60th Indian Pharmaceutical Congress PA-213 (2008). HPTLC of atorvastatin calcium and amlodipine besilate on silica gel with chloroform - toluene - methanol - water 55:10:20:2. Quantitative determination by absorbance measurement at 242 nm. The calibration curve was found to be linear between 400 and 1200 ng/spot for both atorvastatin calcium and amlodipine besilate. The limit of detection and the limit of quantification for atorvastatin calcium were 100 and 400 ng/spot, and for amlodipine besilate 200 and 400 ng/spot, respectively.

Abstract No. 9324, IHCB (2009). HPTLC of hesperidin in orange peel extract and formulation on silica gel with ethyl acetate - methanol - water 100:17:13. Quantitative determination by absorbance measurement at 287 nm. The method was linear in the range of 10-1000 ng/spot. Hesperidin was subjected to degradation studies (acid, alkali, hydrolysis, oxidation, and thermal stress) and was found susceptible to different stress condition. The method was suitable for determination of hesperidin and its degradation products in bulk drug as well as formulations.

60th Indian Pharmaceutical Congress PA-209 (2008). HPTLC of atorvastatin calcium, ramipril and aspirin on silica gel with methanol - benzene - ethyl acetate - glacial acetic acid 9:140:100:1. Quantitative determination by absorbance measurement at 210 nm. The hRf value of ramipril was 6, of atorvastatin 38, and of aspirin 86. Linearity was 100-600 ng/band (atorvastatin), 50-300 ng/band (ramipril) and 500-3000 ng/band (aspirin). Recovery was 99.9-100.0 % for all three compounds. Salicylic acid, an impurity of aspirin, was observed in capsule dosage form with an hRf value of 72. The method was suitable for analysis of combined dosage form.

Indian Drugs 46(6), 477-482 (2009). HPTLC of extracts of Cassia auriculata, C. obtusifolia, and C. uniflora and of chrysophanol and emodin on silica gel with toluene - ethyl acetate - formic acid 10:3:1. Detection under UV 254 nm. Based on the fingerprint and by comparison with chemical markers identification of each species was possible.

Chinese J. Pharm. Anal. 28 (4), 608-610 (2008). TLC of hyperoside in the raw extract of Hypericum perforatum on silica gel with petroleum ether (60-90 °C) – ethyl acetate – methanol 1:4:2. Detection under daylight. Quantification by densitometry at 591 nm. Linearity was given between 0.1 and 14.4 µg/spot (r2 = 0.9892), recovery was between 96.4 and 100.1 %.

J. Braz. Chem. Soc. 21, 441-446 (2010). HPTLC of ochratoxin A in wine on silica gel with toluene - ethyl acetate - chloroform - formic acid 6:3:1. Quantitative determination by absorbance measurement at 366 nm, using a CCD camera followed by images processing using the software ImageJ. Linearity was between 0.8 and 32 µg/L. The intra-day and inter-day precisions had a RSD lower than 9.9 % and 11.5 %, respectively. LOD was 16 ng/zone while LOQ was 100 ng/zone. The proposed method is a simple, efficient and low cost tool for quantitative analysis of ochratoxin A in wine samples.

Abstract No. C-495, 61st IPC (2009). Screening of different phytoconstituents in a polyherbal tablet formulation. TLC of n-hexane, chloroform and methanol extracts of the tablets on silica gel with n-hexane - ethyl acetate 7:3; chloroform-methanol 9:1, and chloroform - glacial acetic acid - methanol - water 8:40:15:10. Evaluation under UV 254 nm as well as under UV 366 nm after spraying with different reagents: 20 % sulfuric acid, aniline-hydrogen phthalate reagent, anisaldehyde-sulfuric acid reagent, and vanillin-sulfuric acid reagent for the detection of piperine and andrographide, the active constituents present in formulations like Tefroliv Forte tablets. Other constituents (tannins etc) were analyzed by UV spectrophotometry.

Abstract No. C-258, 61st IPC (2009). HPTLC of piperine, plumbagine and zingiberine in Panchkol Churna, an ayurvedic preparation used for anorexia, distension and abdominal pain. HPTLC on silica gel with toluene - ethyl acetate 7:3. Densitometric evaluation at 340 nm for piperine, and at 420 nm for plumbagine and zingiberine. The hRf value of piperine, zingiberine and plumbagine was 31, 75, and 84.