J. Planar Chromatogr. 25, 433-438 (2012). HPTLC of quercetin (1) and kaempferol (2) in the leaves of Centella asiatica on silica gel with toluene - ethyl acetate - chloroform - formic acid 6:4:4:1. Quantitative determination by absorbance measurement at 240 nm. The hRf of compounds (1) and (2) were 35 and 48, respectively. Linearity was in the range of 100-1000 ng/band. Limits of detection and quantification were 54 and 165 ng/band for (1) and 68 and 207 ng/band for (2), respectively. Intra-day relative standard deviation of (1) was between 5.3 and 6.5 % and of (2) between 5.1 and 11.4 %. Inter-day relative standard deviation of (1) was 2.1-6.6 % and of (2) 2.6-5.8 % (n=6). Recovery was found to be 98.1 % for (1) and 90.1 % for (2).

J. of China Pharm. 19 (22), 41-43 (2010). Yanreqing capsules are a herbal TCM preparation specially effective for heat clearing and detoxifying, and prescribed clinically to cure bronchitis, pneumonia, acute tonsillitis, urinary system infections and biliary tract infections. TLC of the extracts of the medicine on silica gel 1) for Baical Skullcap, with ethyl acetate – butanone – formic acid – water 5:3:1:1, detection by spraying with 2 % ferric chloride in ethanol and viewing in daylight; 2) for Cape jasmine, with ethyl acetate – acetone – formic acid – water 5:5:1:1, detection by spraying with 10 % sulfuric acid in ethanol and heating at 110 °C until the zones are visible.

Brazilian Journal of Pharmacognosy 22, 1355-1361 (2012). HPTLC of piperine (1) and piperlongumine (2) on silica gel with toluene - ethyl acetate 3:2 as mobile phase. Quantitative determination by absorbance measurement at 342 and 325 nm for (1) and (2), respectively. The hRf of compounds (1) and (2) were 51 and 74, respectively. Linearity was in the range of 20-100 ng/zone for (1) and 30-150 ng/zone for (2). Limits of detection and quantification were 7 and 20 ng/zone for (1) and 10 and 30 ng/zone for (2), respectively. Intermediate/intra-day/inter-day precision was below 1.1 % (n=6). Recovery for both (1) and (2) was between 93.7 and 96.7 %.

Chinese J. of Trad. Chinese Med. for Emerg. 21 (5), 738-739 (2012). Ningxin pills are a TCM preparation with special efficiency on promoting blood circulation, removing blood stasis, relieving pain and are prescribed clinically to treat coronary disease and angina pectoris. For quality control, TLC of the extracts on silica gel 1) for Panax Notoginseng (Burk.) F.H.Chen, Radix Ginseng rubra, with the lower phase of chloroform – ethyl acetate – methanol – water 15:40:22:10, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 2) for Rhizoma Corydalis, with toluene – acetone 9:2, detection by exposure to iodine vapors and evaluation at UV 366 nm; 3) for borneol, with petroleum ether (30-60 ºC) – ethyl acetate – chloroform 11:1:3, detection by spraying with 1 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing in daylight.

Chinese J. of Inform. on TCM 17 (12), 45-48 (2010). Jinshishenyan pills are a herbal TCM preparation effective for nourishing kidney, activating spleen-energy, relieving internal heat or fever, and removing blood stasis. For quality control, TLC of the extracts on silica gel for 1) Lonicera japonica, with the upper phase of ethyl acetate – formic acid – water 14:5:5, detection at UV 366 nm; 2) for Cortex Phellodendri chinensis, developed with benzene - ethyl acetate – methanol – isopropanol – concentrated ammonia 12:6:3:3:1, detection by exposure to ammonia vapors for a few minutes and evaluation at UV 366 nm.

Chinese J. of Today’s Pharm. 22 (11), 690-692 (2012). Qufengjiangu tablets are a herbal TCM for the treatment of waist and knee pain and rheumatic arthralgia. For quality control, TLC on silica gel 1) for Radix Angelicae Pubescentis, with cyclohexane – ethyl acetate – formic acid – glacial acetic acid 70:20:2:3, detection under UV 366 nm; 2) for Radix Notoginseng and Radix Dipsaci, with n-butanol – ethyl acetate – 10 % diluted ammonia 4:1:5, detection by spraying with 10 % sulfuric acid in ethanol and heating at 105 °C, viewing in daylight; 3) Herba Taxilli, with toluene saturated with water – ethyl formate – formic acid 5:4:1, detection by spraying with 5 % aluminium chloride in ethanol and heating at 105 °C, viewing under UV 366 nm. Quantification of osthole by HPLC.

J. of China Pharm. 26 (4), 368-369 (2012). Nauclea officinalis is a tree growing in tropical regions. In TCM its branch, trunk and bark are used for treatment of acute tonsillitis, acute pharyngitis, conjunctivitis, upper respiratory tract infection, etc. For quality control of its decoction pieces and the three formulations, Danmu injection, Danmu Jingao tablets, and Danmu Jingao syrup, TLC on silica gel with n-hexane – ethyl acetate – methanol 15:8:2, detection under UV 366 nm. As standards the alkaloids naucleficine, mauclefidine, and nauclefline were used.

J. Liq. Chromatogr. Relat. Technol. 36, 197-212 (2013). HPTLC of lupeol (1) and stigmasterol (2) in the stems of Costus igneus on silica gel with n-hexane - ethyl acetate 4:1 for (1) and toluene - acetone - acetic acid 89:9:2 for (2). Detection by dipping in a solution of 0.5 mL p-anisaldehyde in 50 mL glacial acetic acid and 1 mL of 97 % sulfuric acid, followed by heating at 105 °C. Quantitative determination by absorbance measurement at 538 nm. The hRf values of (1) and (2) were 55 and 58, respectively. Linearity was in the range of 5-10 µg/zone for (1) and 1-6 µg/zone for (2). LOD and LOQ were 131 and 430 ng/zone for (1) and 80 and 212 ng/zone for (2). Intermediate precision was below 2.9 %. Average recovery (by standard addition) for (1) and (2) was 100.2 % and 99.9 %, respectively.