J. Liq. Chromatogr. Relat. Technol. 31, 1204-1212 (2008). HPTLC of mirtazapine (1,2,3,4,10,14b-hexahydro-2-methyl-pyrazino[2,3-c][2-benzazepine]), and three impurities (2-(4-methyl-2-phenyl-piperazin-1-yl)nicotinic acid, [2-(4-methyl-2-phenyl-piperazinyl)-pyridin-3-yl]methanol, and 2-chloronicotinic acid on silica gel with toluene - acetone - methanol 6:2:2 with chamber saturation. Quantitative determination by absorbance measurement at 285 nm. The limit of detection and quantification for mirtazapine was 22 and 75 ng/spot, respectively.

J. Liq. Chromatogr. Relat. Technol. 31, 1892-1902 (2008). HPTLC of hydrochlorothiazide, walsartan, kandesartan, and enalapril on silica gel after chamber saturation using ethyl acetate - tetrahydrofuran - acetic acid 16:4:1 (for kandesartan and walsartan present together with hydrochlorothiazide) and 1-butanol - acetic acid - water 12:3:5 (for enalapril and hydrochlorothiazide). Quantitative determination by absorbance measurement at 252 nm for walsartan and kandesartan, at 274 nm for hydrochlorothiazide and at 208 nm for enalapril. The method was of high sensitivity and specific to analyte constituents.

Acta Chromatographica 9, 79-86 (1999). HPTLC (TLC) of glucose, maltose, sucrose and trehalose on silica gel with acetonitrile - water 17:3 and ethyl acetate – acetic acid – methanol – water 12:3:3:2 for three times with chamber saturation; on amino plates with ethyl acetate – pyridine – water – acetic acid 12:6:2:1 and on cellulose plates with ethyl acetate – pyridine – water 2:1:2, both in a non-equilibrated chamber; on RP-18 with tetrahydrofuran – water 44:6 in a pre-equilibrated chamber. All plates were prewashed, e.g. by chromatography with dichloromethane - methanol 1:1. Detection by 4-aminobenzoic acid reagent, 1-naphthol–sulfuric acid reagent or aniline–DPA reagent, each followed by heating at 110 °C for 10 min. The combined results from comparison of hRf values for two different mobile phases on silica gel, on cellulose, amino phase and C18-bonded layers with diverse separation mechanisms, spiking experiments on silica gel, detection with selective reagents, and GC–MS analysis definitely proved the presence of maltose and glucose and the absence of trehalose in digestive gland–gonad complex and hemolymph samples from B. glabrata. Earlier papers reporting the presence of trehalose were undoubtedly in error.

Part I: Graft TLC of selected coumarins. J. Planar Chromatogr. 21, 237-241 (2008). HPTLC of coumarins (present in extracts of Archangelica officinalis, Pastinaca sativa and Heracleum sphondylium fruits) on cyano phase with acetonitrile - water 3:7 (triple development) in the first dimension, and on silica gel with ethyl acetate - n-heptane 7:13 (triple development) in the second dimension. Good selectivity differences were also obtained on silica gel with ethyl acetate - n-heptane 7:13 (triple development) in the first dimension and on RP-18 phase with methanol - water 11:9 (triple development) in the second dimension. Detection and quantitative determination by fluorescence measurement at 366 nm.

Abstract No. 0983, IHCB (2009). HPTLC of mahanimbine (a carbazole alkaloid isolated from Murraya koenigi) on silica gel with petroleum ether - chloroform 13:7. The hRf value of mahanimbine was 60. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the concentration range of 50-250 ng/spot. Enzyme inhibition activity of methanol, petroleum ether, and chloroform exctracts of the plant and of mahanimbine was evaluated using galantamine as standard inhibitor of the enzyme.

Chromatographia 69 (5-6), 597-599 (2009). TLC of karanjin in the seeds of Pongamia pinnata on silica gel with toluene - ethyl acetate 7:3. Quantitative determination by absorbance measurement at 260 nm. The limit of detection was 100 ng, linearity was 50 - 300 ng. Four samples of P. pinnata from different geographical locations were screened for their karanjin content.

J. Planar Chromatogr. 21, 349-353 (2008) TLC of S-(+)-ketoprofen on silica gel (prewashed by development with methanol - water 9:1 and impregnated by dipping for 2 s in a solution of L-arginine in methanol) at 22 +/- 2 °C with acetonitrile - water 5:1 acidified with several drops of glacial acetic acid (to maintain the pH <4.8) in one-dimensional and two-dimensional modes. Quantitative determination by absorbance measurement at 252 nm.

Acta Chromatographica 21 (1), 29-38 (2009). Evaluation of different TLC systems for analysis of 21 amino acids in biological tissues and fluids. Detection by derivatization with ninhydrin reagent, and determination of Rf values by slit-scanning densitometry. The five most suitable systems were cellulose and silica gel plates developed with either 2-butanol - pyridine - acetic acid - water 39:34:10:26 or 2-butanol - pyridine - 25 % ammonia - water 39:34:10:26, and ion exchange plates developed with citrate buffer of pH 3.3. Detection with ninhydrin allowed the identification of all amino acids except for leucine and isoleucine in complex mixtures. Quantification is possible as well if the amino acid of interest is well separated from adjacent components of the mixture. The method is illustrated with example chromatograms on cellulose HPTLC layer showing the identification and separation of amino acids in snail tissue samples.