J. Planar Chromatogr. 22, 175-181 (2009). TLC of 14 tetraoxanes on silica gel with ethyl acetate - toluene and ethyl acetate - petroleum ether, on cyano phase with methanol - water and acetone - water, and on RP-18 with water - organic modifier (methanol, acetone, or dioxane) in various combinations, with chamber saturation for 15 min at ambient temperature (22 +/- 2 °C). Detection by spraying with 50 % sulfuric acid, followed by heating until spots became visible.

J. Liq. Chrom. & Rel. Technol. 19, 687-698 (1996). TLC of 3-indoleacetic acid, 5-hydroxytryptophan, serotonin-creatinine sulfate, a-tryptophan on silica gel, cellulose and RP-18; TLC of indolic compounds on cellulose with chloroform - methanol - ammonia 12:7:1, light protected. Quantitation by densitometry at 280 nm (excitation) and 347 nm (emission). Determination of the compounds over the range 10-100 ng, with relative standard deviations between 1.70-6.52 % and detection limits over the range 16.39-22.50 ng.

Ind. J. Pharma. Sci. 8(4), 198-201(2009). HPTLC of nebivolol hydrochloride and valsartan on silica gel with ethyl acetate - methanol - acetic acid 12:2:1 in a twin trough chamber saturated for 15 min. Quantitative determination by absorbance measurement at 240 nm for valsartan and 280 nm for nebivolol hydrochloride. The method was found to be linear in the range of 600-1400 ng/band for valsartan 1200-2800 ng/band for nebivolol. The sample were subjected to different stress conditions (acid, alkali, oxidation, photolysis, thermal) and all degradation products were well separated from the main compounds.

Chromatogr. (Sepu) 20 (2), 182-184 (2002). TLC of the major metabolite of flunitrazepam on silica gel with ethyl acetate - chloroform 2:1. Detection by spraying with fluorescamine reagent, heating at 708C and under UV 365 nm. Detection limit 5 mg/L. Semi quantitation by standard comparison. Quantitation limit 15 mg/L.

Abstract No. F-269, 61st IPC (2009). HPTLC of hydrochlorothiazide and irbesartan on silica gel with acetonitrile - chloroform 5:6. The hRf value was 27 and 45 for irbesartan and hydrochlorothiazide, respectively. Quantitative determination by absorbance measurement at 270 nm. The sample was exposed to different stress conditions (acid, alkali, oxidative, photodegradation, thermal). Neither of the compounds showed degradation under thermal and photodegradation conditions, but both compounds showed significant degradation under acid, alkali and hydrolytic conditions. Degraded products were well resolved from the parent compounds.

Abstract No. F-274, 61st IPC (2009). HPTLC of ondansetron HCl on silica gel with chloroform - ethyl acetate - methanol - 25 % ammonia 90:50:40:1. The hRf value was 52. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the range of 100-1400 ng/band. Recovery was 99.3 %.

60th Indian Pharmaceutical Congress PA-210 (2008). HPTLC of amitriptyline HCl, trifluoperazine HCl, risperidone and alprazolam on silica gel with carbon tetrachloride - acetone - triethylamine 80:20:3. Quantitative determination by absorbance measurement at 250 nm in the range of 50-1200 ng/spot for amitriptyline HCl, 50-1200 ng/spot for trifluoperazine HCl, 100-2400 ng/spot for risperidone, and 25-600 ng/spot for alprazolam. The limit of quantification for amitriptyline HCl and trifluoperazin HCl was 50 ng/spot, for risperidone 100 ng/spot, and for alprazolam 25 ng/spot.

Ind. J. Pharma. Sci. 71(1), 72-74 (2009). HPTLC of valsartan and hydrochlorothiazide on silica gel with chloroform - methanol - toluene - acetic acid 60:20:10:1. Quantitative determination by absorbance measurement at 260 nm. The calibration curve was linear between 300 to 800 ng/spot for valsartan and 100 to 600 ng/spot for hydrochlorothiazide. The limit of detection and the limit of quantification for valsartan were 100 and 300 ng/spot, respectively, and for hydrochlorothiazide 30 and 100 ng/spot, respectively.