Microbiol. Res. 268, 127295 (2023). HPTLC of cardiolipin phospholipids in the deletion mutants of two cardiolipin synthetase genes, clsA (UM270 ΔclsA) and clsB (UM270 ΔclsB), in the rhizobacterium Pseudomonas fluorescens, on silica gel with chloroform - methanol - water 14:6:1 for the first dimension and chloroform - methanol - glacial acetic acid 13:5:2 for the second dimension. The lipid composition of UM270 wt, UM270 ΔclsA and UM270 ΔclsB mutant strains was determined by labeling with [1-¹⁴C] acetate. Detection by iodine staining and radioactive membrane lipids were visualized by exposure to autoradiography film or a Phosphor Imager screen. Individual lipids were quantified using an image software.

J. Agric. Food Chem. 56, 8050-8057 (2008). HPTLC of [2-14C]-cymoxanil in the cultures (medium and mycelium) and in the cell-free extracts of Botrytis cinerea on silica gel with hexane - ethyl acetate - acetic acid 70:30:1. The most polar products were separated on RP-18 (impregnated with a methanolic solution of tetrabutylammonium bromide 70 mM and dried at 80 °C for 5 min) with phosphate buffer (0.01 M, pH 6) – methanol 11:9. Detection by autoradiography. The hRf value of cymoxanil was 35 on silica gel. Different metabolites were detected using both stationary phases.

J. Chromatogr. A 1520, 9-22 (2017). A review focused on application of TLC as simple, rapid, popular and inexpensive method for lipophilicity assessment. Discussion of the principles and methodology of quantitative structure retention relationship (QSRR) employed for lipophilicity prediction from retention data. Comparison of traditional and unconventional TLC systems applied for lipophilicity assessment. Overview of the applications of TLC retention constants and use of bioautography as a TLC method complementary in quantitative structure activity relationship (QSAR) studies. Discussion of the advantages and limitations of the method, and application of less common planar chromatography modes for drug discovery process.

J. Agric. Food Chem. 39, 412-417 (1991). Analytical and preparative TLC on silica with 7 solvent systems (e.g. dichloromethane - ether 1:1), visualization by autoradiography or by UV; quantification after scraping by liquid scintillation. Also two-dimensional TLC.

(Chinese) J. Chinese Trad. and Herb. drugs 36 (1), 132-137 (2005). A review with 22 references on TLC-bioautography, including the screening of natural compounds with antibacterial and/or antifungal activity, cholinesterase inhibitors, free radical eliminators, and antioxidants. Discussion of the advantages of the technique compared to other related techniques.

J. Chromatogr. Sci. 48 (1), 26-28 (2010). HPTLC of cefuroxime axetil and ornidazole in combined tablet dosage form on silica gel with toluene - n-butanol - triethylamine 17:4:1. The hRf value of ornidazole was 51 and of cefuroxime axetil 67. Quantification by densitometry at 285 nm. Linearity was between 100 and 500 ng/band for both cefuroxime axetil and ornidazole. The method was used for the assay of the compounds in pharmaceutical formulations. The content of cefuroxime axetil was 102.4 % and of ornidazole 101.0 %.

Rapid conversion of the herbicide to ten thytoxic metabolites in a resistant plant, sugarcane. J. Agric. Food Chem. 33, 1070-1075 (1985). TLC and HPTLC separation of isouran and metabolites on silica or RP-8 plates with ether - acetone - chloroform - methanol 9:1, chloroform - methanol - pyridine - acetic acid 15:3:1:1 and methanol - water 7:3. Detection by autoradiography. Comparison with standards.

Arzneim.-Forsch./Drug Res. 41, 392-398 (1991). TLC of nimodipine ((±)3-isopropyl 5-(2-methoxyethyl)1,4-dihydro-2,6-dimethyl-4-(3-nitrophenyl)-pyridine-3,5-dicarboxylate) and metabolites on silica with chloroform - methanol - water - acetic acid 65:28:4:4; 2D TLC using toluene - acetone - acetic acid 70:25:5 and toluene - acetone 1:1. Detection by autoradiography and scintillation.