Chinese J. of Today’s Pharm. 22 (11), 659-663 (2012). Rulekang gel ointment is a herbal TCM for treating diseases of the mammary glands. For quality control, TLC on silica gel 1) for Nidus vespae, with n-hexane – chloroform – ethyl acetate 16:3:4, detection under UV 366 nm; 2) for Olibanum and Myrrh, with toluene – ethyl acetate 19:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing in daylight; 3) for Syzygium aromaticum, with petroleum ether ( 60-90 °C) – ethyl acetate 8:1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing in daylight; 4) Rhizoma Curcumae Longae, with chloroform - methanol – formic acid 96:4:1, detection by viewing in daylight; 5) for Herba Asari, with n-hexane – ethyl acetate 4 1, detection by spraying with 5 % vanillin in sulfuric acid – ethanol 1:4 and heating at 105 °C, viewing under UV 366 nm; 6) for Fructus Piperis Longi, with n-hexane – ethyl acetate – formic acid 600:400:1, detection under UV 366 nm. Quantification of curcumin by HPLC.

J. Planar Chromatogr. 27, 29-32 (2014). HPTLC of rhein in the pulp of Cassia fistula on silica gel with ethyl acetate - methanol - water 100:17:10. Quantitative determination (1) by TLC image analysis using a flatbet scanner and (2) by absorbance measurement at 435 nm. The hRF value for rhein was 49. Linearity was in the range of 400-1200 ng/zone for (1) and 29-230 ng/zone for (2). The intermediate/interday/intra-day precisions were below 3 % (n=3). The LOD and LOQ for rhein were 85 and 257 ng/zone for (1) and 3 and 11 ng/zone for (2). Mean recovery was 102.3 % for (1) and 101.2 % for (2).

J. Planar Chromatogr. 27, 2980-2988 (2014). HPTLC of piperine in commercial samples of pepper on silica gel with acetone - n-hexane 3:2. Quantitative determination by absorbance measurement at 360 nm. The hRF value for piperine was 90. The LODs and LOQs were 590 and 1800 ng/zone, respectively. Recoveries were between 98.1 and 99.2 %

J. Liq. Chromatogr. Relat. Technol. 37, 1568-1582 (2014). HPTLC of artesunate (1) and amodiaquine (2) on silica gel with acetonitrile – water – ammonia 40:7:2. Quantitative determination by absorbance measurement at 366 nm. The hRf values for (1) and (2) were 44 and 50, respectively. Linearity was in the range of 100-600 ng/zone for (1) and 50-300 ng/zone for (2). The intermediate/interday/intra-day precisions were below 2 % (n=9). The LOD and LOQ were 25 and 60 ng/zone for (1) and 12 and 30 ng/zone for (2), respectively. Recovery was 98.1-99.9 % for (1) and 99.3-100.9 % for (2).

J. Planar Chromatogr. 28, 229-233 (2015). HPTLC of paroxetine in human serum on silica gel with toluene - acetone - ethanol - ammonia 25 % in water 9:5:2:1. Quantitative determination by absorbance measurement at 294 nm. Linearity was between 0.01 and 0.14 ng/µL. The intra-day precisions were between 0.9 % and 2.2 % (n=5) and the inter-day precisions were between 1.0 % and 3.9 % (n=9). The LOD and LOQ was 0.3 and 0.6 ng/zone, respectively. Recoveries were between 91.5 % and 96.5 %.

J. Chromatogr. Sci. 54 (1), 58-63 (2016). Description of a method for simultaneous determination of the two monoterpenes menthol and eucalyptol, contained in several analgesic pharmaceutical formulations for external use, by HPTLC on silica gel with hexane – ethyl acetate 4:1. The hRF value of menthol was 34 ± 4 and of eucalyptol 56 ± 4. Linearity was between 100–800 ng/zone (r2 = 0.9979) for menthol and 52–1107 ng/zone (r2 = 0.9937) for eucalyptol.

CBS 118, 1-4 (2017). Screening of steroids and selective androgen receptor modulators (SARMs) in bodybuilding supplements by HPTLC of methanedienone, ibutamoren, and ostarine on silica gel with n-heptane – ethyl acetate 1:1 (steroids) and dichloromethane – methanol 9:1 (SARMs) to the migration distance of 70 mm. Detection by immersion into (1) toluene sulfonic acid reagent (10 % in ethanol) followed by heating at 150 °C for 3 min, or (2) Seebach reagent (5 g phosphomolybdic acid, 2 g cerium sulfate and 12 mL sulfuric acid made up to a volume of 200 mL with water) followed by heating at 110 °C for 5 min. Detection under UV 254 nm, 366 nm and under white light. Densitometric evaluation by multi-wavelength scan at the respective absorption maxima. For example for the steroid testosterone the LOD was 8 ng/zone (absorbance measurement at 250 nm). Direct elution of target zones into the MS and analysis in positive ionization mode.

J. Planar Chromatogr. 31, 122-128 (2018). HPTLC of ondansetron with chloroform ‒ methanol ‒ ethyl acetate 7:2:1. Quantitative determination by absorbance measurement at 302 nm. The hRf value for ondansetron was 67. Linearity was in the range of 25-500 ng/zone. The intermediate precision was below 1 % (n=3). The LOD and LOQ were 5 and 15 ng/zone, respectively. Average recovery was 100.3 %. The method allowed the separation of the drug from its different degradation products.