CBS 96, 14-15 (2006). TLC of glibenclamide as adulterant in antidiabetic herbal drugs, on silica gel with toluene - ethyl formate - formic acid 5:4:1 in a saturated twin trough chamber over 150 mm. Detection under UV 365 nm, quantification of the image with VideoScan software. Rf of glibenclamide is 0.58. Results were compared with those obtained by UV spectrophotometry and HPLC and showed good correlation.

J. Planar Chromatogr. 19, 238-240 (2006). HPTLC of plant extracts, using pellitorine and dihydropiperlongumine as markers, on silica gel in a twin-trough chamber saturated with the mobile phase hexane - ethyl acetate 3:1. Quantitation by densitometry in absorbance/reflectance mode at 260 nm.

Acta Chrom. 6, 7-12 (1996). HPTLC of fructose, glucose and sucrose on silica gel with concentration zone. Impregnation of the layer (except concentration zone) by spraying with 0.1 M sodium bisulfate solution, followed by drying and spraying with citrate buffer of pH 4.8. Three-fold development with acetonitrile - deionized water 17:3 after chamber saturation. Visualization by spraying with 1-naphthol - sulfuric acid reagent and heating at 110 °C. Quantification by densitometry at 515 nm.

Acta Chrom. 11, 96-101 (2001). HPTLC of the muscle relaxant carisoprodol on silica gel uniplates with inorganic binder and fluorescent indicator, prewashed with dichloromethane – methanol 1:1, with chloroform – acetone 4:1 as mobile phase. Detection by spraying with conc. sulfuric acid – methanol 1:1 followed by heating at 150 ºC for 5 min. Quantitative determination by absorbance measurement at 550 nm. The method was applied to tablets containing carisoprodol as the only active ingredient and to tablets containing carisoprodol with aspirin and with aspirin plus codeine phosphate.

J. Planar Chromatogr. 19, 427-431 (2006). HPTLC of sildenafil citrate on silica gel, pre-washed with methanol, with toluene - acetone - methanol 3:1:1 in a saturated twin-trough chamber. Quantitative determination by absorbance measurement at 312 nm. The method was validated in accordance with ICH guidelines on the validation of analytical methods.

J. Liq. Chromatogr. & Relat. Technol. 29, 2167-2180 (2006). HPTLC of neutral lipids on prewashed silica gel with petroleum ether - diethyl ether - glacial acetic acid 80:20:1; and of methyl and steryl esters with hexane - petroleum ether (35-60°C) - diethyl ether - glacial acetic acid 50:25:5:1 in a twin trough chamber saturated for 20 min. Detection by spraying with a 50 % solution of phosphomolybdic acid in ethanol, followed by heating at 115°C for 10 min. Polar lipids were separated with chloroform - methanol - water 65:25:4. Detection by spraying with 10 % cupric sulfate solution, followed by heating at 140 °C for 10 min. Quantitative determination by absorbance measurement at 610 nm for neutral lipids and at 370 nm for polar lipids.

Ind. J. Pharm. Sci. 68 (6), 838-840 (2006). HPTLC of ofloxacin and ornidazole in tablet dosage form on silica gel with n-butanol - ethanol - ammonia 5:5:4. Quantitative determination by absorbance measurement at 295 nm. The method was found to be linear in the concentrate range of 1-5 ng/spot with recovery of 99.5-102.5 % for both compounds. The method was validated for linearity, accuracy, precision, repeatability, and specificity.

J. Sep. Sci. 30, 1250-1254 (2007). HPTLC of Emblica officinalis extract on silica gel with ethyl acetate – formic acid – glacial acetic acid – water 10:1:1:2. Primary detection under UV 280 nm. Antiradical activity of individual components was estimated on intensity of disappearance of violet/purple background of plate after dipping in DPPH radical solution (0.5 mM in methanol) at room temperature for 90 s and that 30 s at 60 °C. Quantitative determination by absorbance measurement at 517 nm as negative peak. DPPH radical scavenging activity of emblicanins A and B was 7.9 and 11.2 times more active than that of ascorbic acid and 1.3 and 1.8 times more active than gallic acid, respectively.