Chinese J. Pharm. Anal. 28 (9), 1554-1556 (2008). TLC of scalreol on silica gel with n-hexane – ethyl acetate – formic acid 32:16:1. Detection by spraying with vanillin – sulfuric acid – ethanol 1:1:18. Quantification by densitometry at 520 nm. Linearity was between 10 and 50 µg/zone with a determination coefficient of 0.9994. Recovery was 100.1 % (n = 6, %RSD = 0.9). Repeatability (%RSD, n = 6) was 2.1 % within plate and 2.3 % plate-to-plate.

Ind. J. Pharma. Sci. 71(1), 72-74 (2009). HPTLC of valsartan and hydrochlorothiazide on silica gel with chloroform - methanol - toluene - acetic acid 60:20:10:1. Quantitative determination by absorbance measurement at 260 nm. The calibration curve was linear between 300 to 800 ng/spot for valsartan and 100 to 600 ng/spot for hydrochlorothiazide. The limit of detection and the limit of quantification for valsartan were 100 and 300 ng/spot, respectively, and for hydrochlorothiazide 30 and 100 ng/spot, respectively.

J. Planar Chromatogr. 22, 399-403 (2009). TLC of celecoxib, etoricoxib, and valdecoxib on silica gel with chloroform - acetone - toluene 12:5:2 with chamber saturation for 15 min at room temperature. Quantitative determination by absorbance measurement at 254 and 290 nm.

J. Planar Chromatogr. 22, 421-424 (2009). TLC of domperidone and paracetamol on silica gel with acetone - toluene - methanol 2:2:1 in a twin trough chamber saturated for 30 min at room temperature. Quantitative determination by absorbance measurement at 285 nm for domperidone and at 248 nm for paracetamol.

J. Planar Chromatogr. 23, 193-197 (2010). HPTLC of ochratoxin A, aflatoxin B1, G1, B2, and G2 on silica gel with t-butyl methyl ether - water - methanol - cyclohexane 48:1:2:1 in an unsaturated horizontal developing chamber and on RP18 with methanol - 4 % aqueous zinc sulfate solution - ethyl methyl ketone 5:5:1. After development the silica gel plate was dipped for 2 s in silicone oil - hexane 1:2 which enhanced aflatoxin fluorescence by a factor of 2 and ochratoxin A fluorescence by a factor of 3-10. RP18 plates were developed to a distance of 75 mm in an unsaturated vertical chamber. Averaged densitograms were obtained in the emission wavelength range from 445 to 485 nm. Sample pretreatment was by modified QuEChERS (Quick, Easy; Cheap, Effectice, Rugged, Safe) extraction with tetahydrofuran or acetone. Linearity was in the range of 3 to 100 pg/zone for aflatoxins B2 and G2, 10 to 350 pg/zone for aflatoxins B1 and G1, and 0.25 to 2.5 ng/zone for ochratoxin A. LOQ for the aflatoxins were between 13 and 35 pg/zone (equivalent to to 1.5 and 2.5 ppb); for ochratoxin A it was 970 pg/zone (56 ppb).

Abstract No. F-159 61st IPC (2009). HPTLC of telmisartan and amlodipine besylate on silica gel with tetrahydrofuran - dichloroethane - methanol - 25 % ammonia 30:10:5:2. Both compounds were well resolved with hRf values of 22 and 45 for telmisartan and amlodipine besylate respectively. Densitometric evaluation at 326 nm. The method was found to be linear in the range of 1200-7200 ng/band for telmisartan and 400-1400 ng/band for amlodipine besylate.

Indian Drugs 47(2), 71-74 (2010). HPTLC of irbesartan and hydrochlorothiazide on silica gel with acetone - chloroform - ethyl acetate - methanol 6:6:6:1. The plates were preconditioned for 10 min in a saturated chamber prior to development. The hRf value of irbesartan was 27 and of hydrochlorothiazide 37. The linearity was 1500-9000 ng/band and 125-750 ng/band for irbesartan and hydrochlorothiazide respectively. The average recovery for both drugs was 99.4-99.5 %.

Abstract No. C-37, 61st IPC (2009). HPTLC of methanolic leaf extracts of Aloe vera (after purification with petroleum ether (60-80 °C)) on silica gel with toluene - ethyl acetate - glacial acetic acid - methanol 4:18:1:4. Under UV 254 nm six bands with hRf values of 12, 26, 34, 44, 62, and 84 were observed. These bands correspond to well known constituents of Aloe vera: aloeresin hRf 25, barbaloin hRf 33, aloe emodin hRf 43, emodin hRf 63. The bands with hRf values of 12 and 84 could not be identified. The reported finger print profiling can serve as potential technique for authentification and batch to batch consistency of herbal drugs.