J. Planar Chromatogr. 18, 285-289 (2005). HPTLC of glucose - after hydrolyis of starch using alpha-amylase and amyloglucosidase - on silica gel, pre-washed with methanol, treated by immersion in a 0.1 M solution of di-potassium hydrogen phosphate in methanol and activated for 30 min at 120 °C. Three-fold development was performed in a horizontal development chamber with acetonitrile - water 17:3. Detection by dipping in aniline-diphenylamine reagent, densitometry at 520 nm. Calibration was linear between 100 and 300 ng with a coefficient of determination r2 of 0.9959. The limits of detection and quantification for starch as glucose were 0.26 and 0.51 g/100 g, respectively.

J. Planar Chromatogr. 18, 85-88 (2005). 11 HPTLC of fenoxaprop-p-ethyl and degradation products on silica gel (prewashed with methanol - chloroform 1:1 and activated at 110 °C for 30 min) in a twin-trough chamber with toluene - dichloromethane 7:3. Visualization on irradiation with an UV lamp at 236 nm. Quantitative determination by absorbance measurement at 236 nm.

J. Pharm. Biomed. Anal. 37, 817-821 (2005). CO2 extracts of Harpagophytum procumbens root was evaluated by HPLC and HPTLC for harpagoside contents. HPTLC on silica gel with ethyl acetate - methanol - water 77:15:8 in saturated ADC chamber. Detection by dipping into anisaldehyde reagent followed by drying at 120 °C for 5 min. Quantitative determination by absorbance measurement at 509 nm. The linearity range was 0.04-0.40 mg/mL. The HPTLC method was less time consuming than HPLC, needing almost no sample pre-treatment. 15 different CO2-extracts of the plant were analysed.

Abstract DP-41, IPC (2005). HPTLC of solanesol in n-hexane extracts of shade dried and freeze dried leaves of Solanum tuberosum, S. trilobactum, S. xanthocarpum, S. nigrum, and S. toruum on silica gel with chloroform - ethanol 48:1. Quantitative determination by absorbance measurement at 254 nm. Solanum tuberosum contained the highest amount of solanesol out of the 5 analyzed species.

J. Chinese Trad. Patent Med. (Zhongchengyao) 27(5), 535-538 (2005). TLC of the extracts on silica gel with 1) benzene - ethyl acetate - formic acid 15:2:1; 2) n-hexane - ethyl acetate - formic acid 60:20:1; 3) chloroform - methanol - ammonia 40:10:1; 4) chloroform - methanol - water 13:7:2. Detection 1) under UV 365 nm; 2) by spraying with 3 % ninhydrin solution followed by heating at 105 ºC until the spots are visualized; 3) by spraying with 10 % H2SO4 solution in ethanol followed by heating until the spots are visualized. Identification by fingerprint technique. Quantification of emodin by densitometry at 445 nm. Validation of the method by investigation of its linearity range (0.1 µg - 1.0 µg, r = 0.998); precision (RSD = 1.05 % n = 6); reproducibility of six time assay towards the same sample (RSD = 1.24 %); and standard addition recovery (96.7 %, RSD = 1.75 %, n = 6). The results for three batches of real life sample are given.

J. Planar Chromatogr. 18, 57-60 (2005). HPTLC of the organic components of composite modified double-base (CMDB) propellants (with nitroglycerine, carbamite, diethyl phthalate, dibutyl phthalate, 2-nitrodiphenylamine as standards) on silica gel with chloroform - cyclohexane 7:3. Detection under UV light at 254 nm. Densitometric scanning in absorbance mode at 210 nm. Comparison of the UV spectra of the separated compounds with those of the standards.

J. Liq. Chrom. & Rel. Technol. 26, 3453-3462 (2003). HPTLC of caffeine and acetaminophen on silica gel in a saturated twin-trough chamber with ethyl acetate - glacial acetic acid 19:1. Quantification at 254 nm. Diphenhydramine, pseudoephedrine, and acetaminophen were well separated from the caffeine zone. Precision (relative standard deviation) was 1.19 %; limit of detection was 0.2 µg for caffeine and 0.08 µg for acetaminophen; precision of duplicate samples (RSD) ranged from 0.95 to 7.56 %.

Chromatographia 64 (1-2), 101-104 (2006). TLC of metformin and glyburide in three different formulations of Glucovance®, on silica gel with water - methanol - ammonium sulfate 4:2:1. Rf value of metformin was 0.43 and of glyburide 0.64. Quantitative determination by absorbance measurement at 237 nm. The linear regression data for the calibration plot showed a good relationship with r = 0.99581 and 0.99982 for metformin and glyburide, respectively. The method was validated for precision and recovery. The limits of detection and quantification were 25 and 84 ng/spot for metformin and 12 and 41 ng/spot for glyburide, respectively. Stability study has been carried out for samples and standard solutions.