J. Chinese Trad. and Herb. Drugs (Zhongcaoyao), 36 (2), 222-224 (2005). TLC on silica gel with chloroform - methanol - water 70:35:4. Detection by spraying with 10 % H2SO4 in ethanol and heating at 105 °C for 5 min. Identification by fingerprint technique. Quantification by densitometry at 530 nm. Validation of the method by investigation of linearity (0.458 µg - 2.748 µg, r = 0.998); precision (RSD = 2.48 %, n = 5 within plate and RSD = 4.69 % plate to plate); reproducibility of five time assay towards the same sample (RSD = 2.41 %); and standard addition recovery (96.11 %, RSD = 1.91 %, n = 5). The results for real life samples are given.

J. Planar Chromatogr. 17, 224-228 (2004). TLC of fluvoxamine and moclobemide on silica gel in horizontal chambers with benzene - acetone - ethanol - 25 % ammonia 9:7:2:1. Densitometric detection and quantification were performed at 249 nm and 236 nm, respectively. The range of linearity was 1 - 10 µg per spot; the RSD was less than 2.5 % for densitometry and less than 5.1 % for videodensitometry.

J. AOAC Int. 88, 1537-1543 (2005). HPTLC of caffeine, acetaminophen and acetylsalicylic acid on silica gel with concentration zones using methanol - ethyl acetate 3:17. UV absorption densitometry is used for the quantitative determination of caffeine. Precision, accuracy, linearity, limits of detection and quantitation, and selectivity were validated. - A comparative study using a caffeine standard solution and a multicomponent analgesic tablet solution containing caffeine, acetaminophen, and acetylsalicylic acid showed that manual application on the concentration zone, and instrumental application on the silica gel gave quite similar results in terms of number of theoretical plates, resolution, limit of detection, and linearity.

J. Planar Chromatogr. 18, 176-180 (2005). OPLC separation of alkaloids on silica gel using different mobile-phase velocities from 100 to 400 µL/min and investigation of this effect on properties such as retardation factors, reproducibility, efficiency, number of theoretical plates, HETP, and resolution. OPLC of tertiary alkaloid standards (allocryptopine, protopine, chelidonine) and quaternary alkaloid standards (chelerythrine, chelilutine, sanguinarine, and chelirubine) on silica gel with toluene - ethyl acetate - methanol 14:3:3 for tertiary alkaloids and toluene - ethyl acetate - methanol 83:15:2 for quaternary alkaloids.

J. Planar Chromatogr. 18, 269-273 (2005). HPTLC of flavonoids (hyperoside, isoquercitrin, luteolin, luteolin 7-O glucoside, rutin, vitexin, quercetin, quercitrin as standards) and phenolic acids (caffeic and chlorogenic acid) on silica gel after presaturation with ethyl acetate - acetic acid - formic acid - water 100:11:11:26. Detection by spraying with natural products reagent, followed by spraying with PEG. Visualization under UV light at 254 and 366 nm. Quantitative evaluation by video-densitometry.

J. AOAC Int. 88, 1525-1529 (2005). HPTLC of trandolapril and verapamil in 2-component mixtures and in their combination capsules on silica gel in horizontal chambers with ethyl acetate - ethanol - acetic acid 16:4:1. Quantitative determination by densitometric measurement at 215 nm. Detection and quantitation limits were found to be 1.25 and 3.75 µg/spot for TRA and 0.15 and 0.45 µg/spot for VER, respectively.

Abstract CP-31, IPC (2005). HPTLC of stigmasterol in leptadernia reticulata on silica gel with n-hexane - ethyl acetate 4:1. Quantitative determination by absorbance measurement at 525 nm after derivatization. Both hydrolyzed and unhydrolyzed samples (2N HCl) were analysed. Unhydrolyzed samples were found to contain a higher amount of stigmasterol. Linearity was in the range of 0.16-0.48 mg/mL. Several market samples were analysed by the proposed method.

leaf powder by high-performance thin-layer chromatography. J. Planar Chromatogr. 18, 305-307 (2005). HPTLC of methanolic extracts of Cinnnamomum tamala leaves and eugenol on silica gel with toluene - ethyl acetate - formic acid 90:10:0.1. Detection and quantitation by densitometry at 280 nm.