J. Planar Chromatogr. 10, 208-216 (1997). TLC of ionic associates of BZ, phencyclidine, LSD, morphine, codeine, ethylmorphine, scopolamine, physostigmine, cocaine, ephedrine on silica and alumina with 68 alkaline mobile phases. Ionic associates of the basic organics were formed with bromoxylenol blue, cresol red, and eriochromecyanine-R.

J. Planar Chromatogr. 13, 432-436 (2000). HPTLC of benzodiazepines (diazepam, prazepam, lorazepam, nitrazepam) on silica gel with chloroform - acetone 4:19. Visualization under UV 254 and 366 nm. Subsequent tandem mass spectroscopy for the analysis and identification of several common benzodiazepines; FAB-MS and MS-MS, directly from the silica matrix, without prior extraction, were successfully used both for standards and for urine extracts.

59th Indian Pharmaceutical congress C-305, 297, (2007). Phytoconstituents of mature and immature tubers of Ipomoea mauritiana (methanolic and aqueous extracts) have been studied. HPTLC on silica gel with chloroform - methanol - formic acid 6:3:1. Detection by spraying with vanilin - sulphuric acid reagent. Densitometric evaluation at 365 nm. Mature tubers were found to contain higher concentration of phytoconstiuents than immature tubers.

J. Sep. Sci. 30, 2086-2091 (2007). HPTLC of 4alpha-methyl-24beta-ethyl-5alpha-cholesta-14,25-dien-3beta-ol (1), 24beta-ethylcolesta-5,9,11,22E-trien-3beta-ol (2), and betulinic acid (3) in Clerodendrum inerme on silica gel with toluene - ethyl acetate 47:3. Quantitative determination by absorbance measurement at 620 nm. The hRf value was 48, 34 and 22 for compounds (1) , (2) and (3), respectively. Linearity was between 100 and 2500 ng/zone. The limits of detection and quantification were 5, 6, and 10 µg/mL and 14, 18, and 29 µg/mL, respectively, for (1), (2), and (3).

Chromatographia 66 (9-10), 801-804 (2007). HPTLC of withaferin A and withanolide A in Withania somnifera methanolic extract from different plant parts (leaf, root, stem and fruit) and of two morphotypes, on silica gel with toluene - ethyl acetate - formic acid 5:5:1. Quantification by densitometry in absorption mode at 530 nm. Linearity was between 200 and 3200 ng for both withaferin A and withanolide A. The average recovery of withaferin A and withanolide A was 96.0 and 96.7 %.

J. Food Comp. Anal. 21, 496-500 (2008). HPTLC of bergenin (1), catechin (2), and gallic acid (3) from Bergenia ciliata and Bergenia ligulata on silica gel with toluene - ethyl acetate - formic acid 4:6:1. Quantitative determination by absorbance measurement at 280 nm. The hRf values of (1), (2), and (3) were 29, 54, and 60, respectively. Selectivity regarding matrix was given. Linearity was between 160 and 800 ng/spot for (1), 160 and 480 ng/spot for (2), and 160 and 560 ng/spot for (3). The limits of detection and quantification were 120 and 160 ng for (1), 80 and 120 ng for (2), and 40 and 80 ng for (3), respectively. Recovery was 99.3 % for (1), 98.6 % for (2), and 99.2 % for (3). The intermediate/interday/intra-day precision (n=6) was 0.04 % and 0.07 % for (1), 0.07 % and 0.06 % for (2), and 0.02 % and 0.11 % for (3).

J. AOAC Int. 91, 13-20 (2008). Comprehensive proposal for the validation of qualitative HPTLC methods. The steps of the validation process (method selection and optimization, stability, specificity, precision, and robustness) are illustrated with examples of identification methods: green tea leaf, ginseng root, eleuthero root, echinacea root, black cohosh rhizome, licorice root, kava root, milk thistle aerial parts, feverfew aerial parts, and ginger root. The validation protocol is a key element for structuring, managing and documenting the validation process. HPTLC is suitable for reliable identification of botanicals because it can provide chromatographic fingerprints that can be visualized and stored as electronic images. Reproducibility is improved if suitable instrumentation is used, a standardized HPTLC methodology is implemented, and methods have been validated.

& Perry. J. AOAC Int. 91, 1169-1173 (2008). HPTLC of gallic acid, caffeic acid, and syringic acid (in clove) on silica gel with toluene - ethyl acetate - formic acid 8:2:1 in a twin-trough chamber. Quantitative determination by absorbance measurement at 280 nm.