Food Chem. 91, 757-764 (2005). HPTLC of dihydrosanguinarine (1), after its conversion to sanguinarine (2) as an index of argemone oil adulteration in edible mustard oil, on silica gel with hexane – acetone – methanol 16:3:1. The plate was irradiated under long wave UV light for 15 min to oxidize (1) to (2). Quantitative determination by absorbance measurement at 366 nm. The hRf values for (1) and (2) were 82 and 36, respectively. Linearity was between 5 and 300 ng/zone for (2). The limit of detection and quantification was 1 and 3 ng/zone. Recovery was between 79 and 82 %.

J. Chromatogr. A 1173 (1-2), 146-150 (2007). HPTLC of oenothein B and quercetin glucuronide in aqueous extract of Epilobii angustifolii herba on RP-18 W phase with 1) 25 % acetonitrile in water (with 50 mM H3PO4) over 80 mm for oenothein B, and 2) with acetonitrile over 40 mm for quercetin glucuronide. Quantification of oenothein B and quercetin glucuronide by densitometry at 270 and 350 nm, respectively. Linearity was between 1.14 and 2.28 µg/zone for oenothein B and 77 and 691 ng/zone for quercetin glucuronide. Aqueous extract of Epilobii angustifolii herba contained 152.46 ± 4.92 mg/g oenothein B and 22.07 ± 1.38 mg/g quercetin glucuronide.

J. AOAC Int. 90, 1203-1209 (2007). HPTLC of terpenelactones (total bilobalide, gingkolide A, and gingkolide B) on prewashed and sodium acetate preimpregnated silica gel with toluene - ethyl acetate - acetone - methanol 50:25:25:3 or ethyl acetate - hexane 9:1; also HPTLC of flavonol glycosides (quercetin, kaempferol, isorhamnetin as standards) on prewashed and preimpregnated silica gel with chloroform - acetone - formic acid - acetic acid 50:11:6:6. Plates were developed in solvent equilibrated, vapor saturated twin-trough chambers at 30°C. Densitometry in absorbance mode at 370 nm (for aglycones) and at 290 nm following a 1 s immersion in acetic anhydride and heating at different temperatures for varying lengths of time (for terpenelactones). Good relationship (95%) was determined between HPTLC and HPLC for determination of total flavonol glycosides. The HPTLC flavonol aglycone method also performed well in terms of accuracy and consecutive plate repeatability.

J. Sep. Sci. 30, 2167-2172 (2007). HPTLC of clozapine in human serum on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 290 nm. Linearity was between 10 and 100 ng/zone. The intra-assay variation was between 2.10 and 3.33 % (n=5) and inter-assay variation was between 2.67 and 4.44 % (n=9). The limits of detection and quantification were 0.03 and 0.05 ng/µL, respectively. Recovery was between 97.0 and 99.0 %, and selectivity regarding matrix was given.

59th Indian Pharmaceutical Congress C-66, 240, (2007). Evaluation of Arjunakhseerpak and Arjunaristha, two ayurvedic formulations used for cardiovascular system, which contain Terminalia arjuna as the main ingredient. Simultaneous HPTLC of arjungenin and arjunolic acid from Terminalia arjuna on silica gel with toluene - ethyl acetate - acetic acid 10:10:1. Detection by spraying with 10 % sulphuric acid followed by heating. Densitometric evaluation at 366 nm. The UV spectra of the sandards arjungenin and arjunolic acid was found to be similar to that of arjungenin and arjunolic acid in the formulation. The method was found suitable for standardization of arjuna formulations.

Food Chem. 79, 337-342 (2002). HPTLC of polyphenol compounds (caffeic acid derivatives, quercetin and kaempferol glycosides) in the leaves of Lactuca sativa on RP-18 with water – methanol – acetic acid 25:25:3. Detection by dipping into a solution of 1 % ethanolamine diphenylborate in methanol for 24 h. Quantitative determination by absorbance measurement at 365 and 440 nm. The total flavonoid amount is expressed as isoquercitrin using a three point regression curve in the range of 1 and 5000 ng/zone.

J. Planar Chromatogr. 20, 437-441 (2007). HPTLC of the 4 biomarkers bergapten, psoralen, rutin, and chlorogenic acid, on silica gel with ethyl acetate - formic acid - acetic acid - water 20:2:2:5 for chlorogenic acid and rutin and with petroleum ether - diethyl ether - acetic acid 5:5:1 for bergapten and psoralen in a saturated twin-trough chamber. Quantitative determination at 317 nm.

J. Planar Chromatogr. 21, 15-20 (2008). TLC and OPLC of polyacetylenes of chamomile, trans-resveratrol, salicylic acid, and ochratoxin A on silica gel (preconditioned at 120 °C for 3 h) with chloroform - methanol 20:1. Detection by bioautography with Pseudomonas savastanoi pv. phaseolicola race 6 and by immersing the humid plate into MTT solution for 5 s.