pruriens. Abstract No. 9425, IHCB (2009). HPTLC of L-dopa in Mucuna pruriens seed extract and formulations on silica gel with n-butanol - acetic acid - water 4:1:1. Quantitative determination by absorbance measurement at 280 nm. The method was linear in the range of 100-1200 ng/spot with an average recovery of 100.3 %.

Indian Drugs 46(6), 493-496 (2009). HPTLC of chloroform extracts of the bark of Ficus racemosa, F. bengalensis, and F. religiosa on silica gel with toluene - ethyl acetate - formic acid 90:10:1. Detection under UV 254 nm and visible light after treatment with vanillin sulfuric acid reagent, followed by heating at 105 °C until coloration.

60th Indian Pharmaceutical Congress PG-349 (2008). HPTLC of 3H-4M-benzaldehyde (in crude plant material, extracts and dosage form of Hemidesmus indicus) on silica gel with toluene - ethyl acetate - methanol - acetic acid 15:3:1:1. Quantitative determination by absorbance measurement at 230 nm.

J. AOAC Int. 93, 492-495 (2010). HPTLC of glycyrrhizin on silica gel with chloroform - methanol - water 130:72:15 in a twin-trough chamber saturated for 30 min. Detection under UV 254 nm and after spraying with anisaldehyde-sulfuric acid reagent. The hRf value of glycyrrhizin was 22. Quantitative determination by densitometry at 254 nm. The linearity was between 0.96-4.80 µg/spot, the correlation coefficient was r = 0.99904 and the standard deviation was 2.52 %. Average recovery was 99.6 %. LOQ and LOD was 246 and 81 ng/spot.

Abstract No. C-453, 61st IPC (2009). Fingerprint analysis of budmunchiamines, the main constituents in Albizia amara. Dried powdered leaves were extracted with petroleum ether (60-80 °C), chloroform, ethyl acetate and 90 % methanol by maceration for 48 h. TLC on silica gel with chloroform - methanol 19:1. Zones 2, 4 and 8 corresponded to the marcocylic alkaloids budmunchiamine A, B, and C, which was confirmed by FTIR, NMR and MS.

Phytochem. Anal. 22, 36-41 (2011). TLC of kutkoside (1) and picroside-I (2) in the kutki extract (Picrorhiza kurroa) on silica gel with ethyl acetate - methanol - glacial acetic acid - formic acid 25:5:1:1. Quantitative determination by absorbance measurement at 265 nm.The hRf of (1) was 42 and of (2) 61. The precision was 0.77 % and 1.01 % for (1) and (2), respectively. Linearity was between 80-480 ng/zone for both substances. Detection and quantification limits were 24 and 79 ng/zone for both. The intra-day and inter-day precisions were 0.4 % and 0.3 % (n=3) respectively. The recovery for (1) was 96.5 % and for (2) 96.0 %, respectively. The results were comparable with those obtained by HPLC.

Acta Chromatographica 22 (3), 481-489 (2010). HPTLC on silica gel with toluene – ethyl acetate – methanol – formic acid 8:10:5:2. The hRf values were 22, 19, and 81 for sennosides A and B and kaempferol, respectively. Quantification by densitometry at 270 nm. The recovery of sennosides A and B and kaempferol from Cassia fistula extract were 98.0, 98.7, and 99.1 %, respectively. The linearity was in the range of 100–400 ng/band. Instrument precision was in the range of 1.03-1.33 % and method precision in the range of 1.3-1.8 %.

International Journal of Pharma and Bio Sciences 1(1), 1-3 (2010). Shade dried aerial parts of the plant were extracted with acetone (A) and methanol (B) and the solvent was removed to get the crude extract. Extract A was further fractioned over silica gel (60-120) by eluting with n-hexane (C) and n-hexane – acetone 9:1( D) . TLC of all fractions on silica gel with n-hexane – ethyl acetate. Quantitative determination by absorbance measurement at 258 nm. The linearity was in the range of 1-5 µg/band. The amount of santonin found in different fractions of the acetone extract was 31.3 mg/g (A), 40.7 mg/g (B), 1.9 mg/g (C), and 20.9 mg/g (D).