J. Liq. Chrom. Rel. Technol. 27, 1377-1411 (2004). The review includes 117 recent references regarding modern and efficient TLC methods like: gradient elution (GE) TLC, automated multiple development (AMD), and over-pressured layer chromatography (OPLC). Detailed discussions are included about the mobile phase optimization by the “Prisma” method and other mathematical models. The analysis and quantification by photodensitometry of flavonoids, coumarins, saponins, alkaloids, and other classes of compounds include detailed experimental conditions, stationary and mobile phase, development methods and quantitative determination by densitometric procedures.

J. Planar Chromatogr. 18, 290-293 (2005). TLC of gallic acid in tea extracts (gallic acid, caffeine, (+)-catechin, and tannic acid as standards) on silica gel in an unsaturated chamber with chloroform - ethyl acetate - formic acid 5:4:1. Densitometric measurement at 289 nm. Limit of detection 0.1 µg per spot.

Planta Med. 71, 764-769 (2005). Preparative TLC of 9 known diterpenoids and shikokianin and rabdoternin A on silica gel by two-fold development with chloroform - methanol 30:1, and of rabdosichuanin and lasiokaurin with chloroform - acetone 6:1. Detection under UV light at 254 nm.

SR 82. 2) Dünnschichtchromatographische Identifizierung der Wirkstoffe in Tabulettae antidolorosae fortes SR 82. (1. Thin-layer chromatographic detection of the active substances in oculoguttae. 2. Thin-layer chromatographic identification of the active substances in tabulattae...) Pharmazia 37, 603 (1982). TLC of 1) prednisolone, chloramphenicol on silica with ether and of 2) papaverine, aminophenazone, caffeine and phenacetin on silica with ethanol - water 10:40. Detection by UV.

Planta Med. 70, 1095-1097 (2004). Preparative TLC of (+/-)-trans-3-(4-hydroxy-3-methoxyphenyl)-4-[(E)--3,4-dimethoxystyryl]cyclohex-1-ene and related compounds on silica gel with n-hexane - ethyl acetate 2:1 and n-hexane - acetone 3:2. Detection under UV light at 254 nm.

J. Chromatogr. Biomed. Applic. 273, 19-42 (1983). The chemical structure of a compound influences not only the analytical method best suited to its quantification, but also its acid / base character (pKa) and its extractability. The dose administered, the bioavailability of the dosage form, and the pharmacokinetic profile of the drug govern the circulating concentrations of either the parent drug and / or its metabolites present in vivo, and dictate the ultimate sensitivity and specificity required of the analytical method. The degree of sample preparation required is dependent on the analytical method used (gas-liquid chromatography, TLC, HPLC) and on the tolerance of the specific type of detection system to contamination. Factors leading to compound losses during sample preparation (adsorption, stability) are critical at low concentrations and can adversely affect the reliability of an assay, therefore maximizing the overall recovery of the assay is essential not only for high sensitivity but also for good precision and accuracy.

by automated multiple development. J. Planar Chromatogr. 19, 115-117 (2006). HPTLC of eight ginsenosides on silica gel after cleaning with isopropanol for 1 h with methanol - chloroform. Two gradient programs and two different values of increment in the development distance were compared. Visualization by spraying with A) Godin’s reagent (5% solution of sulfuric acid in ethanol), and B) 1% solution of vanillin in ethanol, followed by heating at 105°C for 10 min. Evaluation by scanning at 540 nm.

J.A.O.A.C. 66, 884-892 (1983). TLC of sulfamethazine, sulfadimethoxine, sulfaquinoxaline, sulfathiazole, sulfabromomethazine, sulfapyridine on silica with 1a) methanol for 1 cm, 1b) chloroform - tert. butanol 8:2 for 6 cm, 1c) chloroform - tert. butanol 8:2 for 6 cm. Between each development drying of the plate for 1 minute at 110 °C. Derivatization of compounds by dipping into fluorescamine solution. Fluorometry.