J. Planar Chromatogr. 18, 437-442 (2005). HPTLC of quercetin and kaempferol in horizontal chambers on silica gel (prewashed with methanol) with four mobile phases, e.g.1,4-dioxane - toluene - 85 % acetic acid 6:24:1 or on cellulose with five mobile phases. Evaluation under UV light at 254 and 366 nm before and after spraying with a 2 % solution of zirconium (IV) dichloride oxide in methanol. Quantitative determination by absorbance measurement at 373 for quercetin and at 347 nm for kaempferol.

CBS 95, 9 (2005). HPTLC of glyphosate and AMPA derivatized in situ on the application position of the plate with FMOC, on silica gel with n-butanol - water - acetic acid 5:1:1 over 70 mm in an unsaturated twin trough chamber. After drying dipping in paraffin - toluene 1:1 for fluorescence enhancement. Quantitative determination by fluorescence measurement with mercury lamp at 265/M 360 nm. Linear calibration using peak height, LOD 0.5 ng absolute per substance zone for glyphosate-FMOC and 0.2 ng for AMPA-FMOC.

J. Planar Chromatogr. 18, 155-159 (2005). TLC of repaglinide on RP-8 with acetonitrile - phosphate buffer pH 6.0 3:2. Quantitative determination by absorbance measurement at 225 nm. Calibration in the range of 0.6-3.6 µg was linear with a good correlation coefficient (r = 0.998 +/- 0.001). Limits of quantitation and detection were 0.27 µg and 0.08 µg, respectively.

and its compound preparations by thin-layer chromatography) (Chinese). Chinese J. Chromatogr. (Sepu) 23 (2), 216-217 (2005). TLC on silica gel by 2-fold development with cyclohexane - ethyl acetate 10:1 followed by cyclohexane - ethyl acetate 2:1. Detection by spraying with H2SO4 - ethanol 1:10 followed by heating at 105 ºC for 5 min. Visualization under UV 365 nm.

Abstract G-25, IPC (2005). HPTLC of cefuroxime axetil in bulk and tablets on silica gel with chloroform - methanol - toluene 2:1:1 with chamber saturation for 30 min. Quantitative determination by absorbance measurement at 290 nm. The linearity was within the range of 300-900 ng/spot with an average recovery rate of 99.4 %. The method was validated as per ICH guidelines.

Chinese J. Chromatogr. (Sepu) 23 (2), 217-218 (2005). TLC of the title compound on silica gel with chloroform - benzene 7:3. Detection 1) by spraying with 10 % H2SO4 in ethanol followed by heating at 120 ºC for 10 min; 2) by spraying with 10 % phosphomolybdic acid in ethanol followed by heating at 120 ºC for 10 min, then exposing to ammonia vapours. Quantification by comparison of the separated zone size with the standard. Optimization of the mobile phase by investigation of the influence of the composition of the developing solvent on Rf values. Optimization of the visualization condition by investigating the relationship between the sample dosage and the visualization results.

J. Planar Chromatogr. 19, 161-166 (2006). HPTLC of 16 amino acids on cellulose in a horizontal chamber with aqueous mixtures of 2-propanol, acetonitrile, and tetrahydrofuran in the range of 60-90 % (10 % increments). Acetic acid (at a concentration of 1%) was only added to the mobile phases containing 2-propanol. Best separation was achieved with tetrahydrofurane - water 17:3; acetonitrile - water 4:1; and 2-propanol - water - acetic acid 89.5 - 9.5 - 1 respectively. The adsorbed solvent was removed before repeating the development process. Visualization by spraying with ninhydrin solution and heating at 105 °C for 1.5-2 min. Densitometric evaluation by absorbance measurement at 415 nm.

Indian Drugs 42 (6), 340-344 (2005). HPTLC of captopril and hydrochlorothiazide in tablets on silica gel with methanol - toluene - ethyl acetate - glacial acetic acid 2:12:6:1. Quantitative determination by absorbance measurement at 219 nm. The Rf value of hydrochlorothiazide was 0.38 and of captopril 0.57. The calibration curve response was 4-14 µg for both drugs. Recovery was determined by standard addition method. The percent recovery by area was found to be 100.25 for captopril and 99.98 for hydrochlorothiazide. The method was suitable for routine quality control of such formulations.