Abstract No. F-251, 61st IPC (2009). HPTLC of doxazosin mesylate on silica gel with acetone - toluene - 25 % ammonia 60:40:1. The hRf value was 65. Quantitative determination by absorbance measurement at 251 nm. Linearity was in the range of 20-100 ng/band. Recovery was 103.3 %.

60th Indian Pharmaceutical Congress PA-232 (2008). HPTLC of olmesartan medoxomil and hydrochlorothiazide on silica gel with methanol - toluene - ethyl acetate 5:11:4 with chamber saturation for 30 min. The hRf value of olmesartan medoxomil was 27 and of hydrochlorothiazide 44. The method was linear in the range of 300-1800 ng/spot for both drugs.

Abstract No. F-264, 61st IPC (2009). HPTLC of pantoprazole and itopride hydrochloride on silica gel with n-butanol - chloroform - 25 % ammonia 7:2:1. The hRf value was 54 and 75 for pantoprazole and itopride hydrochloride, respectively. Quantitative determination by absorbance measurement at 291 nm. The linearity of the method was 80-240 ng/band for pantoprazole and 300-900 ng/band for itopride.

IPA Convention, 2010, RA-PO 35. HPTLC of citicoline sodium in tablet formulation on silica gel with chloroform - methanol - water 3:7:3. The compound was well resolved with an hRf value of 53. Densitometric measurement at 280 nm. The method was linear in the range of 300-900 ng/band. HPLC analysis was performed on RP18 column using 1 % formic acid - methanol 19:1. Results obtained with either method were comparable.

J. Liq. Chromatogr. Relat. Technol. 33, 423-430 (2010). HPTLC of olmesartan and zidovidine on silica gel with ethyl acetate - methanol - acetic acid 160:40:1 in a twin-trough chamber saturated for 10 min. Quantitative determination by densitometric scanning at 269 nm. The linearity range was 80-600 ng/zone. LOQ was 80 ng/zone, the correlation coefficient 0.9900 and 0.9820. Recovery was 90.1 and 79.6 %. The accuracy and precision of the method were determined by repeatability (intra-day) and intermediate precision (inter-day) for the set of quality control samples (low, mid, high) in replicate. The results revealed excellent intra- and inter-day accuracy and precision of the method, which was within the acceptable limit (accuracy (% RE) 11.89 and 6.76 (low), 2.53 and 3.83 (mid), and 0.65 and 7.14 (high); inter-day precision (CV): 3.29 and 3.00 (low), 1.02 and 1.51 (mid), and 1.11 and 0.69 (high); intra-day precision: 2.59 and 2.86 (low), 1.07 and 1.13 (mid), and 1.04 and 0.72 (high) - after LLE and SPE, respectively).

J. AOAC Int. 93, 1222-1227 (2010). HPTLC of rosuvastatin (ROS) and ezetimibe (EZE) on silica gel with n-butyl-acetate - chloroform - glacial acetic acid 1:8:1 with chamber saturation for 30 min. Quantitative determination by densitometry at 245 nm. The hRf value of ROS was 30 and of EZE 58. The linearity for ROS and EZE was 0.1 to 0.9 µg/zone.The intraday and interday precision was 0.62 % and 0.73 % for ROS and 0.59 % and 0.69 % for EZE (n=3). The LOD values for ROS and EZE were found to be 0.04 and 0.07 µg/spot, respectively, and their LOQ values were 0.07 and 0.1 µg/spot, respectively. The recovery study results ranged from 98 to 101 % for ROS and EZE.

J. Liq. Chromatogr. Relat. Technol. 34, 2548-2464 (2011). HPTLC of tocopherol acetate (1) and tocopherol (2) in oral fluid vitamin E on silica gel with chloroform - cyclohexane 11:9. Quantitative determination by absorbance measurement at 202 nm for (1) and 272 nm for (2). The hRf values of (1) and (2) were 47 and 38, respectively. Linearity was in the range of 2-8 µg/band for (1) and (2). Limits of detection and quantification were found to be 50 and 150 ng/zone for (1). Precision was below 2 %. The intermediate/inter-day/intra-day precision was 0.4 % (n = 6). Recovery (by standard addition) was in the range between 99.8-101.5 %. Tocopherol acetate was better separated from tocopherol using normal phase TLC than by reversed phase TLC.

J. AOAC Int. 93, 904-910 (2010). HPTLC of imipramine hydrochloride and chlordiazepoxide on silica gel with carbon tetrachloride - acetone - triethylamine (pH 8.3) 20:10:1 in a twin-trough chamber after saturation for 30 min at 25 °C. Quantitative determination by densitometry in absorbance mode at 240 nm. Linearity was between 50-600 and 20-240 ng/zone with mean accuracies of 99.5 and 100.6 % for imiprazine hydrochloride and chlordiazepoxide, respectively. The hRf value of imipramine was 73 and of chlordiazepoxide 32. The %RSD values of intra-day and inter-day precision were between 0.7-1.4 % and 0.4-1.2 % for imipramine and 0.8-1.7 % and 0.7-1.3 % for chlordiazepoxide.