Abstract D-12, IPC (2005). HPTLC of methanol and ethyl acetate extracts of trigonella foenum graecum on silica gel with n-propanol - ammonia 11:9. Detection by spraying with ninhydrin reagent. Quantitative determination by absorbance measurement and in visible range. The hRf value of isoleucin was 60. Methanolic extracts contained 0.17 % isoleucin and ethyl acetate extracts 0.008 %. Accuracy, precision, linearity of the method were established.

Indian J. Pharm Sciences 67 (6), 687-690 (2005). HPTLC of sparfloxacin extracted with dichloromethane from plasma. A standard solution was prepared in methanol - dichloromethane 1:1. HPTLC on silica gel with chloroform - toluene - methanol - diethylamine 44:15:2:1. Quantitative determination by absorbance measurement at 301 nm. The method had a linearity range of 80-200 ng/spot with an average recovery of 89.17 %.

CBS 96, 2-5 (2006). AMD-HPTLC of amitrol in water samples, on LiChrospher silica gel pre-washed by immersion for 8 h in 1 % formic acid in methanol and drying over night in a desiccator. Development with a 18-step gradient from methanol (saturated with ammonia) to tert. butylmethyl ether over 50 mm. Detection by exposure to HCl vapor followed by dipping into a solution of 0.2 g Bratton - Marshall reagent (N-(1-naphtyl)ethylenediaminedihydrochloride) in 100 mL methanol - dichloromethane 1:4. Visual evaluation. Quantitative determination by absorbance measurement at 490 nm. LOD is 1 ng/spot. Linearity is given in the range of 1 - 10 ng amitrol.

J. Pharmaceutical Research 4 (3), 55-57 (2005). HPTLC of indapamide on silica gel with toluene - methanol 7:3. Quantitative determination by absorbance measurement at 246 nm. The method was linear within the range of 1.4-3.7 µg/mL. Mean recovery values were 99.46-100.29 %. The method was validated for accuracy, precision, and specificity.

Benth, identification by TLC fingerprint. Indian Drugs 42 (6), 392-395 (2005). Dried leaves of Pithecellobium Dulce. Benth were successively extracted with n-hexane, chloroform and alcohol. Each extract was evaluated for antimycobacterial activity. These extracts were subjected to TLC fingerprint profile for identification on silica gel with chloroform - methanol 9:1.

Acta Chrom. 11, 51-61 (2001). A homologous series of C4 - C12 aliphatic alcohols was derivatized with dansyl chloride to introduce a fluorophoric group into the molecule which enables TLC and/or HPLC analysis with UV detection. The progress of the reaction under different sets of conditions was monitored by RP-TLC and it was confirmed that the best yield was obtained after 24 h at ambient temperature. TLC on RP-18 with acetonitrile – water 3:2 in a horizontal chamber. Densitometry at 361 nm.

Chem. Pharm. Bull. 53, 691-693 (2005). HPTLC of trans-resveratrol in the roots of Polygonum cuspidatum and in dosage forms on silica gel with chloroform – ethyl acetate – formic acid 25:10:1. Quantitative determination by absorbance measurement at 313 nm. Linearity of determination of trans-resveratrol is between 0.5 and 3.0 µg with a correlation coefficient of 0.9989. LOD is 9 ng, and LOQ is 27 ng. The average percentage recovery is 99.9 - 100.7 %.

extract by planar chromatography techniques (TLC and AMD). J. Sep. Sci. 29, 2245-2250 (2006). HPTLC of four boswellic acids: 11-keto-beta-boswellic acid (1), acetyl-11-keto-beta boswellic acid (2), beta-boswellic acid (3), and acetyl-beta-boswellic acid (4) on silica gel with automated multiple development (AMD) using solvent gradients. Quantitative determination of 1 and 2 by absorbance measurement at 254 nm. 3 and 4 are quantified after derivatization with anisaldehyde sulfuric acid reagent at 560 nm. The AMD system provides good separation and the method is simple, precise, specific, sensitive, and accurate.