Indian J. Pharm. Sci. 66 (3), 278-282 (2004). HPTLC on silica gel with chloroform - acetonitrile - toluene - acetate buffer (pH 6.0) 50:40:10:3. Quantitative determination by absorbance measurement at 266 nm. The method was validated in terms of linearity, accuracy, precision, and specificity. The limit of detection and the limit of quantification were found to be 50 ng/spot and 100 ng/spot respectively. A simple, precise, accurate and rapid HPTLC method has been developed and validated for the simultaneous determination of cefuroxime axetil and probenecid in combined dosage form.

J. Planar Chromatogr. 17, 207-212 (2004). HPTLC of malvidin 3-glucoside, cyanidin 3-glucoside, delphinidin 3-glucoside, peonidin 3-glucoside,and petunidin 3-glucoside on silica gel with ethyl acetate - formic acid - twice distilled water 17:2:3 in an unsaturated twin trough chamber. After drying detection with methanolic 2,2-diphenyl-1-picrylhydrazyl reagent. Quantitative determination by videodensitometry.

IPC 56th 2004, Abstract No. D-9. HPTLC for the standardization of the alkaloid conessine in several market formulations containing kurchi bark, on silica gel with toluene - methanol - chloroform 1:2:1. Detection by spraying with Dragendorff’s reagent. Densitometric evaluation at 460 nm. The method was validated for accuracy, precision, linearity range, specificity, LOD, LOQ and found suitable for routine analysis of herbal formulations containing Kurchi as main ingredient.

IPC 56th 2004, Abstract No. G-5. HPTLC of conessine in Holarrhena antidysentrica, an important ayurvedic drug, on silica gel with toluene - ethyl acetate - diethyl amine 13:5:2. Detection by spraying with Dragendroff’s reagent. Quantitative determination by densitometric scanning at 520 nm. Different market samples of the drug were found to contain 0.30 - 1.46 % of conessine with recovery of 95.18 - 102.70 %

IPC 56th 2004, Abstract No. GP-15. Stability indicating HPTLC determination of ondansetron in solid oral dosage forms on silica gel with chloroform - methanol 4:1. Quantitative determination by scanning at 310 nm. The Rf value was 0.62 - 0.64, linearity was 40 - 120 ng. The average recovery was 100.01 %. The method was found suitable for routine analysis of formulations containing ondansetron.

J. Planar Chromatogr. 18, 437-442 (2005). HPTLC of quercetin and kaempferol in horizontal chambers on silica gel (prewashed with methanol) with four mobile phases, e.g.1,4-dioxane - toluene - 85 % acetic acid 6:24:1 or on cellulose with five mobile phases. Evaluation under UV light at 254 and 366 nm before and after spraying with a 2 % solution of zirconium (IV) dichloride oxide in methanol. Quantitative determination by absorbance measurement at 373 for quercetin and at 347 nm for kaempferol.

CBS 95, 9 (2005). HPTLC of glyphosate and AMPA derivatized in situ on the application position of the plate with FMOC, on silica gel with n-butanol - water - acetic acid 5:1:1 over 70 mm in an unsaturated twin trough chamber. After drying dipping in paraffin - toluene 1:1 for fluorescence enhancement. Quantitative determination by fluorescence measurement with mercury lamp at 265/M 360 nm. Linear calibration using peak height, LOD 0.5 ng absolute per substance zone for glyphosate-FMOC and 0.2 ng for AMPA-FMOC.

J. Planar Chromatogr. 18, 155-159 (2005). TLC of repaglinide on RP-8 with acetonitrile - phosphate buffer pH 6.0 3:2. Quantitative determination by absorbance measurement at 225 nm. Calibration in the range of 0.6-3.6 µg was linear with a good correlation coefficient (r = 0.998 +/- 0.001). Limits of quantitation and detection were 0.27 µg and 0.08 µg, respectively.