J. AOAC Int. 92, 1082-1087 (2009). HPTLC of alprazolam and fluoxetine hydrochloride in pure powder and formulations on silica gel with acetone - toluene - ammonia 12:7:1 in a twin trough chamber saturated for 30 min. Quantitative determination by absorbance measurement at 230 nm. There was no significant difference in the determined content of alprazolam and fluoxetine by HPTLC and HPLC methods (assay results compared by applying the paired t-test).

Abstract No. F-275, 61st IPC (2009). HPTLC of ivabradine HCl on silica gel with methanol - chloroform 1:1. The hRf value was 59. Quantitative determination by absorbance measurement at 285 nm. Linearity was in the range of 100-800 ng/spot with r2=0.9989 (via peak area).

Abstract No. F-257, 61st IPC (2009). HPTLC of tadalafil on silica gel with n-hexane - ethyl acetate - acetonitrile 14:3:3. The hRf value was 65. Quantitative determination by absorbance measurement at 215 nm. The method was linear in the range of 10-60 ng/band. The drug was subjected to different stress conditions (acid, alkali, oxidative, photodegradation, thermal) and showed degradation under all stress conditions. Degradation products and excipients of the formulation were well separated from the main component.

J. Planar Chromatogr. 23, 75-78 (2010). HPTLC of fluphenazine hydrochloride on silica gel (prewashed with methanol) with methanol - water 9:1 in a saturated twin-trough chamber. Quantitative determination by absorbance measurement at 306 nm. Linearity was in the range of 100 to 500 ng/µL with a correlation coefficient of 0.998. LOD and LOQ were 1.45 and 4.40 ng/zone, respectively. Intra-assay and inter-assay precision, expressed as relative standard deviation (RSD), were in the range 0.73-1.77 % (n = 3) and 1.18-1.86 % (n = 9), respectively. Recovery of fluphenazine hydrochloride was between 98.3 and 101.5 %, with RSD not higher than 1.87 %. The method was selective for fluphenazine hydrochloride and the preservatives in the injections.

The Pharma Review 149-150 (2010). TLC on silica gel with chloroform - methanol - toluene 4:3. The hRf value was 16 and 66 for potassium clavulanate and cefpodoxime proxetil respectively. Densitometric analysis was carried out at 215 nm. The method was linear in the range of 500-2500 and 2000-10000 ng/band for cefpodoxime proxetil and potassium clavulanate respectively. The recoveries are in the range of 99.1-100.7 %.

J. Planar Chromatogr. 24, 441-444 (2011). HPTLC of plant extracts and 17 polyphenolic compounds (apigenin, apigenin-7-glucoside, ellagic acid, hyperoside, isoquercitrin, kaempferol, kaempferol-3-glucoside, kaempferol-3-glucuronide, luteolin, luteolin-7-glucoside, methyl brevifolincarboxylate, myricetin, quercetin, quercetin-3-glucuronide, rutin, tiliroside, ellagic acid 3,3’-di-O-methyl ether 4-xylopyranoside) on silica gel (prewashed with methanol) with toluene - ethyl formate - formic acid 7:5:1 in an automatic developing chamber set with a twin-trough chamber at 22 °C and a relative humidity of 48 %. Detection under UV light at 254 and 366 nm, and at 366 nm after spraying with 1.0 % methanolic diphenylborinic acid 2-aminoethylester.

J. Liq. Chromatogr. Relat. Technol. 34, 1502-1517 (2011). HPTLC of irinotecan in bulk drug and injectable formulations on silica gel with acetone - ethyl acetate 17:3 + 1 drop acetic acid. Quantitative determination by absorbance measurement at 366 nm. The hRf of irinotecan was 31. Linearity was 50-500 ng/zone. The LOD and LOQ was found to be 10 and 33 ng/zone. The intra-day precision was 4.3 % (n = 6) and inter-day precision over three different days was 3.1 %. Intra-day and inter-day accuracy were 96.9-100.3 % and 96.5-98.7 %, respectively. Recovery (by standard addition) ranged from 94.6-101.4 %.

J. Planar Chromatogr. 24, 367-372 (2011). TLC of piroxicam, meloxicam, tenoxicam, and isoxicam on silica gel with ethyl acetate - ethanol - toluene 6:3:1 + 2 drops of 25 % ammonia. Quantitative determination by densitometry in absorbance mode at 360 nm. Linearity was between 50-500 ng/zone. The hRf value was 53 for piroxicam, 78 for meloxicam, 61 for tenoxicam, and 82 for isoxicam. LOD were 10, 30, 10, and 20 ng/zone and LOQ were 20, 80, 40, and 40 ng/zone for piroxicam, meloxicam, tenoxicam, and isoxicam, respectively. The recovery was in the range of 93.2-102.9 % with %RSD of less than 1 %. The inter- and intra-day precision (%RSD) was between 0.3-0.8 %.