Indian Drugs 38 (10), 542-542 (2001). TLC hydrolyzed extract mixture on silica gel (impregnated with boric acid and activated) with acetic acid - chloroform - water 8:5:1. Detection by spraying with aniline phthalate reagent. TLC of two selectively hydrolyzed fractions on silica gel with acetonitrile - water 17:3. Detection by spraying with sulfuric acid - water 1:3 and heating. Presence of b-galactose and fructose was confirmed.

(Chinese) J. Chinese Trad. and Herb. drugs 36 (1), 132-137 (2005). A review with 22 references on TLC-bioautography, including the screening of natural compounds with antibacterial and/or antifungal activity, cholinesterase inhibitors, free radical eliminators, and antioxidants. Discussion of the advantages of the technique compared to other related techniques.

J. Liq. Chromatogr. Relat. Technol. 36, 167-179 (2013). HPTLC of darunavir in rat plasma on silica gel with toluene - acetone - methanol 3:1:1. Quantitative determination by mass spectrometry in the ESI+ mode and absorbance measurement at 254 nm. The hRf value of darunavir was 53. Linearity was in the range of 5-150 ng/µL. LOD and LOQ were found to be 1.2 and 3.9 ng/µL, respectively. Intermediate precision was below 2.7 %. Recovery (by standard addition) was 94.8-98.4 %. The recovery of the drug is improved compared with the HPLC method (96.7 %).

J. Planar Chromatogr. 20, 457-461 (2007). HPTLC of rivastigmine ((-)-S-N-ethyl-3-[(1-dimethylamino)ethyl]-N-methylphenylcarbamate) and impurity on silica gel with chloroform - methanol 2:3 in a twin-trough chamber with chamber saturation for 30 min. Densitometric analysis was performed in absorbance mode at 210 nm. Limits of detection were 30 and 100 ng/zone, respectively.

Proc. Intern. Symposium on TLC with special Emphasis on OPLC, Szeged, 15 (1984). OPLC (OPTLC) of atropine, scopolamine, scopoline, tropine, apoatropine on silica with a) isoamyl alcohol - propanol - chloroform- 0.1 M CH3COONa in water 7:23:5:5, b) butanol - methanol - chloroform - 0.1 M CH3COONa in water 8:12:5:5. Detection with Dragendorff reagent. Densitometry by absorbance at 400 nm.

CBS 98, 2-4 (2007). HPTLC of amino-3-propan-1-ol, a degradation product of dexpanthenol, on silica gel with ethanol - water - acetic acid 16:3:1. To the mobile phase the derivatization reagent was added, i.e. 0.5 g ninhydrin were dissolved in 100 mL solvent mixture. Development in the horizontal developing chamber from both plate sides over 40 mm. Detection by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 486 nm. The hRf value of amino-propanol was 50. The mean repeatability was 4.9 % at 5 different concentration levels. The relative standard deviation of the intermediate precision (n=9) was 5.7 %. The limit of detection and quantification was 4.5 µg/mL and 15 µg/mL, respectively (related to the application volume of 2 µL). Recovery (n=15) was 102 %.

J. Liquid Chrom. 10, 1291-1298 (1987). TLC of amniotic fluid and phospholipid standards on silica with chloroform - ethanol - triethylamine - water 30:34:30:8. Detection: after drying at 170 °C for 2 min. spraying with 10 % solution of copper sulfate in 8 % H3PO4, and 5 % copper acetate in 8 % H3PO4 and heating again at 110 °C for 5 min.; after inspection, heating up to 170 °-180 °C.

J. Planar Chromatogr. 20, 145-147 (2007). HPTLC of clotrimazole and tinidazole on silica gel in a twin-trough chamber with toluene - ethyl acetate - methanol - glacial acetic acid 60:30:10:3 with chamber saturation for 10 min. Quantitation by scanning at 254 nm.