J. AOAC Int. 86, 935-940 (2003). TLC of indapamide (4-chloro-N-(2-methylindolin-1-yl)-3-sulfamoylbenzamide), its degradation product, and related substance 2-methylnitrosoindoline on silica gel using toluene - ethyl acetate - glacial acetic acid 69:30:1 after pre-saturation for 1 h. Detection under UV light at 254 nm. Quantitative determination at 424 nm. Limit of detection 0.11 µg/spot. The method was validated according to the guidelines of the USP.

J. Liq. Chrom. Rel. Technol. 27, 2047-2056 (2004). TLC of bromhexin on silica gel with n-butanol - glacial acetic acid - water 260:77:75 in a twin-trough chamber after at least 3 h of saturation. Quantitative determination at 325 nm by absorbance measurement. The method is selective, precise, and accurate and can be used for routine analysis of pharmaceutical preparations in pharmaceutical industry quality control laboratories.

J. Planar Chromatogr. 17, 261-263 (2004). HPTLC of aglycones and glycosides (flavone, hesperetin, naringenin, apigenin, kaempferol, quercetin, myricetin, tiliroside, apigenin 7-glucoside, myricitrin, kaempferol 3,7-dirhamnoside, hyperoside, hesperidin, rhoifolin, rutin, naringin) on silica gel by using mixtures of dichloromethane and ethyl acetate for aglycones and mixtures of ethyl acetate and formic acid for glycosides. The plates were conditioned for 15 min and developed face down in a horizontal chamber. To separate mixtures of flavonoids two-step gradient devlopment was used. In the first step (for gycosides) the plates were developed to a distance of 65 mm with ethyl acetate - formic acid - water 170:30:1. In the second step (for aglycones ) the plates were developed to a distance of 95 mm with dichloromethane - ethyl acetate - formic acid 170:30:1. Quantitation by densitometry at 254 nm.

J. Planar Chromatogr. 17, 479-482 (2004) HPTLC of beta-boswellic acid (BA), acetyl-beta-BA, keto-BA, and acetyl-keto-BA and ethanolic extracts of olibanum resin on silica gel with toluene - ethyl acetate - formic acid - heptane 80:20:3:10 in a twin trough chamber without chamber saturation. Quantitative determination by reflectance measurement at 245 and 285 nm. Also two dimensional development with the same mobile phase in the second direction.

Densitometric determination of mexiletine hydrochloride in capsules. J. Planar Chromatogr. 17, 213-217 (2004). TLC of disopyramide, flecainide, mexiletine, tocainide, and verapamil on aluminium oxide and silica gel in horizontal chambers. The best mobile phase for separation on the alumina plates was tetrahydrofuran - hexane - 25 % ammonia 25:24:1 and on silica chloroform - tetrahydrofuran - ethanol - 25 % ammonia 81:19:20:1. Detection under UV light at 210 nm and by use of different reagents. Quantification of mexiletine hydrochloride in capsules was performed densitometrically at 254 nm.Correlation coefficient in the concentration range 20 - 45 µg per band was 0.9974, with RSD of 5.23 %.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27 (2), 225-227 (2004). TLC on silica gel with 1) petroleum ether (30 - 60 ºC) - ethyl acetate 9:1; 2) benzene - glacial acetic acid 4:1. Detection 1) by spraying with 1 % vanillin solution and heating at 105 ºC for 10 min; 2) under UV 365 nm. Identification by fingerprint technique. Quantification of ferulic acid by densitometry at 325 nm. Validation of the method by investigation of linearity (0.16 µg - 1.6 µg, r = 0.9992); precision (RSD = 1.8 %, n= 5 within plate and RSD = 2.3 % plate to plate); reproducibility of five time assay towards the same sample (RSD = 0.5 %); and standard addition recovery (97.17 %, RSD = 0.9 %, n = 5). The results for three real life samples are given.

J. Planar Chromatogr. 18, 23-27 (2005). HPTLC-AMD of fructo-oligosaccharides and inulin mixtures (sucrose, 1-kestose, nystose, and fructosylnystose) on diol phases at 55-65 % relative humidity in a twin-trough chamber with an acetonitrile - acetone - water polarity gradient. Detection by derivatization with 4-aminobenzoic acid reagent and quantitation by scanning at 366 nm.

J. Planar Chromatogr. 18, 28-33 (2005). HPTLC and TLC of four carbamate residues (pirimicarb, methomyl, carbofuran, carbaryl) in vegetables on silica gel (prewashed with chloroform - methanol 1:1 followed by drying at 110 °C for 30 min) with system I (two fold development with first toluene - acetone 4:1, and second dichloromethane - acetone 4:1), and system II (two fold development with first ethyl acetate - petroleum ether 3:2, and second chloroform - petroleum ether 9:1). Quantitative determination by densitometric scanning at 254 and 366 nm.