Chinese J. Trad. Patent Med. (Zhongchengyao) 26 (8), Appendix 16-18 (2004). Study of the identification of Shenbao capsules by TLC, in addition to microscopy and chemical methods. TLC of Shenbao capsule extracts on silica gel with 1) the lower phase of chloroform - ethyl acetate - methanol - water 15:40:22:10; 2) toluene - methanol 4:1. Detection 1) by spraying with 10 % H2SO4 in ethanol followed by heating at 105 ºC; 2) under UV light. Identification by fingerprint technique. Combination of the procedure with microscopy and chemical methods allows identification of all drug ingredients.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27(2), 150-154 (2005). TLC on silica gel with 1) chloroform - methanol 15:2; 2) n-hexane - ethyl acetate 9:1; 3) toluene - ethyl acetate - acetone - methanol 50:25:25:3. Detection 1) by spraying with 10 % phosphomolybdic acid and heating; 2) under UV 365 nm; 3) by exposing to acetic anhydride vapours and heating at 140 - 160 ºC and under UV 365 nm. Identification by fingerprint technique. Quantification of flavone glycoside by HPLC. The results for three real life samples are given.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27(5), 535-538 (2005). TLC of the extracts on silica gel with 1) benzene - ethyl acetate - formic acid 15:2:1; 2) n-hexane - ethyl acetate - formic acid 60:20:1; 3) chloroform - methanol - ammonia 40:10:1; 4) chloroform - methanol - water 13:7:2. Detection 1) under UV 365 nm; 2) by spraying with 3 % ninhydrin solution followed by heating at 105 ºC until the spots are visualized; 3) by spraying with 10 % H2SO4 solution in ethanol followed by heating until the spots are visualized. Identification by fingerprint technique. Quantification of emodin by densitometry at 445 nm. Validation of the method by investigation of its linearity range (0.1 µg - 1.0 µg, r = 0.998); precision (RSD = 1.05 % n = 6); reproducibility of six time assay towards the same sample (RSD = 1.24 %); and standard addition recovery (96.7 %, RSD = 1.75 %, n = 6). The results for three batches of real life sample are given.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27(7), 775-777 (2005). TLC of extracts of Suzi Jiangqi pills on silica gel with 1) petroleum ether (60 - 90 ºC) - ethyl acetate 9:1; and 2) chloroform - ethyl acetate - methanol - water 15:40:22:10. Detection 1) under UV 365 nm; 2) 10 % H2SO4 in ethanol followed by heating until the spots are visualized. Identification by fingerprint techniques. Quantification of hespiridin by HPLC. The results for five batches of real life samples are given. Discussion of the application of the procedures for the quality control of the medicine.

Whole Plant Powder by HPTLC. Chromatographia 65 (11-12), 767-769 (2006). Description of a simple, precise and accurate HPTLC method for the determination of rutin in the whole plant powder of Amaranthus spinosus Linn, which has been reported to have anti-diabetic, anti-thrombotic, anti-inflammatory and anti-carcinogenic activity. TLC of a methanol extract of the whole plant powder on silica gel with ethyl acetate - formic acid - methanol - water 100:9:11:17. Quantitative determination by densitometric measurement in absorbance mode at 363 nm. Linearity was between 10 and 60 µg/mL.

J. Planar Chromatogr. 22, 29-33 (2009). HPTLC of theophylline (extracted at pH 8.5 with chloroform - isopropanol 4:1 from post-mortem blood after acid hydrolysis) on silica gel in a twin trough chamber with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 277 nm. Polynomial regression in the range of 0.5-20 µg/mL. The limit of detection was 0.5 µg/mL (S/N = 3). Intra-day and inter-day repeatability was between 0.5-0.8 % and 0.5-1.3 %, respectively, for three different concentrations in the range of 0.5-10 µg/mL. Recovery was 89.1-93.4 % at a concentration of 10 µg/mL and pH 8.3-8.6. An average analytical recovery of 89.9 % was achieved at pH 8.5 with a relative standard deviation of 2.2 %. Theophylline was stable in methanolic solution and during chromatography.

Budapest Chromatography Symposium, June 13, 1985. EGYT 1855: TLC on silica with chloroform in the first dimension, ethyl acetate - propanol -10 % acetic acid 2:2:1 in the second dimension. Staining with Dragendorff reagent; densitometry by absorbance at 450 nm. GYKI 51 189: TLC on silica with benzene in the first dimension, benzene - ethyl acetate 20:8 in the second dimension; detection by UV 254 nm, densitometry by absorbance at 300 nm.

Anal. Chim. Acta 225, 137-146 (1989). One- and two-dimensional TLC of residues of veterinary tranquillizers and the ß-blocker carazolol on silica with 1) acetone – dichloromethane – 25% NH3 40:60:1 and 2) acetone – 25% NH3 100:0.1 and 3) acetone. Detection by spraying with 1) Dragendorff’s reagent, 2) vanillin-phosphoric acid and 3) sulfuric acid – ethanol – ether 2:10:2. Limits of identification range, 1–2.5 µg/kg.