Shimadzu Hyoron, (Shimadzu Review) 45, 199-202 (1988). TLC on silica with different solvent systems. Detection by spraying with different reagents or under UV. Quantification by densitometry. Detection limit for glycyrrhizin, geniposide, capsaicine, kainic acid, atropine and scopolamine, 0.01-0.1 µg/spot.

Arzneim.-Forschung / Drug Res. 44, 469-471 (1994). HPTLC of flunitrazepam, trimipramine, carbamazepine, diclofenac on silica with 3 different solvent systems: 1. chloroform - acetone 85:15, 2. ethyl acetate - ethanol - NH3 100:10:3, 3. dichloromethane - acetone 12:1; examination under UV 366 nm; quantification by fluorodensitometry; recording of UV-spectra between 200 and 400 nm (absorbance).

J. Planar Chromatogr. 9, 280-281 (1996). HPTLC of heroin, opium, mandrax tabl., diazepam, oxazepam, nitrazepam, phenobarbitone, and caffeine on silica with chloroform - ethanol 9:1. Detection after drying for 5 min at 100°C by spraying with a 1:1 mixture of 1% aqueous solutions of cupric chloride and potassium ferricyanide resulting in a dark brown spot for heroin. The reagent does not give positive reaction to the usually occurring adulterants as barbiturates, benzodiazepines, methaqualone, and caffeine, etc. Sensitivity: about 1 mg of heroin/spot.

J. Liq. Chrom. & Rel. Technol. 22, 161-171 (1999). OPLC of 82 basic drugs and metabolites on HPTLC silica gel with trichloroethylene - methyl ethyl ketone - n-butanol - acetic acid - water 17:8:25:6:4 and butyl acetate - ethanol - tripropylamine - water 340:37:20:3. Densitometry. The system can be used in the screening for drugs in autopsy urine samples, utilizing automated identification by hRf/UV library search.

Leg. Med. 8, 184-187 (2006). HPTLC on silica gel of cocaine urine samples submitted to solid phase extraction prior to derivatization (methylation) with diazomethane. For methylation samples were mixed with 100 µL of a solution freshly prepared by distillation of 2.14 g N-methyl-N-nitroso-p-toluenesulfonamide with 10 mL potassium hydroxide 96 % in ethanol and 30 mL ethyl ether, and kept at room temperature for 1 min to convert benzoylecgonine to cocaine. Development over 7 cm in a saturated chamber with ethyl acetate – cyclo hexane – ammonia 250:100:1. Detection by spraying with Dragendorff reagent (10 mL of 40 % m/v potassium iodide in water; 10 mL of 1 N solution of bismuth nitrate in glacial acetic acid; 80 mL of 10 % v/v sulfuric acid water solution; 2 g of resublimed iodine). The technique is capable to discriminate cocaine from interfering substances such as nicotine, caffeine and even cocaethylene in urine samples. The limit of detection was 100 ng of cocaine.

59th Indian Pharmaceutical congress C-323, 302,(2007). TLC of flavonoids and sapogenins from different plant parts (leaves and petals) of Calendula officinialis on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:20, and chloroform - glacial acetic acid - methanol - water 15:8:3:2.

and Dietary Supplements. Chromatographia 66 (9-10), 797-800 (2007). HPTLC of 20-hydroxyecdysone from Sida rhombifolia L. on silica gel plates with chloroform - methanol 4:1. Quantification by densitometry at 250 nm in absorbance mode. Linearity was between 200 and 1000 ng/zone. This method was successfully applied for quantitative evaluation of dietary supplements. In addition, for six different Sida species unique fingerprints were obtained on the HPTLC plate.

CBS 97, 12-15 (2006). HPTLC of flavonoids from green tea (Camellia sinensis) on silica gel in a saturated twin-trough chamber with ethyl formate - toluene - formic acid - water 60:3:8:6. Detection by dipping the hot plate (heated at 100 °C for 2 min) into natural products reagent, followed by drying, dipping into polyethylene glycol 400 (10 g in 200 mL dichloromethane), and drying. Evaluation under UV 366 nm. With this method the geographical origin of the material can be determined. Toluene - acetone - formic acid 9:9:2 allows the discrimination of green from black and other speciality teas, based on the polyphenol pattern. Detection by dipping the hot plate into a solution of Fast Blue Salt B. Evaluation under white light. For investigation of the alkaloid profile ethyl acetate - methanol - water 20:2.7:2 and evaluation under UV 254 nm is used. The amino acid profile is analyzed by using 1-butanol - acetone - acetic acid - water 7:7:2:4. Detection by dipping in ninhydrin reagent, followed by heating at 110 °C for 3 min. Evaluation under white light.