International Journal of ChemTech Research 1(4), 931-936, (2009). TLC of quercetin in hydroalcoholic extracts of dried flower of Nymphaea stellata (Nymphaceae). Separation on silica gel with toluene - ethyl acetate - formic acid 25:20:1. The hRf value was 26. Densitometric evaluation at 380 nm. The method was linear in the range of 20-200 ng/band. The average recovery was 99.3 %.

International Journal of PharmaTech Research 03, 527-538 (2009). A stability indicating HPTLC method has been developed for analysis of cephalexin in bulk and dosage formulation. HPTLC on silica gel with ethyl acetate - methanol - 25 % ammonia 6:4:1. The hRf value was 56. Densitometric quantification at 260 nm. The method was linear in range of 500-1500 ng/band. Cephalexin was subjected to forced degradation (acid, alkali, oxidation, thermal, photolytic). All degradation products were well separated from the drug, indicating specificity of the method.

Biochem. Biophys. Res. Commun. 399, 150-154 (2010). HPTLC of the lipids from mouse primary macrophages on silica gel with butanol - acetic acid - water 3:1:1. Detection by imaging using Fujifilm intelligent dark box in SYBR green fluorescent light. Quantitative determination with a TLC plate imaging software.

J. Planar Chromatogr. 23, 293-297 (2010). TLC of eugenol and piperine on silica gel with toluene - ethyl acetate 9:3 with chamber saturation for 15 min at room temperature. The hRf value of eugenol was 73 and of piperine 28. Linearity was between 1 and 12 µg/band for both eugenol and piperine. A good linear relationship with r2 = 0.9979 and 0.9980 for eugenol and piperine was obtained. The limit of detection was 0.1 ng/band for eugenol and 10 ng/band for piperine. Recovery of eugenol and piperine was above 97 %. The recovery (measured at different concentration levels) of eugenol was 95.9- 97.5 % and of piperine 91.8-96.6 %. For eugenol the robustness of the method (% RSD, n = 3) was 0.74 % (12 ng/band) and 0.69 % (25 ng/band) for toluene - ethyl acetete 9:3 and 0.79 % (12 ng/band) and 0.88 % (25 ng/band) for toluene - ethyl acetate 9:2. For piperine the robustness of the method (% RSD, n = 3) was 0.74 % (5 ng/band) and 0.89 % (10 ng/band) for toluene - ethyl acetate 9:3 and 0.72 % (5 ng/band) and 0.73 % (10 ng/band) for toluene - ethyl acetate 9:2.

Scholars Research Library 2(3), 163-167 (20100. TLC on silica gel with n-butanol - water - methanol - ammonia 8:1:1:2 with chamber saturation for 10 min. The hRf value was 33 for gatifloxacin and 89 ambroxol HCl. The method was linear in the range of 100-600 ng/band for gatifloxacin and 12.5-62.5 ng/band for ambroxol HCI. The recovery was 99.3-100.3 %. Densitometric evaluation at 310 nm.

Eurasian J. Anal. Chem. 4(1), 87-97 (2009). HPTLC of rabeprazole sodium on silica gel with acetone - water 7:30. The hRf value was 45. Densitometric quantification at 284 nm. Linearity was in the range of 400-2400 ng/band. The recovery was 99.6-101.4 %. The sample was subjected to forced degradation (acid, alkali, oxidative, thermal, photolytic). The degradation products were well separated from the main compound and the method is suitable for stability studies.

Chinese J. Chromatogr. 26 (1), 50-55 (2008). TLC of some endophytic fungi isolated by column chromatography from selected medicinal plants on silica gel plates with 1) chloroform - methanol 7:1, 2) chloroform - acetonitrile 7:3, 3) ethyl acetate - 2-propanol 19:1, 4) methylene chloride - tetrahydrofuran 3:1, 5) methylene chloride - methanol - dimethylformamide 90:9:1. Detection by spraying with 1 % vanillin sulfuric acid reagent after gentle heating. Also HPTLC on silica gel with chloroform - methanol 9:1. Detection by densitometry at 254 nm and 366 nm. If taxol was present, derivatization by spraying with 1 % vanillin sulfuric acid reagent and heating for 2 min, then detection under UV 366 nm and white light. Only 13 fungal species produced taxol in the artificial culture medium of the 20 screened fungi.

Indian J. Pharma. Educ. Res. 44(4), 341-344 (2010). HPTLC of silica gel with toluene - ethyl acetate - methanol - formic acid 5:16:21. The hRf value was 51. Densitometric evaluation at 220 nm. The method was linear in the range of 300-1800 ng/band. The sample was subjected to different stress conditions (acid, base, oxidative, thermal, and photolytic). Degradation products were well separated from trandolapril, therefore the method can be used for stability studies.