60th Indian Pharmaceutical Congress PA-215 (2008). HPTLC of ranitidine HCl and domperidone in combined dosage form on silica gel with ethyl acetate - methanol - ammonia 100:10:1 in a twin trough chamber saturated for 10 min. Quantitative determination by absorbance measurement at 285 nm. The method was linear in the range of 100-500 ng/µL for both compounds with a recovery of 102.5-100.8 %.

Abstract No. F-239, 61st IPC (2009). HPTLC of hydrochlorothiazide, ramipril and telmisartan on silica gel with ethyl acetate - chloroform - methanol 6:3:1. Quantitative determination by absorbance measurement at 215 nm. The method was suitable for routine quality control of combined formulations.

J. Pharma. Research 8(4), 187-191 (2009). HPTLC of abacavir sulphate and lamivudine on silica gel with methanol - acetone - n-butyl acetate 1:1:2. Quantitative determination by absorbance measurement at 284 nm. The hRf value of abacavir sulphate was 58 and of lamivudine 35. Linearity of abacavir sulphate and lamivudine was in the range of 240-1200 ng/spot and 120-600 ng/spot, respectively. The limit of detection and quantification of abacavir sulphate was 0.7 and 2 ng/spot, respectively, and of lamivudine 1 and 3 ng/spot, respectively.

J. Planar Chromatogr. 22, 297-300 (2009). HPTLC of chlorogenic acid and of plant extracts on silica gel with ethyl acetate - formic acid - water 10:2:3 and ethyl acetate - formic acid - acetic acid - water 100:11:11:21 in a saturated horizontal chamber. Quantitative determination by absorbance measurement at 320 nm. Qualitative detection by derivatization with natural products reagent (1 % in methanol) followed by treatment with 5 % PEG 400 in ethanol.

Abstract No. 9452, IHCB (2009). HPTLC of capsaicin on silica gel with toluene - ethyl acetate 3:2. The hRf value of capsaicin was 38. Quantitative determination by absorbance measurement at 280 nm. The method was linear in the range of 100-1000 ng/spot. Capsaicin was subjected to different stress conditions (alkali, acid, oxidation, thermal). The method is suitable for separation of capsaicin from its degradation products and can be used to indicate stability.

J. Planar Chromatogr. 23, 190-192 (2010). After sample application as bands and fully off-line OPLC separation followed by drying, the separated bands were compressed bilaterally, in parallel, perpendicular to the direction of development, by use of a strong eluent and capillary driven chromatography. To introduce the eluent for band compression onto the layer a simple manual tool equipped with parallel foam strips was constructed. OPLC of a black tea leaf extract and caffeine, theophylline, and theobromine on silica gel (prewashed with acetonitrile - water 17:3) with toluene - acetic acid 3:2. Quantitative determination by densitometry at 280 nm. After band compression 20 ng/zone theophyllin and theobromine could be detected by densitometry in the xanthine standard mixture at a loading of 20 µg/10 mm. These compounds were not visible in the original, uncompressed chromatogram.

J. Planar Chromatogr. 23, 79-83 (2010). HPTLC of anastrozole (2-[3-(1-cyano-1-methyl-ethyl)-5-(1H-1,2,4-triazole-1-yl-methyl)-phenyl]-2-methyl-propanenitrile) on silica gel (prewashed with methanol) with toluene - acetone - ammonia 60:40:3 in a twin-trough chamber previously saturated for 20 min. Quantitative determination by absorbance measurement at 200 nm. Recovery of anastrozole from 1mg tablet formulations ranged from 98.9 to 101.5 %. Intra-day and inter-day precision were 1.34 % and 1.59 %, respectively. LOD and LOQ were 71 and 214 ng/band. The correlation coefficient for the anastrozole calibration was 0.9983 over the range of 500-1500 ng/band (peak area).

J. Ethnopharmacol. 130, 267-271 (2010). HPTLC of evodione and leptonol from the leaves and influorescences of Melicope lunu-ankenda on silica gel with chloroform - methanol 1:1. Detection by spraying with anisaldehyde - sulfuric acid reagent, followed by heating at 105 °C for 5 min. Quantitative determination by absorbance measurement at 580 nm.