J. Planar Chromatogr. 5, 124-130 (1992). HPTLC of thyreostatic drugs (thiouracil, methylthiouracil, tapazole, propylthiouracil, phenylthiouracil) on silica with dichloromethane - methanol 98:2 in the first direction; after drying and adding the thyreostatic drug assumed to be present development in the second direction with dichloromethane - propionic acid 98:2. Detection via fluorescence induction of the 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole derivatives of the drugs with cysteine; quantification by densitometry in the fluorescence mode.

J. Planar Chromatogr. 8, 388 - 392 (1995). HPTLC of MDA.HCl, MDMA.HCl, MDE.HCl, BDB.HCl, MBDB.HCl, LSD, and atropine on silica with dichloromethane - methanol 200:3 in the presence of NH3 in a separate 10 mL glass vessel for LSD, ethyl acetate - acetone - methanol - 25% NH3 50:50:20:1 for MBDB, acetone - ethanol - 25% NH3 90:7:3 for atropine. Identification by in situ FTIR measurement.

J. Planar Chromatogr. 11, 309-310 (1998). TLC of phenytoin on silica gel with hexane - acetone 6:4 in a previously saturated TLC chamber. After drying the plate was sprayed with 0.1% bromine solution in carbon tetrachloride, and after 5 min sprayed with 0.1% o-toluidine (in 0.5% acetic acid) reagent. A blue spot of the quinoid oxidation product of o-toluidine appeared at Rf 0.65. The reagent gives a similar color reaction with some benzodiazepines.

Analysis of choline esterase inhibitors. CBS 83, 4-5 (1999) HPTLC-AMD of choline esterase inhibitors on silica gel with a 15-step gradient from methanol via dichloromethane to hexane. Detection by spraying with butyryl choline esterase solution followed by incubation at 37 °C for 30 min, and spraying with fast blue salt mixed with alpha-naphthyl acetate followed by incubation at 37 °C for 5 min. Quantitative determination by absorbance measurement at 254 nm.

J. AOAC Int. 90, 1203-1209 (2007). HPTLC of terpenelactones (total bilobalide, gingkolide A, and gingkolide B) on prewashed and sodium acetate preimpregnated silica gel with toluene - ethyl acetate - acetone - methanol 50:25:25:3 or ethyl acetate - hexane 9:1; also HPTLC of flavonol glycosides (quercetin, kaempferol, isorhamnetin as standards) on prewashed and preimpregnated silica gel with chloroform - acetone - formic acid - acetic acid 50:11:6:6. Plates were developed in solvent equilibrated, vapor saturated twin-trough chambers at 30°C. Densitometry in absorbance mode at 370 nm (for aglycones) and at 290 nm following a 1 s immersion in acetic anhydride and heating at different temperatures for varying lengths of time (for terpenelactones). Good relationship (95%) was determined between HPTLC and HPLC for determination of total flavonol glycosides. The HPTLC flavonol aglycone method also performed well in terms of accuracy and consecutive plate repeatability.

Indian J. Pharm. Sci. 69(3), 473 (2007). Dried leaves were Soxhlet extracted with methanol and concentrated. HPTLC of andrographolide in Andrographis paniculata on silica gel with chloroform - methanol 7:1 with chamber saturation for 15 min. Densitometric evaluation at 232 nm. The hRf value of andrographolide was 35, of neoandrographolide 15 and of andrographoside 3. The linearity range was 200 - 1000 ng/zone. Average andrographolide contents were 1.56 % in dried leaves sample.

J. Planar Chromatogr. 20, 73-74 (2007). HPTLC of hedychenone (a trimethyldecalin terpene) on silica gel with n-hexane - ethyl acetate 4:1 in a saturated twin-trough chamber. After development the plates were dried at 105 °C for 20 min. Densitometric evaluation in absorbance mode at 254 nm.

Phytochem. Anal. 19, 244-250 (2007). HPTLC of taraxerol in Clitoria ternatea on silica gel with hexane - ethyl acetate 4:1. Detection by spraying with anisaldehyde reagent. Quantitative determination by absorbance measurement at 420 nm. The hRf value of taraxerol was 53 and selectivity regarding the matrix was given. Linearity was between 100 and 1200 ng of taraxerol. Recovery values were between 99.6 and 99.7 %. The limit of detection and quantification for taraxerol was 31 and 105 ng/spot, respectively.