Abstract No. F-238, 61st IPC (2009). HPTLC of salbutamol silphate and guaiphenesin, used as pharmaceutical syrup against cough, on silica gel with ethyl acetate - methanol - 25 % ammonia 15:3:2. The hRf value was 47 and 65 for salbutamol and guaiphenesin, respectively. Quantitative determination by absorbance measurement at 280 nm. The method was linear in the range of 200-1000 ng/band for salbutamol and 10-15 µg/band for guaiphenesin.

Abstract No. F-251, 61st IPC (2009). HPTLC of doxazosin mesylate on silica gel with acetone - toluene - 25 % ammonia 60:40:1. The hRf value was 65. Quantitative determination by absorbance measurement at 251 nm. Linearity was in the range of 20-100 ng/band. Recovery was 103.3 %.

Abstract No. F-271, 61st IPC (2009). HPTLC of telmisartan and rampril on silica gel with methanol - chloroform 1:6. The hRf value was 38 and 68 for rampril and telmisartan, respectively. Quantitative determination by absorbance measurement at 210 nm. The sample was exposed to different stress conditions (acid, alkali, oxidative, photo degradation and thermal). Both drugs did not degrade under acidic and photolytic conditions, but showed significant degradation under alkaline and thermal conditions. Both compounds were well separated from different degradation products under experimental conditions.

Abstract No. F-236, 61st IPC (2009). HPTLC of pantoprazole and mosapride citrate on silica gel (pre-washed with methanol) with ethyl acetate - benzene - methanol - 25 % ammonia 48:35:15:2 at room temperature. Quantitative determination by absorbance measurement at 250 nm or 276 nm. The method was linear in the range of 3-7 µg/band for both drugs. Recovery was between 99.0 and 103.5 %.

J. Planar Chromatogr. 22, 273-276 (2009). HPTLC of bicalutamide and leflunomide (as internal standard) on silica gel with toluene - ethyl acetate 4:5 in a twin trough chamber saturated for 30 min at room temperature. Quantitative determination by absorbance measurement at 273 nm. The limit of detection and quantification was 50 and 200 ng/band, respectively.

J. Pharm. Biomed. Anal. 47, 841-846 (2008). HPTLC of iridoid glycosides in the aerial part of Gambhari (Gmelina arborea) with iridoid gycoside 6-0-(2”, 3”- dibenzoyl)-o-L-rhamnopyranosylcatalpol as a chemical marker for the standardization of G. arborea plant extracts on silica gel with chloroform - methanol 4:1. Quantitative determination by absorbance measurement at 240 nm and at 430 nm after derivatization with vanillin - sulfuric acid reagent. The linear working range was between 1000-5000 ng/spot with a good correlation coefficient of 0.994.

Quim. Nova 33, 43-47 (2010). HPTLC of aflatoxin B1 in peanuts on silica gel with chloroform - acetone 99:1. Quantitative determination by absorbance measurement at 366 nm, using a CCD camera followed by image processing using the software ImageJ. Linearity was between 0.8 and 4.8 ng/zone. The intra-day and inter-day precisions had a RSD lower than 5.2 %. LOD was 0.4 ng/zone while LOQ was 1.2 µg/kg. The average recovery was 94.9 %. The proposed method is a simple, efficient and low cost tool for quantitative analysis of aflatoxin B1 in peanut samples.

J. AOAC Int. 93, 1617-1621 (2010). HPTLC of aconitine on silica gel with ethyl acetate - ethanol 3:1 at 22 °C in a saturated twin-trough chamber. The hRf value of aconitine was 33. Quantitative determination by absorption measurement at 238 nm. LOD and LOQ was 20 and 70 ng/band, respectively. The linearity with respect to peak area was in the range of 300 to 1800 ng/band with an r of 0.9991. The repeatability (RSD) was 0.85 %; and the inter-day and intra-day precision (RSD) was 1.01-1.38 and 1.04-1.34 %, respectively.