International J. Pharm. & Tech 2(2), 285-292 (2010). TLC of gallic acid in acetone extracts of bark powder of Acacia nilotica on silica gel with toluene – ethyl acetate – formic acid 15:10:2. The hRf value of gallic acid was 36. Quantitative determination by absorbance measurement at 280 nm. The method was linear in the range of 100-350 ng/band with recovery of 97.5 %.

62nd Indian Pharmaceutical Congress Abstract No. F-253 (2010). TLC of ambroxol hydrochloride and doxophylline on silica gel with n-butanol – toluene – ethyl acetate – 25 % ammonia 50:30:20:1. The hRf values were 36 and 45 for doxophylline and ambroxol, respectively. Quantitative determination by absorbance measurement at 258 nm. The method was linear in the range of 300-1100 ng/band for ambroxol and 100-1100 ng/band for doxophylline.

62nd Indian Pharmaceutical Congress Abstract No. F-242 (2010). TLC of gatifloxacin and ornidazole on silica gel with n-butanol – ethanol – 8M ammonia 10:1:3. Quantitative determination by absorbance measurement at 299 nm. The hRf value of gatifloxacin was 27 and of ornidazole 83. The method was found to be linear between 20-100 ng/zone for gatifloxacin and 50-250 ng/zone for ornidazole (r2> 0.99). The limit of detection and quantitation were found to be 4.1 and 12.5 ng/zone, respectively for gatifloxacin and 10.3 and 31.2 ng/zone, respectively for ornidazole.

62nd Indian Pharmaceutical Congress Abstract No. F-251 (2010). TLC of levocetirizine and phenylpropanolamine on silica gel with methanol – ethyl acetate – toluene – ammonia 15:4:5:2. Quantitative determination by absorbance measurement at 210 nm. The hRf value was 30 and 60 for levocetrizine and phenylpropanolamine, respectively. The linearity was in the range of 45-270 ng/band for both drugs.

Abstracts, 42nd Middle Atlantic Regional Meeting of the American Chemical Society, College Park MD, USA, May 21-24 (2011). The four TLC methods for acetaminophen, acetylsalicylic acid, ibuprofen, and chlorpheniramine maleate contained in the Compendium of methods developed by A.S. Kenyon and T.P. Layloff at the US FDA for use in countries with limited resources were transferred to quantitative HPTLC. The used sample preparation methods provide suitable calibration curves covering the range of 70-130 % of the label value of the products. Quantitative determination by absorbance measurement at 254 nm.

J. Planar Chromatogr. 24, 274-280 (2011). Presentation of new reagents and visualization methods which enable the detection of particular substances. The best of them provide high selectivity and low detection limits and lead to a reliable analysis of the developed chromatogram. Three groups of chemical reactions are used for detection: oxidation, reduction, and complexation, as well as precipitation of colored precipitates. Oxidation and reduction are most widely used due to their selectivity, which allows detection and identification of the separated substances. Like this the target substance can be selectively visualized, as for example antioxidants in complex natural matrices. Innumerable chemical, physical, and biological methods of visualization are known. Required are new, more efficient reagents fo a selective visualization of a defined part of a substance or, in some cases, for a specific determination of a specific substance. The article describes the detection of food ingredients, medicines and pharmaceuticals, organic compounds not present in food, and inorganic compounds.

International Journal of ChemTech Research 1(3), 677-689 (2009). HPTLC of rosuvastatin in raw material and tablet dosage formulation on silica gel with ethyl acetate - toluene - acetonitrile - formic acid 60:35:5:2. The hRf value was 85. Quantitative evaluation by absorbance measurement at 243 nm. The method was found to be linear in the range of 318-3816 ng/band. The mean recovery was 99.7 %. The sample was subjected to different stress conditions and the degradation products were well separated from the main drug.

J. Ethnopharmacol. 137, 1337-1344 (2011). HPTLC of phyllanthin (A) and gallic acid (B) in the whole plant of Phyllanthus simplex on silica gel with hexane - ethyl acetate 5:1 for (A) and ethyl acetate - formic acid 44:3 for (B). Detection by spraying with anisaldehyde - sulfuric acid reagent. Quantitative determination by absorbance measurement at 260 and 520 nm. The hRf values of (A) and (B) were 19 and 82, respectively. The amount found in samples was 14.5 % for (A) and 0.7 % for (B).