Chinese J. Trad. Pat. Med. (Zhongchengyao) 26 (9), App.12-14 (2004). TLC on silica gel with 1) n-butanol - glacial - water 19:5:5; 2) chloroform - methanol - glacial acetic acid - 17:2:1; 3) two fold development with benzene - acetone 9:1. Detection 1) by spraying with ninhydrin solution and heating at 105 ºC; 2) by spraying with 5 % vanillin solution and heating; 3) by spraying with a solution of 8 % vanillin in ethanol - H2SO4, and heating at 105 ºC. Identification by fingerprint technique. Quantification of phyllyrin by densitometry at 280 nm. The quantitative procedure is validated by investigating its linearity (1 - 5 µg/spot, r = 0.9998); precision (RSD = 0.36 % n = 5); repeatability (RSD = 2.92 % n = 5) and standard addition recovery (99.6 %, RSD = 2.4 %, n = 5), etc. The determination results are given for a group of real life samples.

CBS 95, 14-15 (2005). HPTLC-AMD of lipids from drug formulations with an 11-step gradient based on ethyl acetate. Detection by dipping in an aqueous copper sulfate solution followed by heating at 150 °C for 30 min. Quantitative determination by absorbance measurement at 675 nm, evaluation of peak area with calibration according to Hill kinetics.

J. Planar Chromatogr. 18, 39-43 (2005). Micropreparative TLC of 10 pesticides (isoproturon, diuron, momolinuron, desmetryn, methiocarb, atrazine, fenitrothion, terbutryn, bromopropylate, aziprotryne) for preliminary fractionation on silica gel in horizontal DS chambers with tetrahydrofuran - n-heptane 1:4; detection under UV at 254 nm. HPTLC of the separated fractions on RP-18 W with methanol - water 3:2 and acetonitrile - water 3:2; densitometry at 254 nm.

Abstract G-26, IPC (2005). A simple HPTLC method is reported for analysis of guggulsterones E and Z in herbal extract and market formulations containing commiphora mukul. Guggulsterones were extracted from crude extract and formulations by ethyl acetate. HPTLC on silica gel with n-hexane - ethylacetate 3:1. Quantitative determination by absorbance measurement at 250 nm. The hRf value of E-guggulsterone was 38 and of Z-guggulsterone 46, linearity range for both isomers was 200-5000 ng/mL. The method was validated as per ICH guidelines.

Abstract G-19, IPC (2005). HPTLC for estimation of corticosterone in rat plasma on silica gel with chloroform - methanol - water 9:10:1. Quantitative determination by absorbance measurement at 245 nm. Betamethasone was employed as internal standard. The extraction of plasma with ethyl acetate gave an average recovery of >85 %. The linearity was within the range of 30-300 ng/mL with LOQ being 30 ng. The method was found to be rugged and robust.

J. Pharm. Biomed. Anal. 39, 801-804 (2005). HPTLC on silica gel with chloroform - methanol 9:1. Quantitative determination by absorbance measurement at 265 nm. The hRf values of stavudine (SV), lamivudine (LV) and nevirapine (NV) were 25, 67 and 87 respectively. The method was linear within the range of 0.01-0.06 mg/mL, 0.05-0.30 mg/mL and 0.06-0.40 mg/mL for SV, LV, and NV respectively, recovery rates were between 98.2 and 99.9 %. The HPTLC method was compared with the UV and HPLC methods. Accuracy, precision and ruggedness of the method were established.

J. Planar Chromatogr. 18, 294-299 (2005). TLC of (+/-)-metoprolol tartrate and (-)-alprenolol tartrate on silica gel, with concentrating zone, on RP-18, on LiChrospher silica gel and on alumina, previously impregnated with the mobile phase (ethanol - water 7:3, containing D-(-)-tartaric acid as chiral selector). Detection under UV light at 254 nm and densitometric scanning at 230 nm. Direct separation of the enantiomers of (+/-)-metoprolol tartrate was achieved.

CBS 96, 6-7 (2006). HPTLC of oxytetracycline in salmon feed, on silica gel (pre-washed with methanol and dried at 120 °C for 30 min, followed by dipping into 5 % EDTA solution of pH 7.0 and drying at 120 °C for 1 h in an oven) with the organic layer of dichloromethane - methanol - 5 % EDTA 13:4:2 with chamber saturation for 30 min.Quantitative determination by fluorescence measurement at UV 366/>400 nm. Calibration (peak area) was performed via linear regression with r2 of 0.9925. Recovery rates for oxytetracycline at 500, 2500, and 5000 mg/kg were 73 ± 4.2 %, 101 ± 2.6 %, and 101 ± 4.0 %. Intermediate precisions at the same levels were 5.7, 2.6 and 4.0 %. At an application volume of 10 µL LOD was 14.8 mg/kg (n=3) and LOQ was 49.2 mg/kg (n=10). Quantification was achieved between 100 and 10000 mg/kg oxytetracycline in salmon feed due to the selectivity of fluorescence measurement.