Chromatographia 65 (1-2), 105-110 (2007). HPTLC of seven pharmaceuticals, enrofloxacine, oxytetracycline, trimethoprim, sulfamethazine, sulfadiazine, sulfaguanidine and penicillin G/procaine in production wastewater (obtained by solid-phase extraction on hydrophilic-lipophilic balance cartridges with methanol) on cyano phase with 0.05 M oxalic acid - methanol 81:19 after optimization of chromatographic separation by systematic variation of the mobile phase composition using genetic algorithm approach. Quantification by videodensitometry at 254 and 366 nm. Validation of the method by investigation of linearity ranges (1.5 - 15.0 µg/L for enrofloxacine, 100 - 500 µg/L for oxytetracycline, 150 - 600 µg/L for trimethoprim, 300 - 1100 µg/L for sulfaguanidine and 100 - 400 µg/L for sulfamethazine, sulfadiazine and penicillin G/procaine, R > 0.991), its mean recoveries (74.6 - 117.1% and 55.1 - 108.0% for wellspring water and production wastewater, respectively). Application of the method in determination of pharmaceuticals in wastewater samples from pharmaceutical industry.

J. Liq. Chromatogr. & Relat. Technol. 30, 1697-1704 (2007). TLC of capsaicinoids from chili peppers with e. g. capsaicin, dihydrocapsaicin, coumaric acid, vanillin, ferulic acid, and cinnamic acid as standards, on silica gel by two fold development with cyclohexane - chloroform - acetic acid 7:2:1; chloroform - methanol - acetic acid 95:1:5; and cyclohexane - acetone 4:5. Visualization under UV light at 254 nm. Quantitation by densitometry at 254 nm.

J. Chromatogr. A 1155 (1), 112-123 (2007). Presentation of a novel strategy for the fractionation of complex peptide mixtures using two-dimensional planar electrochromatography/thin-layer chromatography (2D PEC/TLC). It was found that phosphopeptides migrate more slowly in the first dimension, based upon their anionic phosphate residues, and certain predominantly acidic phosphopeptides even migrate in the opposite direction, relative to the bulk of the peptides. Further distinguishing phosphopeptides based upon hydrophilicity in the second dimension, which permits a restricted region of the plate to be directly interrogated for the presence of phosphopeptides by MS. Discussion of peptide sequencing and identification of phosphopeptide from the plates by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF)-MS and tandem MS.

J. Planar Chromatogr. 20, 327-330 (2007). TLC of beta-sitosterol on silica gel with toluene - ethyl acetate - glacial acetic acid 15:4:1 with chamber saturation for 30 min. Visualization by spraying with anisaldehyde reagent and heating at 90 °C for 5 min. Densitometric quantitation at 525 nm.

59th Indian Pharmaceutical Congress C-100, 248, (2007). An HPTLC method has been developed for the estimation of flavonoids tannin and saponin in Caesalpinia bonduc. The dried powdered leaves of the plant were extracted with methanol and used for evaluation. HPTLC on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 20:2:2:5 for flavonoids, chloroform - glacial acetic acid - methanol - water 16:8:3:2 for saponin, and ethyl acetate - toluene - formic acid 6:6:1 for tannin. Detection by spraying with 5 % methanolic sulphuric acid for saponin, 5 % alcoholic aluminium chloride solution for flavonoids, and 5 % ferric chloride solution for tannin. Densitometric evaluation at 254 nm and 366 nm.

J. Planar Chromatogr. 20, 179-182 (2007). HPTLC of apigenin on silica gel with chloroform - acetone - formic acid 76:16:8 in a twin-trough chamber saturated for 30 min. Quantification by densitometric scanning at 340 nm.

59th Indian Pharmaceutical congress C- 260, 287, (2007). HPTLC of artemisinin, quercitin and rutin from Artemisia dracunculus (Asteraceae) on silica gel with ethyl acetate - dichloromethane - formic acid - glacial acetic acid - water 100:25:10:10:1 for artemisinin, and n-hexane - acetone - ethyl acetate 16:1:1 for quercitin and rutin. Evaluation under UV 254 nm. The hRf values were 15, 26 and 91 for quercitin, artemisinin, and rutin, respectively. The alcoholic extract (dried) of the plant was found to contain 0.84 % artemisinin, 0.14 % quercitin, and 0.019 % rutin.

Indian J. Pharm. Sci. 69(3), 446 (2007). HPTLC of swetimarin (a phytoconstituent of Enieostemma Littorale) in chloroform extracts of a polyherbal antidiabetic commercial formulation on silica gel with benzene - methanol 4:1. Detection by spraying with 10 % methanolic sulphuric acid and heating at 130° C for 2 min. Evaluation by densitometry at 536 nm. The linearity range was 50-1500 ng/zone. Recovery was more than 96 %.