J. Liq. Chromatogr. Relat. Technol. 29, 1891-1903 (2006). HPTLC of androsterone, epi-androsterone, dehydro-epi-androsterone, testosterone, stigmasterol, beta-sitosterol, estradiol, hydrocortisone, and cholesterol on RP-18 W with methanol - water, and acetonitrile - water in different composition, with chamber saturation. Detection by spraying with sulfuric acid - methanol 1:9 and heating at 120 °C for 15 min. Densitometric determination of RF values. The aim of the work was to compare the lipophilicity of selected steroids determined by RP-HPTLC on RP-18 W plates using different mobile phases with lipophilicity values estimated by computational methods.

J. Liq. Chromatogr. Relat. Technol. 29, 1503-1514 (2006). TLC of tanshinones (cryptotanshinone, tanshinone I, tanshinone II A) on silica gel with light petroleum (60-90°C) - ethyl acetate 4:1. Evaluation and scanning densitometry at 280 nm. With TLC 8 stable components were separated in common within 48 min, respectively, from 3 crude samples of Salvia miltiorrhiza Bunge from different growth locations. With High Speed Countercurrent Chromatography (HSCCC) 12 components were separated, respectively, with good correspondence and precision within 13 h. Both TLCS and HSCCC were effective in showing the whole concentration distribution of all kinds of constituents in different samples. HSCCC showed better performance in analysis of tanshinones, which produced a fingerprint which contained more chemical information than that of TLCS.

J. Sep. Sci. 30, 708-712 (2007). HPTLC of icariin in the aerial part of Epimedium koreanum Nakai on silica gel with ethyl acetate – glacial acetic acid – formic acid – water 10:1:1:2. Quantitative determination by absorbance measurement at 270 nm. The LOD and LOQ for icariin were 66 and 215 ng/band, respectively. Results did not show statistical significance between HPLC and HPTLC.

J. Planar Chromatogr. 20, 127-130 (2007). TLC of isorhamnetin-3-glucoside, isorhamnetin-3-rutinoside, kaempferol-3-rhamnosidoglucoside, isoquercitrin, rutoside, hyperoside on silica gel in horizontal chamber with ethyl acetate - methyl ethyl ketone - formic acid - water 5:3:1:1 or ethyl acetate - formic acid - water 9:1:1. Quantitation by scanning at 366 nm; detection by spraying with a 1 % methanolic solution of Natural Product Reagent A followed by a 4 % methanolic solution of polyethylene glycol 400.

Food Res. Int. 40, 167-175 (2007). HPTLC of absinthin in absinthe beverage (from the wormwood plant Artemisia absinthium L.) on silica gel with acetone - acetic acid (98 %) – toluene – dichloromethane 1:1:3:5. Detection by dipping into a solution of acetic anhydride – sulphuric acid – ethanol 1:1:10, followed by heating for 5 min at 104 °C. Quantitative determination by absorbance measurement at 554 nm. The hRf value of absinthin was 64 and selectivity regarding matrix was given. Linearity was between 0.1 and 10 g/L. The precision was better than 13.5 % (intraday) and 15.8 % (interday). The limit of detection and quantification for absinthin was 0.05 and 0.11 g/L, respectively.

59th Indian Pharmaceutical congress C-323, 302,(2007). TLC of flavonoids and sapogenins from different plant parts (leaves and petals) of Calendula officinialis on silica gel with ethyl acetate - formic acid - glacial acetic acid - water 100:11:11:20, and chloroform - glacial acetic acid - methanol - water 15:8:3:2.

59th Indian Pharmaceutical congress F-9, 392, (2007). HPTLC of sumatriptan succinate in bulk and tablet formulations on silica gel with methanol - water - glacial acetic acid 40:80:1. Densitometric evaluation at 230 nm.

J. Planar Chromatogr. 20, 335-339 (2007). TLC of etoricoxib (rofecoxib as internal standard) on silica gel in a filter-paper-lined twin-trough chamber previously saturated with mobile phase vapor for 30 min with toluene - 1,4-dioxane - methanol 17:2:1. Densitometric analysis of etoricoxib was performed in absorbance mode at 235 nm. The limits of detection and quantitation were 30 and 100 ng/zone, respectively.