Phytochem. Anal. 21, 556-560 (2010). HPTLC of betulinic acid in the rhizomes of Nelumbo nucifera on silica gel with chloroform - methanol - formic acid 49:1:1. Detection by spraying with anisaldehyde - acetic acid - methanol - sulfuric acid 5:100:850:50 and drying at 50 °C for 10 min. Quantitative determination by absorbance measurement at 420 nm. The hRf of betulinic acid was 30. Linearity was between 2 and 10 µg/zone. Detection and quantification limits were 0.4 and 2.30 µg, respectively. The intra-day and inter-day precisions were 0.4 % and 0.3 % (n=3) respectively. Recovery (by standard addition) was 98.4 %.

Acta Chromatographica 22 (3), 391-404 (2010). Examination of the effect of conditioning of the silica layer by mobile phase vapor, diethylamine vapor and its aqueous and methanolic solutions, and ammonia vapor on the retention of alkaloids eluted with multicomponent non-aqueous mobile phases. Investigation of the effect of conditioning time and vapor phase composition on system efficiency and peak symmetry, and as well the effect of vapor phase composition on separation selectivity.

J. Ethnopharmacol. 134, 992-995 (2011). HPTLC of fumaric acid and fumaric acid conjugates (as dimethyl fumarate) in the aerial parts of Fumaria indica on silica gel with formic acid – chloroform – butanol – heptane 3:4:8:11. Quantitative determination by absorbance measurement at 260 nm.

62nd Indian Pharmaceutical Congress Abstract No. F-290 (2010). TLC of ranolazine on silica gel with methanol – 10 mM ammonium acetate 3:2. The hRf value was 54. Quantitative determination by absorbance measurement at 271 nm. The recovery was 99.9 %.

62nd Indian Pharmaceutical Congress Abstract No. F-250 (2010). TLC of risperidone on silica gel with methanol – ethyl acetate 4:1. The hRf value was 34. Quantitative determination by absorbance measurement at 254 nm. The method was linear in the range of 100-600 ng/band. The proposed method was employed for estimation of solubility equilibrium, analysis of mucoadhesive microemulsion formulations and in vitro diffusion studies.

Acta Chromatographica 22 (3), 481-489 (2010). HPTLC on silica gel with toluene – ethyl acetate – methanol – formic acid 8:10:5:2. The hRf values were 22, 19, and 81 for sennosides A and B and kaempferol, respectively. Quantification by densitometry at 270 nm. The recovery of sennosides A and B and kaempferol from Cassia fistula extract were 98.0, 98.7, and 99.1 %, respectively. The linearity was in the range of 100–400 ng/band. Instrument precision was in the range of 1.03-1.33 % and method precision in the range of 1.3-1.8 %.

J. Trad. Chinese Veterinary Med. (5), 53-55 (2010). TLC on silica gel with acetone – ethanol – hydrochloric acid 10:6:1. Detection by spraying with bismuth potassium iodide – 1 % FeCl3 in ethanol 5:1 and heating at 100 ºC. Quantitative determination of stachydrine by absorbance measurement at 510 nm. The precision was 3.7 %RSD within plate (n=8), and the stability of the measurement within 120 minutes was 4.5 %RSD (n=5). The linearity range was 3.2-38.3 µg/zone (r=0.997, n=6) and standard addition recovery was 96.6 % (RSD=2.0 %, n=6).

Journal of Environmental Science and Health, Part B 46, 557-568 (2011). Review on techniques and applications of TLC and HPTLC for separation, detection, qualitative and quantitative determination and preparative isolation of pesticides. Covered are sample preparation techniques, stationary phases, sample application, mobile phases, development methods using different chambers, detection under UV or by derivatization with various reagents, identification based on hRf values or by online HPTLC-MS, quantification by scanning densitometry or videodensitometry, preparative layer chromatography and thin-layer radiochromatography. Various applications are described. In the review period especially forensic analyses of human and animal samples for pesticides were numerous. The identification and quantification of components from plant extracts with pesticide activity is also reviewed and it is expected that this area will be especially active in the future given the large amount of ongoing worldwide research on phytochemical compounds.