CBS 94, 14-15 (2005). HPTLC of sucralose on amino phases with acetonitrile - water 4:1 over 70 mm. Stability of sucralose in Burfi, a milk-based confection, was determined over a defined time period. The 2 products of hydrolysis have been monitored as well. Detection by heating for 20 min at 190° C. Quantitative determination by fluorescence measurement under UV 366. Limit of quantitation in the lower ng range.

J. Planar Chromatogr. 17, 355-359 (2004). HPTLC of aristolochic acid A, B, and C and numerous plant extracts on silica gel in a saturated twin-trough chamber using the upper phase of the mixture toluene - ethyl acetate - water - formic acid 20:10:1:1. Quantitative determination by fluorescence measurement at 366 nm after derivatization with tin(II) chloride reagent. The working range and linearity, LOD and LOQ (based on the calibration plot), and precision (n = 6), were validated with methods described by K. Ferenczi-Fodor et al., J. AOAC Int. 84 (2001) 1265-1276. The stability of the analyte during chromatography was established by two dimensional chromatography. The new method enables visual detection of the acids with certainty at very low levels (400 pg absolute of aristolochic acid A) in plant material and can therefore be used for screening Chinese drugs to ensure their safety on the basis of absence of aristolochic acid.

56th IPC 2004, Abstract No. GP-48. HPTLC of rabeprazole sodium in tablet dosage form on silica gel with ethyl acetate - methanol 9:1. Optimization of experimental parameters such as bandwidth, chamber saturation time, solvent front migration, and mobile phase composition. Quantitative determination by scanning at 260 nm. The Rf value was 0.59. The method was linear with a correlation coefficient of 0.99, recovery was 98.81 %.

Chinese J. Trad. Pat. Med. (Zhongchengyao) 27(1), 34-37 (2005). TLC on silica gel with 1) benzene - acetone - ethyl acetate - ammonia water 10:15:20:1; 2) ethyl acetate - butanone - formic acid - water 5:3:1:1; 3) n-butanol - glacial acetic acid - water 7:1:2. Detection 1) by spraying with potassium iodobismuthate solution; 2) by spraying with 2 % FeCI3 in ethanol; 3) by spraying with vanillin - H2SO4 solution and heating; 4) under UV light. Identification by fingerprint technique. Quantification of geniposide by HPLC. The results for ten real life samples are given.

J. Chromatogr. A 1026 (1-2), 301-304 (2004) A new and simplified method for extraction of ergosterol (ergosta-5,7,22-trien-3beta-ol) from fungi in soil and litter was developed using pre-soaking extraction and paraffin oil for recovery. Recoveries of ergosterol were in the range of 94-100 % depending on the solvent to oil ratio. Extraction efficiencies equal to heat-assisted extraction treatments were obtained with pre-soaking extraction. Ergosterol was detected by TLC. Detection by fluorescence measurement, quantification limit was 8 ng. Using visual evaluation of images of TLC plates photographed in UV-light the quantification limit was 16 ng.

J. Planar Chromatogr. 18, 349-357 (2005). HPTLC of pesticides (prochloraz, chloroxuron, chlorotoluron, metoxuron, chlorbromuron, cyanazine, flamprop-M-isopropyl, prometryn, permethrin, bitertanol, hexazinone, chlorsulfuron, methabenzthiazuron, phenmedipham, metiocarb, dichlofluanid, propachlor, procymidone, terbuthylazine, propyzamide, tri-allate, bifenthrin) on silica gel, RP-18 W, RP-18, and cyano phases in a horizontal chamber. In adsorbent-gradient TLC silica gel plates were used in the first direction with tetrahydrofuran - n-heptane 1:4. After drying, the plates were cut in narrow strips and connected with RP-18 W or cyano plates, the pesticides were transferred to the before mentioned plates with methanol and developed in the second dimension with water - methanol or water - dioxane. Detection under UV light at 254 or 366 nm. Quantitative determination by densitometry at 254 nm.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27(5), 529-532 (2005). TLC of extracts on silica gel developed with 1) ethyl acetate - ethanol 4:1; 2) ethyl acetate - methyl ethyl ketone - formic acid - water 10:1:1:1; 3) cyclohexane - acetone 10:3. Detection 1) under UV 254 nm; 2) by spraying with 5 % AICl3 in ethanol followed by heating at 105 ºC for 5 min, and inspection under UV 365 nm; 3) by spraying with 5 % vanillin in H2SO4 followed by heating at 105 ºC until the spots are visualized. Identification by fingerprint technique. Quantification of ginsenoside Rg1 by HPLC. The results for three real life samples are given.

J. AOAC Int. 88, 70-79 (2005). HPTLC of haloperidol, amitriptyline, sulpiride, promazine, fluphenazine, doxepin, diazepam, trifluoperazine, clonazepam, and chlorpromazine in 25 selected psychotropic drugs on silica gel with 30 mobile phases, eight of them were selected based on spot location and developing time. Identification and quantification were carried out based on UV densitometric measurements. In addition to retention factors, the absorption spectra recorded directly from chromatograms were also used in qualitative analysis. Limit of detection ranged from 0.009 to 0.260 µg depending on the wavelength used. A satisfying recovery, ranging from 92.9 to 104.7 %, was achieved for individual constituents.