigrum on silica gel with petroleum ether - dichloromethane - formic acid 20:40:1. Quantitative determination by absorbance measurement at 290 nm. The hRf values were 47, 61, and 12 for (1), (2), and (3), respectively. Linearity was between 54 and 735 ng/spot for (1), 533 and 8531 ng/spot for (2), and 50 and 300 ng/spot for (3). The limits of detection and quantification were 12 and 21 ng/spot for (1), 240 and 426 ng/spot for (2), and 18 and 40 ng/spot for (3). Recovery was 99 % for each substance. No significant intra- and interday variation was observed. The method proved to be rapid and useful in comparison with GC and HPLC methods.

J. Planar Chromatogr. 21, 21-26 (2008). HPTLC of extracts of Hoodia gordonii with fructose and beta-sitosterol as standards on silica gel with chloroform - methanol - water 70:30:3 in an automatic developing chamber fitted with a twin-trough chamber. The chamber was saturated for 20 min with mobile phase and relative humidity was controlled (33 %). Detection by dipping in anisaldehyde reagent, followed by heating at 100 °C for 3 min. Documentation and evaluation before derivatization under UV 366 nm and after derivatization under white light. The method was validated. It is specific and allows discrimination of Hoodia gordonii from Hoodia currorii, Hoodia parviflora, and the common adulterant prickly pear cactus (Opuntia ficus-indica). The sample is stable in solution and on the plate for at least 3 h, as well as during chromatography (2D test). After derivatization the chromatogram is stable for at least 1 hour. Precision (repeatability, intermediate precision, and reproducibility was good and the method is robust . The method is sensitive to changes in relative humidity. If relative humidity exceeds 47% the plate must be conditioned to 33% RH to ensure proper separation.

Abstract No. 9147, IHCB (2009). HPTLC of quercetin in methanolic leaf extracts of Viola odorata on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:26. Quantitative determination by absorbance measurement at 366/>400 nm for quantification. The extract contained 0.36 % quercetin.

Phytochem. Anal. 20, 154-158 (2009). TLC of the purgative constituents of rhubarb (sennoside A and sennoside B) on silica gel with 1-propanol - ethyl acetate - water - acetic acid 40:40:30:1. After drying detection by spraying with 10 % sulfuric acid and heating. For the eastern blotting assay the developed TLC plate was dried and sprayed with isopropanol - methanol - water 1:4:16. Transfer by using a PVDF membrane sheet for further treatment.

J. Planar Chromatogr. 22, 109-113 (2009). HPTLC of purpurin on silica gel with toluene - ethyl acetate - formic acid 98:2:1 in a twin trough chamber saturated for 20 min at 25 +/- 2 °C. Quantitative determination by absorbance measurement at 255 nm. The limit of detection and quantification was 50 and 100 ng/band, respectively.

J. Planar Chromatogr. 22, 445-448 (2009). HPTLC of myristicin, safrole and extract of seeds on silica gel, prewashed with methanol, with toluene in an automatic developing chamber saturated for 20 min. Quantitative determination by absorbance measurement at 210 nm for myristicin and at 290 nm for safrole.

J. Chinese Trad. & Herb. Drugs 40 (8), 1249-1252 (2009). TLC of extracts of the TCM drug on silica gel with 1) methanol - acetic acid - water 18:1:4; 2) petroleum ether (60-90 °C) - ethyl acetate 1:1; 3) toluene - ethyl acetate - methanol 5:5:3; 4) petroleum ether (60-90 °C) - ethyl acetate - formic acid 15:5:1. Detection 1) by spraying with potassium iodobismuthate reagent; 2) under UV 254 nm; 3) by spraying with 5 % AlCl3 in ethanol and evaluation under UV 365 nm.

60th Indian Pharmaceutical Congress PA-233 (2008). HPTLC of glycyrrhizic acid on silica gel with toluene - ethyl acetate - glacial acetic acid 25:15:1. The hRf value of glycyrrhizic acid was 52, linearity was between 500-1000 ng/mL and recovery was 99.2 %. Withanolide A was separated with toluene - ethyl acetate - glacial acetic acid 20:20.1. The hRf value of withanolide A was 44, linearity was in the range of 400-1000 ng/mL and recovery was 99.4 %.