59th Indian Pharmaceutical congress F-217, 442, (2007). HPTLC of chloroform, ethanol and water extracts of Nothapodytes foetida on silica gel with toluene - ethyl acetate - glacial acetic acid 25:5:1. Densitometry at 366 nm. The hRf value of camptothecin was 22. Linearity was between 0.5 and 5 ng/zone. All commercial samples of the plant had a similar fingerprint profile.

Food Chem. 108, 1039-1053 (2008). HPTLC of luteolin-7-O-beta-glucuronide (1), lithospermic acid (2), and rosmarinic acid (3) in Lamiaceous species (Thymi herba, Serpylli herba, and Majoranae herba) on amino phase for (1) and silica gel for (1), (2), and (3) with acetone - formic acid 17:3 and diisopropyl ether - acetone - formic acid - water 5:3:1:1, respectively. Evaluation at 365 nm before and after spraying with 2 % methanolic aluminium chloride, natural product/polyethylene glycol reagent or in visible light after treatment with bis-diazotized sulfanilamide (yellow, orange, red, and brown bands).

Pharmazie 63, 389-393 (2008). TLC of methyl brevifolincarboxylate and kaempferol 3-O-beta-D-(6’’-E-p-coumaroyl)-glucopyranoside tiliroside on silica gel with ethyl acetate - methanol - water 18:1:1. Detection under UV light.

Anal. Chim. Acta 577 (1), 46-51 (2006). HPTLC of trigonelline in herbal extracts and in pharmaceutical dosage forms on silica gel with n-propanol - methanol - water 4:1:4. Quantitative determination by absorbance measurement at 269 nm. Linearity was between 100 - 1200 ng/spot (via peak area) and the correlation coefficient was 0.9991. The trigonelline content of herbal extracts quantified and estimated from the formulation was found to be well within limits (± 5 %) of the labeled content of the formulations. The method is reproducible and selective for the estimation of trigonelline by statistical analysis of the data.

J. Planar Chromatogr. 21, 233-236 (2008). Connection of two-dimensional chromatography with multiple gradient development. 2D HPTLC of anthraquinone derivatives (1,8-dihydroxyanthraquinone, franguloemodin A, aloeemodin, rhein, frangulin A, aloin, sennoside B) on silica gel. Bandwise application at the right and left corner of the plate, then development by use of multiple gradient development [G. Matysik, Chromatographia 43, 39-41 (2004)], e.g. for step 1 hexane - dichloromethane 1:1, for step 2 (hexane - dichloromethane - ethyl acetate - 80 % formic acid 50:40:10:1) and for step 3 hexane - dichloromethane - ethyl acetate - methanol - formic acid 40:40:20:5:1. After drying the plate was developed from the left and right edge with hexane - dichloromethane - formic acid 60:40:1 for step 1 and with hexane - dichloromethane - ethyl acetate - formic acid 60:35:5:1 for step 2. Quantitative determination by absorbance measurement at 440 nm. Deteciton of anthranoids in daylight and after derivatization with 10 % potassium hydroxide in methanol.

CBS 102, 10-12 (2009). HPTLC of diterpene lactones (ginkgolides A, B, C, and bilobalide) in aqueous Ginkgo biloba leaf extracts on silica gel (impregnated by dipping into a 4 % solution of sodium acetate in methanol - water 3:2 for 5 s followed by drying at room temperature for 1 h) with toluene - acetone 7:3 with chamber saturation for 20 min. After twofold development over 60 mm the plate is heated at 150 °C for 1 h for detection of active compounds. Quantitative determination by absorbance measurement at 254 and 400 nm. The correlation coefficients of the calibration curves were = 0.9971 and relative standard deviations were = 2.0 %. Intraday precision was 1.1-1.2 %, interday precision 1.1-1.3 % and recovery 98.5-104.6 %. The average content in leaves was 0.078 (in % of dry weight) for ginkgolide A , 0.072 for ginkgolide B, 0.076 for ginkgolide C, 0.062 for bilobalide and 0.29 % for total diterpene lactones.

60th Indian Pharmaceutical Congress PG-251 (2008). HPTLC of glycyrrhetinic acid and piperine in haematinic herbomineral capsule formulation on silica gel with toluene - ethyl acetate - glacial acetic acid 25:15:1. Quantitative determination by absorbance measurement at UV 254 nm. The method was linear in the range of 0.8-2.4 ng/spot (glycyrrhetinic acid) and 10-50 ng/spot (piperine). Recovery was 96.3-98.6 %.

60th Indian Pharmaceutical Congress PA-224 (2008). HPTLC of aloe-emodin in extract of Rheum Emodi (prepared by supercritical fluid extraction) on silica gel with toluene - acetone - formic acid 80:20:1. Quantitative determination by absorbance measurement at 254 nm. Linearity was in the range of 100-400 ng/spot. Compared with other extraction techniques supercritical fluid extraction was more effcient and less time consuming.