Abstract No. F-274, 61st IPC (2009). HPTLC of ondansetron HCl on silica gel with chloroform - ethyl acetate - methanol - 25 % ammonia 90:50:40:1. The hRf value was 52. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the range of 100-1400 ng/band. Recovery was 99.3 %.

60th Indian Pharmaceutical Congress PA-210 (2008). HPTLC of amitriptyline HCl, trifluoperazine HCl, risperidone and alprazolam on silica gel with carbon tetrachloride - acetone - triethylamine 80:20:3. Quantitative determination by absorbance measurement at 250 nm in the range of 50-1200 ng/spot for amitriptyline HCl, 50-1200 ng/spot for trifluoperazine HCl, 100-2400 ng/spot for risperidone, and 25-600 ng/spot for alprazolam. The limit of quantification for amitriptyline HCl and trifluoperazin HCl was 50 ng/spot, for risperidone 100 ng/spot, and for alprazolam 25 ng/spot.

Ind. J. Pharma. Sci. 71(1), 72-74 (2009). HPTLC of valsartan and hydrochlorothiazide on silica gel with chloroform - methanol - toluene - acetic acid 60:20:10:1. Quantitative determination by absorbance measurement at 260 nm. The calibration curve was linear between 300 to 800 ng/spot for valsartan and 100 to 600 ng/spot for hydrochlorothiazide. The limit of detection and the limit of quantification for valsartan were 100 and 300 ng/spot, respectively, and for hydrochlorothiazide 30 and 100 ng/spot, respectively.

J. Planar Chromatogr. 23, 79-83 (2010). HPTLC of anastrozole (2-[3-(1-cyano-1-methyl-ethyl)-5-(1H-1,2,4-triazole-1-yl-methyl)-phenyl]-2-methyl-propanenitrile) on silica gel (prewashed with methanol) with toluene - acetone - ammonia 60:40:3 in a twin-trough chamber previously saturated for 20 min. Quantitative determination by absorbance measurement at 200 nm. Recovery of anastrozole from 1mg tablet formulations ranged from 98.9 to 101.5 %. Intra-day and inter-day precision were 1.34 % and 1.59 %, respectively. LOD and LOQ were 71 and 214 ng/band. The correlation coefficient for the anastrozole calibration was 0.9983 over the range of 500-1500 ng/band (peak area).

J. Planar Chromatogr. 23, 137-140 (2010). HPTLC of aspirin, salicylic acid, and sulfosalicylic acid on silica gel (prewashed with methanol-chloroform 1:1 and impregnated with 2 % boric acid in ethanol) with chloroform - methanol - ammonia - water 120:75:2:6 in a chamber previously saturated at 25 °C for 30 min. Detection and quantitative determination by densitometry at 254 nm. The hRf of aspirin, salicylic acid, and sulfosalicylic acid were 81, 61, and 24, respectively. The linear range was 100-1000 ng/band for all three compounds, and the correlation coefficients r were 0.97, 0.94, and 0.95, respectively. LOQ were 123, 95, and 61 ng/band, respectively, and the respective LOD were 37, 37, and 18 ng/band.

J. Planar Chromatogr. 23, 396-399 (2010). TLC of 8 newly synthesized N-phenyltrichloroacetamides on RP-18 W with micellar-organic solutions composed of 0.02, 0.04, or 0.06 M Brij 35 (2-dodecoxyethanol, a nonionic surfactant) and tetrahydrofuran 4:1 in saturated sandwich chambers. Detection under UV 254 nm. According to the results obtained from the OPLC studies, especially log km values are excellent descriptors of the lipophilicity of the compounds.

J. Planar Chromatogr. 24, 236-241 (2011). HPTLC of levodopa (LEV), carbidopa CAR), and entacapone (ENT) in a combined dosage form on RP-18 (prewashed with methanol) with acetonitrile - n-butanol - water - triethylamine 1:19:2:0.002, pH adjusted to 3.6 with phosphoric acid, in a twin-trough chamber saturated with mobile phase for 25 min. Quantitative determination by densitometry in absorbance mode at 282 nm. The hRf values were 46, 64, and 87 for LEV, CAR, and ENT, respectively. Linearity was between 300-1500 ng/zone for LEV, 200-1000 ng/zone for CAR, and 200-2000 ng/zone for ENT. The intra-day and inter-day precision was below 1.8 % RSD for all drugs. The recovery for LEV, CAR, and ENT (n = 3) was between 101.0 and 102.4 %.

J. Liq. Chromatogr. Relat. Technol. 34, 2606-2620 (2011). HPTLC of dexketoprofen trometamol in pharmaceutical formulations on silica gel with toluene - ethyl acetate 3:1 + 1 drop glacial acetic acid. Quantitative determination by absorbance measurement at 255 nm. The hRf of 1 was 45. Linearity was 20-120 ng/zone. LOD and LOQ were found to be 5 and 10 ng/zone. Repeatability and intermediate precision (%RSD, n = 6) were below 2 %. Recovery (by standard addition) ranged between 98.8 and 99.3 %. The HPTLC method was suitable to determine the purity of the drug available from various sources by detecting the related impurities.