Ham. Ex D. Don, Myricaceae. J. Planar Chromatogr. 23, 326-331 (2010). HPTLC of myrecitin and stem bark extracts on silica gel with toluene - ethyl acetate - formic acid - methanol 15:15:3:2. Quantitative determination by absorbance measurement at 268 nm. Linearity was between 0.4-2.0 µg/band. The limits of detection and quantitation were 93 ng and 284 ng/zone. The intra-day and inter-day precision of the method was in the range of 0.14-0.55 %. The recovery of myrecitin at three concentrations was in the range of 98.9-100.1 % and the average recovery was 99.3 %.

J. Planar Chromatogr. 24, 406-411 (2011). HPTLC of plant extracts and vasicine on silica gel with ethyl acetate - methanol - ammonia (17.3 % ammonia) 40:10:1 in a twin-trough chamber saturated for 15 min. Quantitative determination by densitometry at 298 nm. The hRf value of vasicine was 47. The limit of detection and the limit of quantification was 40 and 100 ng/zone, respectively. The average intra-day and inter-day precision was 0.7 and 2.2 %, respectively. Linearity was between 100-1300 ng/zone with a correlation coefficient of 0.999 and a %RSD of 2.3 %. Average recovery was 98.9 %.

International Journal of Pharmacy and Pharmaceutical Sciences 3(2), 85-89 (2011). TLC of andrographolide on silica gel with toluene - ethyl acetate - formic acid 10:9:1. Densitometric evaluation at 235 nm before derivatization. Evaluation of the fingerprint profile at 254 nm and after derivatization at 366 nm by spraying with anisaldehyde-sulfuric acid reagent followed by heating at 110 °C.

Research Journal of Pharmaceutical, Biological and Chemical science 2(12), 389-396 (2011). TLC of a polyherbal formulation containing Mucuna pruriens with L-dopa as biological marker on silica gel with n-butanol - water - glacial acetic acid 4:1:1 with chamber saturation for 30 min. Detection by dipping in a 0.5 % solution of ninhydrin in ethanol, followed by heating at 120 °C for 2 min. Quantitative determination of L-dopa by densitometry in absorbance mode at 520 nm. The hRf value of L-dopa was 37. Linearity was given in the range of 600-1400 ng/zone.

J. Planar Chromatogr. 24, 281-289 (2011). TLC of tartrazine (E 102), quinoline yellow (E 104), azorubin (E 122), ponceau 4R (E 124), allura red AC (E129), patent blue V (E 131), and brilliant blue FCF (E133) on RP-18 or cyano phase with methanol - acetate or citric buffer containing diethylamine or octane-1-sulfonic acid sodium salt. Quantitative determination by densitometry in the range of 200-800 nm with a diode array scanner. The LOD and LOQ were 33, 54, 93, 119, 87, 31, 59 and 99, 164, 281, 362, 263, 95, 179 ng/zone for E 102, E 104, E 122, E 124, E 129, E 131, and E133, respectively. The correlation coefficients were 0.9970, 0.9963, 0.9895, 0.9965, 0.9930, 0.9975, and 0.9920, respectively. The linearity was given in the range of 2.5-40 and 2.5-70 µg/mL.

Research Journal of Pharmaceutical, Biological and Chemical Sciences 2(1), 108-110 (2011). TLC of flurbiprofen in raw material and tablet dosage form on silica gel with chloroform - methanol - 25 % ammonia 45:5:3. Quantitative evaluation by absorbance measurement at 247 nm. The method was linear in the range of 50-600 ng/band. The sample was subjected to different stress conditions and all the degradation products were well resolved from the main compound. The method can therefore be used for stability studies.

J. Ethnopharmacol. 137, 99-104 (2011). HPTLC of karanjin in rabbit plasma on silica gel with dichloromethane - toluene - methanol 35:15:3. Qualitative evaluation under UV 366 nm and quantitative determination by densitometry at 366 nm. Linearity was between 1.0 and 15.0 µg/mL. LOD and LOQ were 0.5 and 1.0 µg/mL, respectively. The inter-day and intra-day accuracies were 97.1 % and 92.2 %, respectively. Recovery (by standard addition) was between 98.2 % and 99.9 %.

J. of Chromatogr. A 1241, 103-111 (2012). Development of a simple, fast, and efficient HPTLC method for the simultaneous quantitative determination of alcohols and acetates in Haitian vetiver essential oils (Chryzopogon zizanioides) and its acetylated form. TLC on silica gel with n-hexane – chloroform – ethyl acetate 16:12:1 at 47 % relative humidity. Detection with vanillin – sulfuric acid reagent. Quantification of the analytes by densitometry in absorbance mode at 530 nm. Preparation of the reference mixtures of alcohols and acetates by fractionation of Haitian vetiver oil or vetiver acetates, followed by purification of the fractions of interest by means of OPLC. Determination of the chemical composition of each reference fraction by using GC-MS and GC×GC-MS, and of their overall purity by HPTLC with good linearity (r2 = 0.9995 for alcohols, r2 = 0.9996 for acetates) in a concentration range of 40–200 ng/zone.